ASB6 (AK022220) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK022220, the custom clone sequence may differ by one or more nucleotides ATAACAAGCCCTAAATAAAAATAGAGGTGGACTTTGCTCATGGAGTGTTACATCAGTTCTCCCAAAACTA ATTGATAATTTCAGTGCAATCTCTGGTAATTATAGCAGATTTTTTTTTTTGTGGAAATTGAGAAGCTGAA TCTAAAACCGATATGGAAATACAAATGAGTAAGAGTAGCCAAAACAGTAAGTTACTCATCCCAGTATTAA GACTGTGCAGCTACAGTGATGAGAGTGTAGTGCTGGTAAGAGGACTGGCACGCAGGCCAGTGGGGTGGAA TGGAGCCCGGAAAGTGAACCACAAACTTTTGGTTCACAGAGGTACCAGGATAATTCAAGGGGGAGGAATT GTCTTATCTACAGGTGGTTCTGGCAACAGAGTATTCACAGGAAAAATGGTGAACGTTAACCCTTGCATCA TATGCAAAAAATTATTTGAAACAGGTCATAAGAACTAAAACCATCAAACTTCTAGGAGAAAATACAGGGA AAAATCTCTGTGGCCTTGACCATGCAAAAATTTCTTGGGACACAAAAAGCATGAGCCACAAAAGAAAACG TTGATAGGTGGGATCTCATCAAAATTTCAAACTCTCTTTCTTTGAAAGACAGTTAAGAAAATAAAAAGGC AAGCCACGCCCTCCAAAAATACATGCAGTACATATACAGGACAAAGGACTTATTTCTAGAACCTGTAAAG AACTCTTAGAACTCAATAATAAGAAAACAACCCAGTAAAACAATGGGCGAAAGATTTAAACATGCATTTC CCAGAAGGTATATGAGTGGGCATTAAGCACACACAGGGTATCATTATTTATCAGGGACATGCAAATTCAC CCATGAGCTACCTGAACATACTTGCTAGAATGGTCAAAATGAAGAAGAGACAGTCTTCATTGTTGATAAG GATATGGAGCAGTTGAAACGCTCACACGTTATTGATAGGAATGTAAAATGGGGCCGGGTACAGTGGCTCA TGCCTGTAATCCCAGCACTTTGGGACGCCGAGGCAGTCGGATCACTTGAGGTTAGGAGTTTGAGACCAGC CTGGCTAACGTGGCGAAACCTGTCTCTACTAAAAATAGAAAAACTAGCCAGACATGATGGTGAACGCCTA TAATCCCAGCTACTCAGGAGACTGAGGCAGGAGAATCACTGGAGATGGGGAGGATCACTTGAACCCAAGA GGCGGAGGCTACAGTAAGCAGCAGTCGTGCCACTGCACTCCAGCCTGGGGGACAGAATGAAGCTCTGCCA AAAACAAAAATAGGGAATGTAAAATGGTACCATTGTTTTGGAAAACAGTTTGGCAGTTTCTTATTAAGGT AGACGAACACTTCCATGTGACCAAACAATTCAACTTTTATTTCCCCAAGAGAAATAAAAACCTATGTCCA TGCAAAGACTGTTACTACTTTATGTATAATATCAATAAACTGAAAATAACCCTGACTTCCATCAAGAATG AAATGGATATTTGGTATGTTTGTAGCAGTGGAGTGCCACACAGCAGTAAAAATGAATGATTATTAATACA TACAGCAGTATCTATGGATCTCAAAAACAGGGAAAGAGGCCAAACAATGAAGTATGTGCCATACAATTTC ATTTATTTGCAATTCTAGAAGCTAAACTAATCCATAGGGACAGAAGGCATGTCTGTGGTTGCCTGGGGTT GGGTGTGGAGGGAAAGACTCCCTACCAGTGGGTACAAGGGAACTTTCTGGGCATGATGAGAATATTCTGT ATCTTCATTGTGGCTTGATGTTATGTTTGTCAAAACTCATTGAACTCTACACTTCAGATGGGTGCATTTT ACCATATATGTCATCTCTCAATAAACTGAATTTTTTAAAAATTAAAGGAATTTACTTAATTGCAAAAGAG GACTGGAAAGGAAAGGCTGCCGAGCATCTTGGCCTGCTGTACCCCTCTTCCTTCAAAGGAGGGTGTGGCC ACGTCCGACAGCAGAGTTGAATGAAGCCTGCATGCTTGCACAACATCAGAACTGTCAGAATGTCAAGATG GAATACTTTCAATTGAGTTTTACAAAAAGACAAGAAATTTGCTGGGTGTGGTGGCGCATGCCTGTAATCC TAGCACTTTGGGAGGCCAAGGTGGGGGAATTGCTTGTAGTCAGGAGTTTAAGACCATCCCGAGCAGCAAA GTGAGACCCTGTTTTTACTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK022220 unedited NNGGGGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTGAA GCAAGGGNAACCATAGNANAAAACAACATTAGAGTGTCCATAAAACATCCTAAGGAACCC ACAGAGAACCCACTAGAACTAATAAGTAAAGTTAGCAAGGTTGAAGAATTCAAGGTCAGT ATACAAAGACCAATTGTATTTCTGTGTATTAGCAATAAACAATTGGAAAATGAAATTTTA AAAATTCAATTTCATTAGCAATTTCAGTAGCATCTAAAAACTCGAAATATGGAGGAATAA ATTTAAGAAAATATTTGTAGGACATGCACATTGATAACAAGCCCTAAATAAAAATAGAGG TGGACTTTGCTCATGGAGTGTTACATCAGTTCTCCCAAAACTAATTGATAATTTCAGTGC AATCTCTGGTAATTATAGCAGATTTTTTTTTTTGTGGAAATTGAGAAGCTGAATCTAAAA CCGATATGGAAATACAAATGAGTAAGAGTAGCCAAAACAGTAAGTTACTCATCCCAGTAT TAAGACTGTGCAGCTACAGTGATGAGAGTGTAGTGCTGGTAAGAGGACTGGCACGCAGGC CAGTGGGGTGGAATGGAGCCCGGAAAGTGAACCACAAACTTTTGGTTCACAGAGGTACCA GGATAATTCAAGGGGGAGGAATTGTCTTATCTACAGGTGGTTCTGGCAACAGAGTATTCA CAGGAAAAATGGTGAACGTTAACCCTTGCATCATATGCAAAAAATTATTTGAAACAGGTC ATAAGAACTAAAACCATCAAACTTCTANGAGAAAATACAGGGAAAAATCTCTGTGGCCTT GACCATGCANAAAATTCTTGGGACACAAAAAGCATGAGCCACAAAGAAAAACGTGATAGG TGGGATCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK022220 unedited GGTTTCTATTAAGCGGCCGCAATCTAAGATCGATTTTTTTTTTTTTTTTTTAAGTAAAAA CAGGGTCTCACTTTGCTGCTCGGGATGGTCTTAAACTCCTGACTACAAGCAATTCCCCCA CCTTGGCCTCCCAAAGTGCTAGGATTACAGGCATGCGCCACCACACCCAGCAAATTTCTT GTCTTTTTGTAAAACTCAATTGAAAGTATTCCATCTTGACATTCTGACAGTTCTGATGTT GTGCAAGCATGCAGGCTTCATTCAACTCTGCTGTCGGACGTGGCCACACCCTCCTTTGAA GGAAGAGGGGTACAGCAGGCCAAGATGCTCGGCAGCCTTTCCTTTCCAGTCCTCTTTTGC AATTAAGTAAATTCCTTTAATTTTTAAAAAATTCAGTTTATTGAGAGATGACATATATGG TAAAATGCACCCATCTGAAGTGTAGAGTTCAATGAGTTTTGACAAACATAACATCAAGCC ACAATGAAGATACAGAATATTCTCATCATGCCCAGAAAGTTCCCTTGTACCCACTGGTAG GGAGTCTTTCCCTCCACACCCAACCCCAGGCAACCACAGGCATGCCTTCTGTCCCTATGG ATTAGTTTAGCTTCTAGAATTGCAAATAAATGAAATTGTATGGCACATACTTCATTGTTT GGCCTCTTTCCCTGTTTTTGAGATCCATAGATACTGCTGTATGTATTAATAATCATTCAT TTTTACTGCTGTGTGGCACTCCACTGCTACAAACATACCAAATATCCATTTCATTCTTGA TGGAAGTCAGGNTTATTTTCAGTTTATTGATATATACATAAAGTAGTAACAGTCTTTGCA TGGACATAAGTTTTTATTTCTCTTGGGGAAAATAAAGTTGAATTGTTTGGTCACTGGAAN TT
Restriction Sites	NotI-NotI
ACCN	AK022220
Insert Size	2400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK022220.1
RefSeq Size	2190 bp
RefSeq ORF	2190 bp
Locus ID	140459
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene belongs to a family of ankyrin repeat proteins that, along with four other protein families, contain a C-terminal SOCS box motif. Growing evidence suggests that the SOCS box, similar to the F-box, acts as a bridge between specific substrate-binding domains and the more generic proteins that comprise a large family of E3 ubiquitin protein ligases. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]

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