Ralgapa1 (NM_020083) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Garnl1; GRIPE; p240; Tulip1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217729 representing NM_020083 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGACTCTATTGACCTTAAACAGTTCTT TGACCAGCATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCCAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG GATAACTGTAGCAAAGAGCAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTTTCAGCACCACAATCTG AATATGGACCTCGAACTTTAGATAATCTTATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTAACTAT AGAGGAAGTCACCCCTCTTGTTCCCCCGCAGTCAGGAGACAAAGGACAAGAAGACCTCACAAGCTATTTT CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACCTCTG CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGGTCAGACCAAAGCCTCATTATGTGATG GTAAAGAAGGATGCTGAAACCAATGAAACGATATACTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTCA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCGCATTCAGGACCTAATATTCCTGGGAT GGAGGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTCTCCAGAGAAGAAAGCAAA AATGATACTGTTGATAAAGCAGACAAAACCGCAGAGCCCGAGCAGTCGCATTCCAATACAAGCACTCTCA CAGAGCGAGAGCCCAGCTCTTCCAGTCTCTGTAGCATCGATGAAGAACACCTCACGGACATTGAAATCGT TCGCAGGGTATTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTGTATCAAGAGTGGATTCAACAGGAGG GAAAACCTTTGTTTATGCAAGAGCCTGAAGAAACTGTGATCACTTCTTCAGATATACCTTGCAGTGAAAA TGTCACAGACCATGACATTTCAATAGAAGACGGAGAGAAGAGAGAAGAGGAAAATGGGACCAATATTTCT GAACATGTTCGAAATTCTACCTGGACAAAGAATGGTTCCTACCAGGAAGCTTTTCATGTCTCTGAAGAAG CCACAGAACAAAATATTCAAGCTGGTACTCAGGCTGTTTTACAGGTGTTCATTATAAACTCATCAAACAT ATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAGCACACAGATATGTGTAAACGTATT CTTAACATATATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTACTCG TGTTGCTCAGAGTCACGGAGTCTGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA AAACATGACCCTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACTCTCATTGTTGCCTGGATCAAAGCA AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCAGTATTGTCATCGTTGACCTATT GGGAAGAATTGGCCACTGAGTGGTCGCTGACCATGGAGACACTAACTAAAGTTTTAGCTAGGAATTTATA TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA GGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC CTGGCCAGGCACCCATGAGACAGAGAAGTGCAACGACTACTGGCTCCCCGGGAACTGAGAAGGCCAGGAG TATAGTACGGCAAAAAACTGTTGATATTGATGATTCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT TTTTCACAGAGTGAAGACACTGGTAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGCGGCAGCAGCGCTAATGTTCCTGACCTGATGGATGAG TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGAAGTAGTCCAGGCAGCC TTGAAATTCCGAAAGACCTTCCTGATATTCTAAACAAGCAGAATCAGATGCGCCCTGTTGATGACCCTGG TGTGCCCTCAGAATGGACGTCTCCTGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG GATTCCTTCAGTGCCTTCCAGTGTGAGGGCCGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA TCCCATCTTCTGCTGATGTGGATTCAGGCTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCTAG CCTAACGACGCTTCATTTAGACTCAGAGACAAGCAGCCTTAATCAACAAGCATTCTCTGCTGAAGTTGCA ACTGTCACTGGTTCAGAAAGTGCTTCCCCTGTTCATTCAGCTCTGGGGTCCAGGTCACAGACACCTTCCC CTTCTACGCTGAATATAGATCACATGGAACAGAAGGATCTACAGCTTGATGAGAAGCTCCACCACTCTGT TCTTCAGACGCCAGATGACCTAGAAATCAGTGAATTTCCTTCTGAATGTTGTAGTGTGATGGCAGGAGGT ACGCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGAAGAATGCTTGGCATTTTGGGAGATG TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT AGCCAAGATCAGAGATAATCTTGGCATTTCAGCCGATAACCTGACTTCACCTTCTCCACCTGTTTTGATT CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCCCCAAATAGAGATTT TTTAACGCATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATCGTCAATACGATC ATCAAGCACTGCTCACCTCAGTTTTTTTCACTTGGTTTGCCTGGGGCCACAATGCTTATTATGGATTTTA TTATAGCGGCTGGGAGAGTGGCTTCTTCAGCTTTTCTTAATGCACCAAGAGTGGAAGCACAGGTTCTTCT GGGATCTTTGGTTTGCTTCCCCAACTTATACTGTGAGCTGCCTGCTCTGCATCCAAGCACGCCTGACATC GCTGTGTCTCAGTTCACAGACGTCAAGGAACTTATAATTAAAACTGTCTTAAGCTCGGCAAGAGACGAAC CCTCTGGCCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATATGGATTTGTGAAGAATTAGTCCACGA ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA GTTGCCCATGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGCACCTAGACTTCAGACTTACCAGC CTGACTCTCCCTTGAAAATTATTCAGATCCTAATAGCAACTATCACACACCTTTTGCCAAGTACAGAAGC TTCATCATATGAAATGGACAAGAGGTTGGTTGTGTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC TTACCTTTAAAGACAATACTCCAATCAGTCCATTCTACAGGAGCAGAAAATGAGAAGACAGAAAAATCTG TCCTCAACTGTATTTATAAGGTTTTACATGGGTGTGTATATGGAGCACAAAGTTTTAGCCATCCAAAGTA CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGACCCTTTTATGCATTTGGAAAGTCTGAGA