Alpk3 (NM_001191895) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1309860
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217679 representing NM_001191895 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCGGAGGGCCTTGGGCCGGGGCTGGGGCCTGGGTGGTCGAGCAGGAACCGGTGGAGATGGCG AGGACGACGGGCCGGTGTGGACTCCTGGCCCAGCCAGTCGCAGCTACTTGCTCAGCGTGCGGCCGGAGGC TAGCTTATCAAGCAACCGGTTGTCTCACCCCAGCTCTGGAAGGAGCACCTTCTGCTCCATCATTGCTCAG CTTACAGAGGAGACCCAGCCGCTGTTTGAGACCACACTCAAGTCCCGAGCTGTGTCCGAAGACAGTGATG TCAGGTTCACCTGCATTGTCACAGGATACCCAGAGCCAGAGGTGACCTGGTACAAGGATGACATAGAACT GGACCGTTACTGTGGCTTGCCAAAATACGAGATCACTCACCAAGGCAACCGGCACACCCTGCAGCTGTAC AGGTGTCGGGAAGAAGATGCTGCCATCTACCAGGCCTCTGCCCGGAACACCAAGGGCATCGTGTCTTGCT CAGGGGTCCTAGAGGTGGGCACTATGACGGAGTACAAGATTCACCAGCGCTGGTTCGCCAAGTTGAAGCG TAAGGCTGCGGCCAAGATGAGGGAGATTGAGCAGAGCTGGAAGCACGGGAAAGAGGCTTCAGGGGAGGCT GACACACTTCGCAAGATCAGCCCGGACCGCTTCCAAAGAAAGCGCCGACTGAGTGGAGCGGAAGAGGCAG TCCTCCCGGCGCCACTCAGGGGAACGGAGGAAGGGCCCTCAGCAGCTTGGCAGGAAGGAGAGGCTGAGTC CACTCAGCACCCAGGTTTGGGGATGATCAACAGTTTCGCTCCTGGTGAGGCACCCACCAATGGGGAGCCT GCCCCAGAGAACGGGGAGGACGGAGAGCACGGCCTGCTGACCTACATCTGTGAGGTCATGGAACTGGGGC CTCAGAACACCCCTCCAAAGGAGTCTGGGGCTAAGAAGAAAAGGAAGGATGAGGAATCTAAACCAGGAGG GCAGAAACCAGAGTTAGGGAAGGCAGAAGGGAGCCAGTGCTCTTCAGAAAATGGCATCCCTAGCTCAGAC AAACTCAATTCCAGTAGAAGGGAGAAGAAGTCCAGGGATGTGCAGCCAACTCAGAACCAGACCAGAGACA GGGTAGCACGGGGGCCTGGGCCTTCTGGGATAGAAAGCACCAGGAAGACAGCCTCTGTTCTGGGTACTCA AGACAAGGTCCAGGATGTCCCGGCCCTGGCCCCGGCCCCCGCCCCGGCCCCCGCTCCTGTCCCAGTCCCT GCACCCGCCCCTGTTCCAAGCCTCAGCTCAGAGCAGGTGTATTTCTCGCTGAAGGACATGTTCATGGAGA GCACCCGGGCAGGCAGGTCCCAGGAAGAGGAAAAATCTCCACCTCCAAGTACCAGAGTAGCTGGAGAAAG TCCTTCAGGGAAGGTACCTGTCAAGGCTAGAGGGGAGAAGGTACCTATGGTCTCTGGCCAGCCCACGTCT TCCACGGTGCCCCCGCCTATTAAACCTTTGAACAGGAAGAGATTTGCCCCTCCTAAATCCAAAGTGGAGT CAACTACCTCCTCTTCCTCAAGTCAGACTCCAGAATCTGTGGCCCAGAGCTTAGGGAAGGCCCCACCGCC AGCCTCTGCCCAGGTCCCAACACCCCCTGCCCGACGGAGACATGGCACTCGAGAGAGCCCCTTGCAAGGA