BOLA2 (AF271775) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF271775, the custom clone sequence may differ by one or more nucleotides ATGGTCATTACATATGGATTAGACCAACTGGAGAATTGCCAGACTTGTGGTACCAATTATATCATCTCAG TCTTGAATTTACTCACGCTGATTGTTGAACAGATAAATACGAAACTGCCATCATCATTTGTAGAAAAACT GTTTATACCATCATCTAAACTACTATTCTTGCGTTATCATAAAGAAAAAGAGGTTGTTGCTGTAGCCCAT GCTGTTTATCAAGCAATGCTCAGCTTGAAGAATATTCCTGTTTTGGAGACTGCCTATAAGTTAATATTGG GAGAAATGACTTGTGCCCTAAACAACCTCCTGCACAGTCTGCAACTTCCTGAGGCCTGTTCTGAAATAAA ACATGAGGCTTTTAAGAATCATGTGTTCAATGTAGACAATGCAAAATTTGTAGTTAAATTTGACCTCAGT GCCCTGACTACAATTGGAAATGCCAAAAACTCGAGTCTTTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF271775 unedited AAATTCCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGGCGCCCGAGCTGGGGTTGTGTCCCTGCTGGGCTGCCGTTCCAGC TGGACTGCCGCCATGGAACTCAGCGCCGAATACCTCCGCGAGAAGCTGCAGCGGGACCTG GAGGCGGAGCATGTGGAGGTGGAGGACACGACCCTCAACCGTTGCTCCTGTAGCTTCCGA GTCCTGGTGGTGTCGGCCAAGTTCGAGGGGAAACCGCTGCTTCAGAGACACAGCTTGGAT CCTAGCATGACTATACATTGTGACATGGTCATTACATATGGATTAGACCAACTGGAGAAT TGCCAGACTTGTGGTACCAATTATATCATCTCAGTCTTGAATTTACTCACGCTGATTGTT GAACAGATAAATACGAAACTGCCATCATCATTTGTAGAAAAACTGTTTATACCATCATCT AAACTACTATTCTTGCGTTATCATAAAGAAAAAGAGGTTGTTGCTGTAGCCCATGCTGTT TATCAAGCAATGCTCAGCTTGAAGAATATTCCTGTTTTGGAGACTGCCTATAAGTTAATA TTGGGAGAAATGACTTGTGCCCTAAACAACCTCCTGCACAGTCTGCAACTTCCTGAGGCC TGTTCTGAAATAAACATGAGGCTTTTAAGAATCATGTGTTCAATGTAGACAATGCAACAT TTGTAGTTAAATTTGACCTCAGTGCCCTGACTACTATTGGAAATGCCAAAGACTCGAGTC TTTCATTGTACTGACGTTTGTTTATCCACAGTTAAGCCTTTTCTCATACATATTTATGTA TTCACTGNCATGTCACATGTCTGCAGATCACTGTATGTAGCAACANCCTAT
Restriction Sites	NotI-NotI
ACCN	AF271775
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF271775.1, AAG44786.1
RefSeq Size	1489 bp
RefSeq ORF	462 bp
Locus ID	552900
Gene Summary	This gene is located within a region of a segmental duplication on chromosome 16 and is identical to BOLA2B (bolA family member 2B). The product of this gene belongs to a family of proteins that are widely conserved and may be involved in iron maturation. Related pseudogenes are found multiple different chromosomes. Alternative splicing results in multiple transcript variants. Transcripts initiating at this locus may extend into downstream SMG1 pseudogene 6 (SMG1P6) and encode fusion proteins with a C-terminus related to SMG1 phosphatidylinositol 3-kinase-related kinase. A readthrough locus is represented with GeneID:107282092. [provided by RefSeq, Feb 2016]

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