Sorl1 (NM_053519) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Lr11; RGD619914
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217754 representing NM_053519 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACACGGAGCAGCAGGAGGGAGTCGAGACTCCCTTTCCTATTCACCCTGGTCGCTCTGCTGCCCC CCGGGGCTCTCGGCGGGGGCTGGACGCAGAGATTGCATGGTGGCGGAGCGCCCTTGCCCCAGGACCGCGG CTTCTTCGTGGTGCAGGGCGACCCGCACGAGCTGCGGCTGGGAACGCACGGGGATGCCTGGGGGGCGAGC CCAGCGGCCAGGAAGCCACTCAGGACGCGGCGGAGCGCTGCTTTGCAGCCCCAGCCTATCCAGGTGTATG GACAGGTCAGCCTGAATGACTCCCACAATCAGATGGTGGTGCACTGGGCTGGAGAGAAAAGCAACGTGAT CGTGGCATTAGCCCGAGACAGCCTGGCATTGACAAGGCCCAAGAGCAGTGATGTATATGTGTCTTATGAC TATGGAAAATCATTCAATAAGATTTCAGAGAAGCTGAACTTTGGTGTGGGAAACAGCAGTGAAGCCGTGA TCTCCCAATTCTACCACAGCCCTGCCGACAACAAGAGGTACATCTTTGTGGATGCTTATGCCCGATACCT CTGGATCACGTTTGACTTCGGCAGCACGATCCATGGCTTCTCCATCCCATTCCGGGCGGCTGATCTCCTC CTTCATAGCAAGGCCTCCAACCTCCTCTTGGGCTTTGACAGCTCTCACCCCAACAAGCAGCTATGGAAGT CGGATGATTTTGGCCAGACCTGGATCATGATTCAGGAACATGTGAAGTCCTTTTCTTGGGGGATCGACCC CTATGACCAGCCGAACACTATCTACATCGAGCGGCATGAACCCTTTGGGTTCTCCACAGTGTTCCGCAGC ACAGACTTCTTCCAGTCCCGGGAAAACCAGGAAGTGATCCTGGAGGAAGTGAGAGACTTTCAGCTTCGGG ACAAGTACCTGTTTGCTACAAAGGTGGTGCATCTTCCGGGCAGCCAGCAGCAGTCATCTGTCCAGCTCTG GGTCTCCTTTGGCCGGAAGCCTATGCGAGCAGCCCAGTTTGTCACAAAGCATCCTATTAACGAATACTAC ATCGCCGATGCCTCCGAAGACCAGGTCTTCGTGTGCGTCAGCCACAGCAACAATAGTACTAACCTGTACA TCTCAGAGGCGGAGGGGCTGAAATTCTCCCTATCCTTGGAGAATGTGCTGTATTATAGCCCAGGAGGGGC CGGCAGTGACACCCTGGTGAGGTATTTTGCAAACGAGCCGTTTGCCGACTTTCACCGAGTGGAGGGCTTA CAAGGAGTCTACATTGCCACCCTGATTAATGGATCTATGAGTGAGGAGAACATGAGGTCGGTCATCACCT TTGACAAAGGGGGAACCTGGGAATTTCTTCAGGCCCCGGCCTTCACAGGATATGGAGAGAAAATCAATTG TGAGCTCTCTCAGGGCTGCTCCCTCCACCTGGCCCAGCGCCTCAGTCAACTGCTCAACCTCCAACTTCGG AGGATGCCGATCCTGTCCAAGGAGTCAGCCCCCGGCCTCATCATCGCCACCGGCTCTGTGGGAAAGAACT TGGCCAGCAAGACCAACGTGTACATCTCTAGCAGTGCCGGAGCCCGGTGGAGAGAGGCTCTTCCAGGACC TCATTACTATACTTGGGGTGACCATGGAGGCATCATCATGGCCATTGCCCAGGGCATGGAGACCAACGAG CTGAAGTACAGCACCAATGAAGGCGAGACCTGGAAGACGTTCGTCTTCTCTGAGAAGCCTGTGTTTGTGT