CAAACAAGCCACAAGACTCCAGGAGAGGCTCTGGAGTCCCCAGCAACCATGGCCCCTGCCACGTCTGCCA GCAGCAGTTCCGATACCATCTCTGTTGGTCACAGCACCTCTGGGAATCAAGGTGTCACGGAGCCCATGGA TACAGAAACTCAGGAAGAGGGGAGGACACTTGCCAATGGGAGAACCAGTGGCAGGCAGAAAACACAGACA GACGGAAAGTTGCAAGTGGATGGGAGGACTCAGGGAGACGAAGCACAAACTTTGCAGAGGACACAGACAA ATGAGAAGGCACCGATGAACGTTGTAACACAAGGAAGCGAAAGGCCAGAGTCAGACAGGAGTTCACAGAA GGATTTGGTGACACAGAGAAGGGTGGATACACAGGTAGGACAGACACAGGCAGGTGAGAGGTGGCAGCAA GACCCGAGAGACGCAAGGATACAGGAGGAAAAGGAGACACAGTCAGTGGGCAGCATTCCCACAGCTTTGG AAACCCAACCAGAACAACCGTCTGTGGTCAGTCTCAGCTCAGTACCAGGAGCCCTCAAACTCTCGCCGTC AGAGTGCCCTACAGAGCCTCAGGCTACAGAATGTTCTGAGAGGAGCACAGAAGCATCTTACATCCAAGAA AAGTCAGGCTTGATGCTTGGATCTGAAGAGGCAGACTTCAGAAGCCATGAGAACACTCTGCTAGGGCCCC CATCAGGGAACCATACCTACTCAACACAGTTGCCCACTGAGGGCAACTCAGAGCATCTGGTAGGAGAAAC ACACCAAAGGCCAGAGTGGTTAGGTGAGGCCAAGCAGGAGGACAGCCTGCTTCAGAGTCCCAAGAAGGAG CAGCTCCAGGAGACGCTGTATGTGGATCTCGCTATTGGACATTCAGCGGACTTGAGTCAGGAGGTGCCCA GTCTGCCTTCTCTTCCTGGAACTGACCTAACCAATAGTCTGCAGGAGGGGCTACCCAGCACCACAGCTTC TCAGCACACAAACGCTGATGTCCCCCTCCCCTCCCCGGACCAGGACCTGCCCAGTTCTGCTCCCACTCTG CAACTGGGACCAGGGTCCCCGACTCAGAGTCACCCACCAGAAGTCATGGCTACTAGCAGTGAGGGAGCCT GTGCCAAGGAGCCAGATGTGGACGGGAGGTCCTCAGGTACCCGGAGCTGTGACCCTGGCCTTATAGACTC CCTGAAGAACTACTTGCTCCTGCTGCTAAAGCTATCCAGTCCAGAGGCAGGCGAAGCCAGGGCAGAGTCC CAGGAAGTGCCTGCCACCGGAGGCTTAACTTCCTCCTCTGCTCTGGTCCCCACCGTGGAAGTGGCTGGCT TGAGTCCCAGGACGTCCAGGCGCATCCTGGACCGTGTGGAGAACAACCACTTGGTGCAGAGCGCACAGAC CCTGCTGCTGAGCCCCTGCACCTCCCGCCGCCTCACTGGCCTCCTGGACCGTGAGGTTCAGGCTGGCCAG CAGGCCCTGGCTGCCGCCCAGTGCTCCCGGGGCCCATGCCCTAGCCCCAGCCCCCTCACCATCCCTGCCA TTGTGGTGGGTGAGGAAGGATCTGCTACTGGTGAGGGAGAGGATTCCAGGGAGAGGACCTCCCAGGAAAG TGACAAGGCTGGACTGCTAGGGGAGGTGGACGGACAAACAGTAGAGAGCAGAACCCAGGAGCCCTGCCAA GAAGAAGCAATCCCAGGGGAGGCTTTGACAGGTCTCCCTGCAGCTACACCTGAGGAACTGGCTCTAGGGG CCCGGAGGAAGAGGTTCCTCCCTAAGGTGAGAGCAGGGTCAGACGGTGAGGTGAACAAGGCTGAAGAAAG