Cdh10 (NM_001168631) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217209 representing NM_001168631 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAATCTATTCGTTCTTGAGACTGTTTGTTCTCTGGGCATGCCTGCCCCAATTCTGCTGTCCAGAAT TAACGTTCAGAAGGACTCCTGGGCTTCAGCAAATGACTGCAGAATCCCATGTCCCTAGGGGTGATGGTAA AATCCTTCACCGTCAAAAACGTGGCTGGATGTGGAACCAGTTTTTCTTACTGGAAGAATATACTGGATCT GATTATCAATATGTCGGCAAGCTTCATTCAGACCAAGATAAAGGAGACGGATCGCTCAAATATATTTTGT CTGGAGATGGAGCTGGTACTCTTTTTATTATTGATGAAAAAACAGGTGATATTCACGCCACAAGAAGAAT TGATAGGGAAGAAAAGGCCTTTTATACTCTGCGTGCACAAGCTATTAACAGAAGAACTCTGAGGCCAGTT GAGCCAGAGTCAGAATTTGTGATCAAAATTCACGATATCAACGACAATGAGCCTACATTCCCAGAAGAAA TTTATACAGCCAGCGTTCCTGAAATGTCTGTTGTAGGTACTTCTGTGGTGCAAGTCACAGCTACAGATGC CGATGACCCTTCATATGGAAACAGCGCCAGAGTCATTTACAGCATACTTCAAGGGCAGCCTTATTTCTCT GTGGAACCAGAAACAGGTATCATAAGGACAGCTCTACCAAACATGAACAGAGAGAACAAGGAACAGTACC AGGTGGTTATTCAAGCCAAGGACATGGGCGGTCAGATGGGGGGTCTGTCTGGAACCACCACAGTGAACAT CACTCTCACAGATGTCAACGACAATCCTCCTCGCTTCCCCCAAAACACCATCCATCTGCGAGTTCTTGAA TCCTCTCCAGTTGGCACAGCTGTGGGAAGTGTAAAAGCCACCGATGCTGACACGGGGAAAAATGCCGAAG TGGATTACCGCATTATTGATGGAGATGGCACAGATATGTTTGACATTATAACTGAGAAGGACACACAGGA AGGCATCATCACTGTGAAAAAGCCACTTGACTATGAGAACCGAAGACTATATACTCTGAAGGTGGAGGCA GAAAATACCCATGTGGATCCACGTTTTTACTATTTAGGGCCATTCAAAGATACAACAATTGTAAAAATCT CCATAGAAGACGTGGATGAGCCTCCAGTTTTCAGTCGATCCTCCTATCTGTTTGAGGTTCATGAGGATAT TGAAGTGGGCACAATCATCGGTACTGTAATGGCAAGAGACCCAGATTCTACTTCCAGTCCCATCAGATTT ACTTTAGATCGCCATACTGATCTTGACAGGATCTTTAACATTCATTCTGGAAACGGATCACTTTATACAT CAAAGCCACTTGATCGTGAACTATCTCAATGGCACAACCTTACCGTCATAGCTGCCGAGATCAATAATCC TAAAGAAACAACTCGTGTGTCTGTTTTTGTGAGGATTTTGGATGTTAATGACAACGCTCCACAATTTGCT GTGTTTTATGACACATTTGTATGTGAAAATGCCAGACCAGGACAGCTGATACAGACAATAAGTGCAGTTG ACAAAGATGACCCCTTAGGTGGACAGAAGTTCTTCTTCAGTTTGGCTGCTGTGAATCCTAACTTCACAGT GCAAGACAATGAAGACAACACTGCCAGAATTTTAACCAGAAAGAATGGCTTCAACCGTCATGAAATAAGC ACCTACCTACTGCCGGTAGTGATATCTGATAATGACTACCCCATTCAGAGCAGCACTGGCACCCTGACGA TCCGTGTTTGCGCCTGTGACAGCCAGGGCAACATGCAGTCCTGCAGTGCCGAAGCCCTGCTCCTTCCTGC TGGCCTCAGCACTGGCGCCTTGATCGCCATTCTTCTCTGCATCATCATTCTGCTGGTTATAGTAGTCCTC TTTGCAGCCCTGAAAAGGCAACGGAAGAAAGAGCCTCTGATTTTATCCAAAGAAGACATCAGAGACAACA TTGTGAGCTATAACGACGAAGGTGGCGGAGAGGAGGACACCCAAGCCTTTGATATTGGAACCCTGAGGAA TCCTGCAGCTATCGAGGAGAAAAAGCTGCGGCGAGATATCATTCCTGAAACGTTATTTATACCGCGGCGG ACTCCTACGGCCCCGGATAACACGGATGTCCGGGATTTCATTAATGAGCGCCTCAAAGAGCACGACTTGG ACCCCACTGCGCCTCCCTACGACTCGCTGGCTACCTATGCCTATGAAGGAAACGACTCCGTTGCTGAATC TCTGAGCTCCTTAGAATCAGGTACCACTGAAGGAGACCAAAACTACGATTACCTTCGAGAATGGGGGCCT CGGTTTAATAAACTAGCAGAAATGTACGGTGGTGGTGAGAGCGACAAAGACGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217209 representing NM_001168631 Red=Cloning site Green=Tags(s) MSIYSFLRLFVLWACLPQFCCPELTFRRTPGLQQMTAESHVPRGDGKILHRQKRGWMWNQFFLLEEYTGS DYQYVGKLHSDQDKGDGSLKYILSGDGAGTLFIIDEKTGDIHATRRIDREEKAFYTLRAQAINRRTLRPV EPESEFVIKIHDINDNEPTFPEEIYTASVPEMSVVGTSVVQVTATDADDPSYGNSARVIYSILQGQPYFS VEPETGIIRTALPNMNRENKEQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQNTIHLRVLE SSPVGTAVGSVKATDADTGKNAEVDYRIIDGDGTDMFDIITEKDTQEGIITVKKPLDYENRRLYTLKVEA ENTHVDPRFYYLGPFKDTTIVKISIEDVDEPPVFSRSSYLFEVHEDIEVGTIIGTVMARDPDSTSSPIRF TLDRHTDLDRIFNIHSGNGSLYTSKPLDRELSQWHNLTVIAAEINNPKETTRVSVFVRILDVNDNAPQFA VFYDTFVCENARPGQLIQTISAVDKDDPLGGQKFFFSLAAVNPNFTVQDNEDNTARILTRKNGFNRHEIS TYLLPVVISDNDYPIQSSTGTLTIRVCACDSQGNMQSCSAEALLLPAGLSTGALIAILLCIIILLVIVVL FAALKRQRKKEPLILSKEDIRDNIVSYNDEGGGEEDTQAFDIGTLRNPAAIEEKKLRRDIIPETLFIPRR TPTAPDNTDVRDFINERLKEHDLDPTAPPYDSLATYAYEGNDSVAESLSSLESGTTEGDQNYDYLREWGP RFNKLAEMYGGGESDKDA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001168631
ORF Size	2364 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001168631.1, NP_001162102.1
RefSeq Size	3169 bp
RefSeq ORF	2367 bp
Locus ID	29181
MW	88.2 kDa
Gene Summary	cell-cell adhesion molecule [RGD, Feb 2006]

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