ATGGGCTTCTCACGGAACCTGGCGAGAAGAGCACCGTCTTCACCATCTTCGGCTCTAACAAGGAGAGTGT CCACAGCTGGCTCATCCTGCAGGTCAATGCCACCGACGCCTTGGGGGTTCCTTGCACGGAAAACGACTAC AAGCTGTGGTCCCCATCTGATGAGCGGGGGAACGAGTGTTTGCTAGGACACAAGACAGTTTTCAAAAGGA GGACCCCTCACGCAACGTGCTTTAATGGGGAAGACTTTGACCGGCCGGTGGTCGTGTCCAACTGCTCCTG CACCCGGGAGGACTACGAATGTGACTTTGGCTTCAAGATGAGTGAAGATATCTCCTTAGAGGTTTGTGTT CCAGACCCTGAGTTTTCTGGGAAGCCCTACAGCCCTCCTGTGCCTTGTCCCGTGGGTTCTTCCTACAGGA GAACAAGAGGCTATCGGAAGATTTCTGGGGATACGTGCAGTGGAGGAGATGTTGAGGCACGGCTGGAGGG GGAGCTGGTCCCCTGTCCCCTGGCAGAAGAGAATGAGTTCATCCTGTACGCCATGCGGAAATCCATCTAC CGCTACGACTTGGCCTCGGGAGCCACGGAACAGCTTCCTCTCTCGGGGCTGCGGGCAGCCGTGGCCCTGG ACTTTGACTATGAGCACAACTGCCTGTATTGGTCTGACCTGGCGCTGGACACCATCCAGCGTCTCTGCTT GAATGGGAGCACAGGGCAAGAGGTGATCATCAATTCTGGCCTGGAGACCGTGGAGGCTTTGGCCTTTGAA CCCCTCAGCCAGTTACTTTACTGGGTGGATGCCGGCTTTAAAAAGATCGAGGTAGCTAATCCAGATGGTG ACTTCCGGCTGACAATCGTCAATTCCTCTGTGCTTGATCGACCCAGGGCTCTAGTCCTTGTGCCCCAAGA GGGGGTGATGTTCTGGACTGACTGGGGAGACTTGAAGCCTGGAATTTACCGGAGCAACATGGATGGGTCT GCTGCCTATCGCCTTGTGTCAGAGGACGTGAAGTGGCCTAATGGCATCTCTGTGGACGGCCACTGGATTT ACTGGACGGACGCTTACCTGGACTGTATCGAGCGCATCACTTTCAGTGGCCAGCAGCGTTCAGTCATCCT TGACAACCTCCCACACCCTTACGCCATTGCCGTCTTTAAGAACGAGATCTACTGGGACGACTGGTCGCAG CTCAGTATCTTCAGAGCTTCCAAGTACAGCAGGTCCCAGGTGGAGACCCTAGCGAGCCAGCTCACCGGCC TGATGGACATGAAGATCTTCTACAAAGGAAAGAACGCCGGAAGCAATGCCTGCATTCCTCAGCCCTGCAG TCTACTGTGCCTCCCTAAGGCCAACAACAGCAAAAGCTGCAGGTGTCCAGAAGGCGTGGCCAGCAGTGTC CTCCCCTCTGGGTACCTGATGTGTGACTGCCCTCAGGGCTACGAGAGGAAGAACAACACCTGTGTCAAAG AAGAGAACACCTGTCTTCGCAACCAGTACCACTGCAGCAACGGCAACTGTATCAACAGCATTTGGTGGTG TGATTTCGACAACGACTGTGGCGATATGAGTGACGAGAGGAATTGCCCCACGACCATCTGTGATGCAGAC ACTCAGTTCCGGTGCCAGGAGTCGGGGACTTGTATTCCACTCTCCTATAAGTGTGACCTTGAAGATGACT GTGGAGATAACAGTGATGAGAGTCACTGTGAGATGCACCAGTGTCGCAGCGACGAGTTCAACTGTAGCTC TGGGATGTGCATCCGGTCCTCCTGGGTGTGCGACGGAGACAACGACTGTCGGGACTGGTCTGATGAAGCC AACTGCACCGCCATCTACCACACCTGTGAGGCCTCCAACTTCCAATGCCACAATGGCCACTGCATCCCCC