Nop2 (NM_001191785) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Nol1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217194 representing NM_001191785 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGCAAGTTGGATCCTACGAAGAAGGAGAAGCGTGGGCCCGGCCGAAAGGCTCGAAAGCAGAAGG GTGCAGAGACCGAACTGATCAGATTTTTGCCTGCAGCTGGTGATGAGAATTTCAAGAGGCTGTCCAGTCG TGCTCGGAAGAGGGCGGCCAAGAGGAGGTCAGGGTCTGTTGAAGTCCCTAAGCCAAATAAGTCTCCTGGG ATCAAAACATTGCCTGGAGAGTTACCCAAAGGAGCAGTCCAGGTTCGAGGTAAGAAGCGTCCAGCACCAA TTCAAAACAGTGATGGGGATGAGGAGGAAGACTCTGAGGAAGAAGATGTGGTGACCCAGGGGGAACTTTG GGGCTCTGAAGACAGTAGTGAAGATATGGTGGACGACTATGGAGCGGACTCGGAGGATGAGGAGGAAAAG TTGTTGCCTATTGAAAGAGCTGCTCTGAAGCAGAAGGCCCGGGACCCCGCAGATGGGACCCAGTGGAGTG AGGAGGACACTGATGAAGATGAAGGTGTTACCCCTGAGTCCCACCCCAGAAAGGATGACAAAGCAGAGGG GGATTTGCAGATCAATGTGGAAGAGGAGGAAGCCTTCGTGCTGCCTCCTGCTGGACAGACGGACCAGGAT GCCCAGACTCCAGACCTGCAGCGAGTTCACAAGCGCATCCAGGATATAGTAGGAGTGCTTCGGGATTTTG GGGCTCAGCGAGAGGAAGGCCGGTCTCGAACTGAGTATCTGAGTCAGCTTCAGAAGGATCTGGCCACCTA CTATTCTTATGGAGACTTCCTGTTGAGCAAGCTCATGGAGCTGTTCCCGCTGTCTGAGTTGATAGAGTTC TTAGAAGCTAATGAGGTGCCCCGGCCAATCACCCTTCGGACCAACACCTTGAAAACCCGTCGCCGAGACC TTGCTCAGGCTCTGATCAATCGTGGGGTTAATCTGGATCCATTGGGAAAGTGGTCGAAGTCTGGACTTGT GGTATATGATTCTTCAGTGCCTATTGGTGCTACTCCCGAGTACCTAGCTGGACACTATATGCTGCAGGGA GCTTCGAGCATGCTGCCTGTCATGGCCCTGGCACCTCAGGAGCATGAGCGGATCTTGGACATGTGTTGTG CTCCCGGAGGAAAGACCAGCTACATAGCTCAGCTGATGAAGAACACGGGTGTGATCCTTGCCAATGACGC CAATGCAGAGCGGCTCAAGAGTGTCGTGGGCAACCTGCACCGCTTGGGAGTCACCAACACCATCGTCAGC CACTATGATGGGCGCCAGTTCCCCAAGGTGGTGGGCGGCTTTGATCGGGTGCTTCTGGACGCTCCTTGCA GTGGCACAGGAGTCATCTCCAAAGACCCTGCTGTGAAGACGAACAAGGATGAGAAGGACATCCAGCGCTG TGCCCACCTTCAGAAGGAATTGCTGCTCAGTGCTATAGACTCAGTCAACGCTGCCTCCAAGACCGGAGGC TACCTGGTCTACTGCACTTGCTCCATTACGGTGGAAGAGAACGAGTGGGTGGTGGACTATGCCTTGAAGA AGAGGAATGTGCGGCTGGTGCCCACCGGCCTGGACTTCGGGCAGGAAGGTTTCACCCGCTTCCGAGAAAG GCGCTTTCACCCCACTCTGCGGGCCACCCGACGCTTCTACCCTCACACTCACAATATGGATGGTTTCTTT ATAGCCAAGTTCAAGAAATTTTCCAATTCTATCCCCCAGCCCCACTCAGGAAACTCGGCAGCAGGTACTC CTACAGAACCTGACCTGAAGGGTCAGGTCACCCCAAAGTCTGAGAACAGTAGTCAGCCCACCAAGAAAGC CCACGGGGCTGCAAAGGCAAAGCAGCAGTTAGTAGGGCAGCAACATTCCAAGAAGCCCTTCCAGAAGATG AATGGCATCTCCAAAGGGCCAGGCTTGCCCACTGAACCTTCTGTCCCAAATGTCCAAGTGTCTGCTAGGC CCCAGGAAAGCAGTCTGTCTGATGTGAAACGGAAGAGGGCTGGAAAGCTGAAGCAGCGGGGACCGAAGCA GAGTGCCCCTCCCCCCAAGGTCGGGGACCTGGGGACACCTGCTATGCAGACTCCCTCTGAAATCAGTGCC ACCCCAAGGCCTAAGTCCCCTGGGAAAGCCAAAAAGAGAGAGGAAGTAAAGCAGGAGTTACCAGAGCAGC CTGCCAAGGGAACTGCTTCCCTGAAAGAAGACGCTGCCCCCAAGAGACTCTCTTCCCCCACCACGGCTCA CCACAGTTCCACCAGGCCCCCACCGGCAAAGAGGAGGAAATCTATGACCAAGGGCAGCAGCCAGCCACTG TTGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217194 representing NM_001191785 Red=Cloning site Green=Tags(s) MGRKLDPTKKEKRGPGRKARKQKGAETELIRFLPAAGDENFKRLSSRARKRAAKRRSGSVEVPKPNKSPG IKTLPGELPKGAVQVRGKKRPAPIQNSDGDEEEDSEEEDVVTQGELWGSEDSSEDMVDDYGADSEDEEEK LLPIERAALKQKARDPADGTQWSEEDTDEDEGVTPESHPRKDDKAEGDLQINVEEEEAFVLPPAGQTDQD AQTPDLQRVHKRIQDIVGVLRDFGAQREEGRSRTEYLSQLQKDLATYYSYGDFLLSKLMELFPLSELIEF LEANEVPRPITLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKSGLVVYDSSVPIGATPEYLAGHYMLQG ASSMLPVMALAPQEHERILDMCCAPGGKTSYIAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIVS HYDGRQFPKVVGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDIQRCAHLQKELLLSAIDSVNAASKTGG YLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPTLRATRRFYPHTHNMDGFF IAKFKKFSNSIPQPHSGNSAAGTPTEPDLKGQVTPKSENSSQPTKKAHGAAKAKQQLVGQQHSKKPFQKM NGISKGPGLPTEPSVPNVQVSARPQESSLSDVKRKRAGKLKQRGPKQSAPPPKVGDLGTPAMQTPSEISA TPRPKSPGKAKKREEVKQELPEQPAKGTASLKEDAAPKRLSSPTTAHHSSTRPPPAKRRKSMTKGSSQPL LS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191785
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191785.1, NP_001178714.1
RefSeq Size	2544 bp
RefSeq ORF	2319 bp
Locus ID	314969
MW	85 kDa

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