AGAGAGTCCCACAGTCTCCCCCCGGGGACCCAGGAAGGGTCTGGCACCTGGGTCACCCGGGACTCCAGGG CGGGAGAGACGGTCCCCTACCCAGGCCCGGAAGGCCAGCATGTTAGAGGTGCCTGGGACAGAAGAAGAGC CTGCATCGGGAGACTTGGTCACCAGACCCAAGGCCAGTGGTCTGGAATCAGAGCCTGCGGTGGATGAAGG CAAGCAGGAAGCACTGGCTAAGCCAAGGAAAGCTAAAGACCTGTTAAAAGCCCCACAGGTGATCCGGAAA ATTCGGGTAGAACAGTTTCCAGATTCTTCTGGTAGTCTGAAGCTTTGGTGTCAGTTTTTCAATATCGTTA GCGACTCAGTCTTGACATGGGCGAAGGATCAGCATCTAGTGGGCGAAGTGAGCAGGAGTGCAGGGGACGA GGGGCCAGCGGCCTTGGCCATTGTGCAGGCGTCACCTACGGACTGTGGCCTGTATCGCTGTACCATCCAA AACGAGCATGGCTCCGCTTCCACTGACTTCCGCCTCAGCCCGGAAGTATTGTCTGGCTTCATCTCCAGAG AGGAAGGTGAAGTTGGAGAAGAGATTGAGATGACCCCCATGGTATTTGCTAAGGGTCTGGCTGACTCTGG CTGCTGGGGGGACAAGCTCTTTGGGCGATTGGTGAGCGAGGAGCTTCGAGGGGGTGGACATGGCCTTCAG AAGGCTTCGCGGGCCAAGGTCATCTATGGGCTGGAGCCCATCTTCGAATCTGGTCGCACGTGCATCATCA AGGTATCCAGCCTGCTCGTGTTCGGACCCAGCAGTGAGACTTCTCTTCTGGGCAGAAACTATGACGTCAC TATCCAGGGATGCAAGATCCAGAACATGAGTCGAGAGTACTGCAAAATCTTTGCAGCTGAAACCCGGGCA GCCTCTGGCTTCGGGGAGGTTCCTGAGATCATCCCACTTTACTTGATCTATCGGCCTGCAAACAATATAC CGTATGCTACCCTGGAGGAAGATCTGGGCAAGCCCCTGCAGACTTACTGTTCCAGGCAGTGGAGCTGTGC TGGGGCCCCAGCAGCATCCAGCAGCTTGGAGCCTGTGCAGAAATGCCAAACCTTCCAGCACTGGCTGTAT CAGTGGACGAACGGCAGCTTCCTTGTCACAGATCTGGCAGGGGCTGACTGGAAGATGACTGATGTAGAGA TCGCTACCAAACTCCGAGGATACCAAGGCCTCAAGGAGAGCTGTTTTCCTGCCCTGCTGGACCAGTTTGC CTCTTCCCACCAGTGCAACACCTACTGTGAGATGCTGGGGCTGAAACCCCTCAAGGGCCCTGAGGGTGCC CACCCTCAAACCAAGGCCAAAGGCTCCAAAAGTCCATCTGCTGGCAGAAAAGGTTCCCAGCTGAGTCCTC AGCCCCAGAAGAAAGGTCTTCCCAGTCCTCAGGGCTCCAGGAAGAGCGCTCCAAGCTCCAGAGCCACGCC TCAGGCCTCCCAGGAGGCCACTCTTCAGTTACTGGGACAGCCCCCTGCCCAAGAGGGGAGCTCTAAGGCC CAGGGCATGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217679 representing NM_001191895 Red=Cloning site Green=Tags(s) MGSRRALGRGWGLGGRAGTGGDGEDDGPVWTPGPASRSYLLSVRPEASLSSNRLSHPSSGRSTFCSIIAQ LTEETQPLFETTLKSRAVSEDSDVRFTCIVTGYPEPEVTWYKDDIELDRYCGLPKYEITHQGNRHTLQLY RCREEDAAIYQASARNTKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKMREIEQSWKHGKEASGEA DTLRKISPDRFQRKRRLSGAEEAVLPAPLRGTEEGPSAAWQEGEAESTQHPGLGMINSFAPGEAPTNGEP APENGEDGEHGLLTYICEVMELGPQNTPPKESGAKKKRKDEESKPGGQKPELGKAEGSQCSSENGIPSSD KLNSSRREKKSRDVQPTQNQTRDRVARGPGPSGIESTRKTASVLGTQDKVQDVPALAPAPAPAPAPVPVP APAPVPSLSSEQVYFSLKDMFMESTRAGRSQEEEKSPPPSTRVAGESPSGKVPVKARGEKVPMVSGQPTS STVPPPIKPLNRKRFAPPKSKVESTTSSSSSQTPESVAQSLGKAPPPASAQVPTPPARRRHGTRESPLQG QTSHKTPGEALESPATMAPATSASSSSDTISVGHSTSGNQGVTEPMDTETQEEGRTLANGRTSGRQKTQT DGKLQVDGRTQGDEAQTLQRTQTNEKAPMNVVTQGSERPESDRSSQKDLVTQRRVDTQVGQTQAGERWQQ DPRDARIQEEKETQSVGSIPTALETQPEQPSVVSLSSVPGALKLSPSECPTEPQATECSERSTEASYIQE KSGLMLGSEEADFRSHENTLLGPPSGNHTYSTQLPTEGNSEHLVGETHQRPEWLGEAKQEDSLLQSPKKE QLQETLYVDLAIGHSADLSQEVPSLPSLPGTDLTNSLQEGLPSTTASQHTNADVPLPSPDQDLPSSAPTL QLGPGSPTQSHPPEVMATSSEGACAKEPDVDGRSSGTRSCDPGLIDSLKNYLLLLLKLSSPEAGEARAES QEVPATGGLTSSSALVPTVEVAGLSPRTSRRILDRVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAGQ QALAAAQCSRGPCPSPSPLTIPAIVVGEEGSATGEGEDSRERTSQESDKAGLLGEVDGQTVESRTQEPCQ EEAIPGEALTGLPAATPEELALGARRKRFLPKVRAGSDGEVNKAEERESPTVSPRGPRKGLAPGSPGTPG RERRSPTQARKASMLEVPGTEEEPASGDLVTRPKASGLESEPAVDEGKQEALAKPRKAKDLLKAPQVIRK IRVEQFPDSSGSLKLWCQFFNIVSDSVLTWAKDQHLVGEVSRSAGDEGPAALAIVQASPTDCGLYRCTIQ NEHGSASTDFRLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVSEELRGGGHGLQ KASRAKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLLGRNYDVTIQGCKIQNMSREYCKIFAAETRA ASGFGEVPEIIPLYLIYRPANNIPYATLEEDLGKPLQTYCSRQWSCAGAPAASSSLEPVQKCQTFQHWLY QWTNGSFLVTDLAGADWKMTDVEIATKLRGYQGLKESCFPALLDQFASSHQCNTYCEMLGLKPLKGPEGA HPQTKAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGSRKSAPSSRATPQASQEATLQLLGQPPAQEGSSKA QGMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191895
ORF Size	5052 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191895.1, NP_001178824.1
RefSeq Size	5055 bp
RefSeq ORF	5055 bp
Locus ID	365298
MW	180.2 kDa

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