AGCGATGGGCATGTGACGGTGACGCGGATTGCCAGGACGGCTCTGATGAAGATCCCATCAGCTGTGAGAA AAAGTGCAATGGATTCCACTGCCCAAACGGCACGTGCATCCCATCCAGCAAGCATTGTGACGGGCTGCGG GACTGCCCCGACGGCTCTGACGAGCAGCACTGTGAGCCCTTCTGCACACGTTTCATGGACTTTGTGTGTA AGAACCGTCAGCAGTGTCTGTTCCACTCCATGGTGTGTGACGGGATCATCCAGTGCCGAGATGGCTCTGA TGAGGATGCCACCTTCGCCGGATGCTCCCAAGACCCGGAGTTCCACAAGGAGTGTGACGAGTTCGGCTTC CAGTGTCAGAACGGAGTGTGCATCAGCTTGATTTGGAAATGCGATGGGATGGATGACTGCGGAGACTACT CTGACGAGGCCAACTGTGAAAACCCCACAGAAGCCCCGAACTGCTCCCGCTACTTCCAGTTCCACTGTGA GAATGGCCGCTGCATCCCCAACAGGTGGAAATGTGACAGAGAGAATGACTGCGGGGACTGGTCTGATGAG AAGGACTGTGGAGATTCACACATTTTCCCCTCCCCCACCCCCGGACCGTCCACATGTCTGCCCAATTACT TCCGATGCAGCAGCGGGGCCTGCGTGATGGGCACCTGGGTGTGCGACGGCTACAGGGACTGTGCTGATGG CTCAGATGAGGAAGCCTGCCCCTCTCTAGCAAACTCCACTGCCGCCTCCACTCCCACCCAGCTTGGGCAG TGTGACCGATTTGAGTTCGAGTGCCGCCAACCAAAGAAATGTATCCCCAACTGGAAGCGCTGTGATGGCC ACCAGGATTGCCAAGACGGCCAGGACGAAGCCAACTGCCCCACTCACAGCACCTTGACGTGTATGAGCAG GGAGTTCAAGTGTGAGGACGGCGAGGCGTGCATTGTGCTCTCGGAGCGCTGTGACGGCTTCCTGGATTGC TCTGATGAGAGTGATGAGAAGGCCTGCAGCGATGAGTTAACAGTATATAAAGTCCAGAATCTTCAATGGA TAGCTGATTTCTCTGGGGATGTGACTTTGACCTGGACGCGACCAAAAAAAATGCCATCTGCTTCTTGTGT ATACAATGTGTACTACAGGGTGGTTGGAGAGAGCATATGGAAGACCCTGGAAACCCATAGCAATAAAACA AGCACTGTGCTGAAGGTCCTGAAGCCAGACACCACGTACCAGGTTAAGGTCCAGGTGCACTGCCTTAGCA AGGTGCACAACACCAATGACTTTGTGACCCTGAGGACTCCAGAGGGGTTGCCGGATGCCCCTCGGAATCT CCAGCTCTCCCTAAACAGTGAAGAGGAAGGTGTGATTCTTGGGCACTGGGCTCCTCCCGTCCACACCCAT GGCCTCATCCGTGAGTACATTGTGGAATACAGCCGGAGTGGATCCAAGATGTGGGCCTCCCAGAGAGCTG CCAGCAACTCTACAGAGATAAAGAACTTACTGCTCAATGCCCTCTACACCGTCCGAGTGGCTGCGGTGAC TAGTCGGGGAATAGGAAACTGGAGTGATTCCAAATCCATCACCACCACAAAAGGAAAAGTGATCCAAGCA CCAAATATCCACATTGACAGCTACGATGAAAATTCTCTCAGCTTTACCCTGACCATGGACGGTGACATCA AGGTCAATGGCTATGTGGTGAACCTTTTCTGGTCATTTGATGCCCACAAACAAGAAAAGAAAACCTTGAA CTTCCGAGGAGGAAGCTCGCTGTCACACAAAGTGAGCAATCTGACAGCTCATACGTCCTATGAGGTTTCT GCCTGGGCCAAGACAGACTTGGGGGACAGTCCTCTGGCCTTTGAGCACATCTTGACCAGAGGCATCAGTC CACCTGCTCCTAGTCTCAAAGCCAAGGCCATCAATCAGACTGCAGTGGAGTGCATCTGGACTGGCCCCAA