GAGCCTGAACCTCTACACTCTCCTGATTCAGAACGGTCTTCTAAACTGCAGCCGGTGACAGAAGTGAAAA CTCAGATGCAACAAGGATTAATTTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACCTGGG CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACGAGTCAGGTGTGTGAGAATCATGACAATCATTAT AGCGAAAGTACTGAACTTTCTCCTGAGCTCTTTGACAGTCCAAATATTCAGTTCTTTGTGCTGAATAATA CAACCTTAGTGTCTTGTATCCAGATAAGATCAGAAGAGAGTGTGCCTGGAGGAGGTTTAGCTGCTGGCCT TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC ATACTGTATGGGCCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTTACC AAGAGAAACCAGAAGAGCCTCCTACATCTAATGAATGCTTAGAAGACATAACAGTAAAAGATGGACTTTC TCTCCAGCTTAGAAGATTTAGAGAAACTGTTCCAACTTGGTCTACAATAAGAGAGGAGGAAGATGTTCTT GATGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAATGTTTACAGAGAACTGGAATCTCCCTCAATG TTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA ATACACAGAAGAAAAAGAGTTTGTAGAAAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT GAACCAACACCTCAGAAACCTCAGTCAGCATTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA ATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTGCTCAGAGAACTGAGGAA CTTGGATTCAAGGCAGTGCCGAGAAACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTTGCTGGCCTTGGTTGGG AGGTAAACCTTACAAATCACTGTGGCTTCATGGGAGGACTGCAGAAGAACAAAAGCACTGGACTGACCAC TCCATATTTTGCTACCTCTACAGTAGAAGTAATATTTCATGTATCTACAAGAATGCCTTCTGAATCTGAT GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTACACATTGTTTGGTCTGAACATACTA GAGACTACAGGAGAGGAATTATTCCTACAGAGTTTGGTGATGTCCTCATTGTAATATATCCAATGAAAAA CCACATGTTCAGTATCCAGATCATGAAAAAGCCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCT ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT CACTGATTCCATTGTATCAAAACTTCTACGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA CTTAGAGCCAACGACATTTGAAGACTTTGCAGCCCAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT GCTGATGCAGATCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217729 representing NM_020083 Red=Cloning site Green=Tags(s) MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENADSIDLKQFFDQHFSHIYYVFFENFVTIEASLK QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ DNCSKEQLWMFSCLIPGFSAPQSEYGPRTLDNLINPPLNLQETQVTIEEVTPLVPPQSGDKGQEDLTSYF LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKFYLPYIFPNLCKENSLYHPVLDIPQVRPKPHYVM VKKDAETNETIYCTKEPFIQARVIVIRWLVSFWLEPKPHSGPNIPGMEGEVLPKNIQRAAASLVSREESK NDTVDKADKTAEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL LPICEAAAMRKVVKVYQEWIQQEGKPLFMQEPEETVITSSDIPCSENVTDHDISIEDGEKREEENGTNIS EHVRNSTWTKNGSYQEAFHVSEEATEQNIQAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRI LNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMSSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIKA NLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKGK GVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDSQILPRSTRVRH FSQSEDTGNEVFGALHEEQPLPRSSSTSDILEPFTVERAKVNKEDTSPKLPPLNSETGGSSANVPDLMDE FIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPVDDPGVPSEWTSPASAGSSDLMSSDSHS DSFSAFQCEGRKFDNFGFGTDIGIPSSADVDSGSGHHQSTEEQEVASLTTLHLDSETSSLNQQAFSAEVA TVTGSESASPVHSALGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAGG TLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLI PPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTI IKHCSPQFFSLGLPGATMLIMDFIIAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSTPDI AVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKT VAHVACNMLHMLVHYAPRLQTYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMA LPLKTILQSVHSTGAENEKTEKSVLNCIYKVLHGCVYGAQSFSHPKYFPISLSDLASVDYDPFMHLESLR EPEPLHSPDSERSSKLQPVTEVKTQMQQGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHY SESTELSPELFDSPNIQFFVLNNTTLVSCIQIRSEESVPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSA ILYGPPIVSGLPEPTSFILSMSYQEKPEEPPTSNECLEDITVKDGLSLQLRRFRETVPTWSTIREEEDVL DELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQD EPTPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQED KYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESD DSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGA IVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHFP ADADH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_020083
ORF Size	6105 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020083.3, NP_064468.3
RefSeq Size	7476 bp
RefSeq ORF	6108 bp
Locus ID	56785
UniProt ID	O55007
MW	229.5 kDa
Gene Summary	mouse homolog interacts with the HLH region of E12 and may be involved in neuronal differentiation [RGD, Feb 2006]

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