GAATGTGGTGTATGGTATTTTCTACGCCACATCCTTCCTCGACCTCTACCGAAACCCTAAGAGTTTAACT ACTTCACTCCACAATAAGACAGTCATTGTCAGCAAGGATGAGCAGTATTTGTTTCTGGTCCGGGTGTTGA TCCCTTACCAAGGGCCGTCCTCCGACTACGTTGTGGTGAAGATGATCCCAGACAGCAGGCTTCCACCTCG GCACCTACATGCCGTCCAAATAGGCAAGACCTCAGCAGTCATCAAGTGGGAGTCACCCTACGACTCTCCT GACCAGGACCTGTTCTACGCCATTGCAGTGAAAGATCTGATCCGAAAGACGGACAGGAGCTACAAAGTCA GATCCCGCAACAGTACTGTGGAGTACAGCCTTAGCAAGCTGGAGCCTGGAGGGAAATACCATATCATTGT CCAGCTGGGAAACATGAGCAAGGACTCCAGCATAAAAATCACCACCGTCTCATTGTCAGCACCTGATGCC TTAAAAATCATAACCGAAAACGACCACGTTCTCCTCTTTTGGAAAAGTCTAGCTTTAAAGGAAAAGCAGT TCAATGAAACCAGGGGCTACGAGATACACATGTTTGACAGTGCCGTGAATCTCACCGCTTACCTTGGGAA TACTACTGACAATTTCTTTAAAGTGTCCAACCTGAAGATGGGTCACAATTACACTTTCACGGTCCAGGCT CGATGTCTCTTTGGTAGCCAGATCTGCGGGGAGCCTGCCGTGCTGCTGTATGACGAACTGAGCTCAGGTG GAGATGCCACAGTGGTCCAGACTGCCAGGTCTACTGACGTCGCGGCCGTGGTGGTTCCTATCTTGTTCCT GATCCTGCTGAGCCTGGGAGTCGGCTTTGCCGTCCTCTACACGAAGCACCGCCGCTTACAAAGCAGCTTC ACTGCCTTTGCCAACAGCCACTACAGCTCCAGGCTGGGCTCCGCCATCTTCTCTTCCGGGGATGACCTAG GAGAGGACGACGAAGATGCTCCCATGATTACCGGATTCTCAGACGATGTCCCCATGGTGATAGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217754 representing NM_053519 Red=Cloning site Green=Tags(s) MATRSSRRESRLPFLFTLVALLPPGALGGGWTQRLHGGGAPLPQDRGFFVVQGDPHELRLGTHGDAWGAS PAARKPLRTRRSAALQPQPIQVYGQVSLNDSHNQMVVHWAGEKSNVIVALARDSLALTRPKSSDVYVSYD YGKSFNKISEKLNFGVGNSSEAVISQFYHSPADNKRYIFVDAYARYLWITFDFGSTIHGFSIPFRAADLL LHSKASNLLLGFDSSHPNKQLWKSDDFGQTWIMIQEHVKSFSWGIDPYDQPNTIYIERHEPFGFSTVFRS TDFFQSRENQEVILEEVRDFQLRDKYLFATKVVHLPGSQQQSSVQLWVSFGRKPMRAAQFVTKHPINEYY IADASEDQVFVCVSHSNNSTNLYISEAEGLKFSLSLENVLYYSPGGAGSDTLVRYFANEPFADFHRVEGL QGVYIATLINGSMSEENMRSVITFDKGGTWEFLQAPAFTGYGEKINCELSQGCSLHLAQRLSQLLNLQLR RMPILSKESAPGLIIATGSVGKNLASKTNVYISSSAGARWREALPGPHYYTWGDHGGIIMAIAQGMETNE LKYSTNEGETWKTFVFSEKPVFVYGLLTEPGEKSTVFTIFGSNKESVHSWLILQVNATDALGVPCTENDY KLWSPSDERGNECLLGHKTVFKRRTPHATCFNGEDFDRPVVVSNCSCTREDYECDFGFKMSEDISLEVCV PDPEFSGKPYSPPVPCPVGSSYRRTRGYRKISGDTCSGGDVEARLEGELVPCPLAEENEFILYAMRKSIY RYDLASGATEQLPLSGLRAAVALDFDYEHNCLYWSDLALDTIQRLCLNGSTGQEVIINSGLETVEALAFE PLSQLLYWVDAGFKKIEVANPDGDFRLTIVNSSVLDRPRALVLVPQEGVMFWTDWGDLKPGIYRSNMDGS AAYRLVSEDVKWPNGISVDGHWIYWTDAYLDCIERITFSGQQRSVILDNLPHPYAIAVFKNEIYWDDWSQ LSIFRASKYSRSQVETLASQLTGLMDMKIFYKGKNAGSNACIPQPCSLLCLPKANNSKSCRCPEGVASSV LPSGYLMCDCPQGYERKNNTCVKEENTCLRNQYHCSNGNCINSIWWCDFDNDCGDMSDERNCPTTICDAD TQFRCQESGTCIPLSYKCDLEDDCGDNSDESHCEMHQCRSDEFNCSSGMCIRSSWVCDGDNDCRDWSDEA NCTAIYHTCEASNFQCHNGHCIPQRWACDGDADCQDGSDEDPISCEKKCNGFHCPNGTCIPSSKHCDGLR DCPDGSDEQHCEPFCTRFMDFVCKNRQQCLFHSMVCDGIIQCRDGSDEDATFAGCSQDPEFHKECDEFGF QCQNGVCISLIWKCDGMDDCGDYSDEANCENPTEAPNCSRYFQFHCENGRCIPNRWKCDRENDCGDWSDE KDCGDSHIFPSPTPGPSTCLPNYFRCSSGACVMGTWVCDGYRDCADGSDEEACPSLANSTAASTPTQLGQ CDRFEFECRQPKKCIPNWKRCDGHQDCQDGQDEANCPTHSTLTCMSREFKCEDGEACIVLSERCDGFLDC SDESDEKACSDELTVYKVQNLQWIADFSGDVTLTWTRPKKMPSASCVYNVYYRVVGESIWKTLETHSNKT STVLKVLKPDTTYQVKVQVHCLSKVHNTNDFVTLRTPEGLPDAPRNLQLSLNSEEEGVILGHWAPPVHTH GLIREYIVEYSRSGSKMWASQRAASNSTEIKNLLLNALYTVRVAAVTSRGIGNWSDSKSITTTKGKVIQA PNIHIDSYDENSLSFTLTMDGDIKVNGYVVNLFWSFDAHKQEKKTLNFRGGSSLSHKVSNLTAHTSYEVS AWAKTDLGDSPLAFEHILTRGISPPAPSLKAKAINQTAVECIWTGPKNVVYGIFYATSFLDLYRNPKSLT TSLHNKTVIVSKDEQYLFLVRVLIPYQGPSSDYVVVKMIPDSRLPPRHLHAVQIGKTSAVIKWESPYDSP DQDLFYAIAVKDLIRKTDRSYKVRSRNSTVEYSLSKLEPGGKYHIIVQLGNMSKDSSIKITTVSLSAPDA LKIITENDHVLLFWKSLALKEKQFNETRGYEIHMFDSAVNLTAYLGNTTDNFFKVSNLKMGHNYTFTVQA RCLFGSQICGEPAVLLYDELSSGGDATVVQTARSTDVAAVVVPILFLILLSLGVGFAVLYTKHRRLQSSF TAFANSHYSSRLGSAIFSSGDDLGEDDEDAPMITGFSDDVPMVIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053519
ORF Size	6645 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053519.1, NP_445971.1
RefSeq Size	10537 bp
RefSeq ORF	6648 bp
Locus ID	300652
UniProt ID	P0DSP1
MW	247.3 kDa
Gene Summary	may play a role in lipoprotein metabolism; increased expression occurs in response to balloon injury endothelial denudation in an experimental model for atherogenesis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.