POLK (NM_016218) Human Untagged Clone

Name: POLK (NM_016218) Human Untagged Clone
SKU: SC111277
Price: 6376.00

CAT＃: SC111277

POLK (untagged)-Human polymerase (DNA directed) kappa (POLK)

Datasheet

SDS

"NM_016218" in other vectors (6)

CNY 6,376.00

货期*

现货

规格

Product images

经常一起买 (4)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA319484

Goat polyclonal anti-POLK antibody

CNY 8,025.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DINB1; DINP; POLQ
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC111277 sequence for NM_016218 edited (data generated by NextGen Sequencing) ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCTTAGGATGGGA CTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATTAACAAAATTATA ATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGAAAGAAAAGCAAGTC AACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCACCAGCCAACAGCTAAGA AAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAACAAAGCCGAAATTTGAGC AATACCATAGTGCACATTGACATGGATGCTTTCTATGCAGCTGTAGAAATGAGGGACAAT CCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAATGAGTATGCTGTCTACTTCAAAT TACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATGCCAGGATTTATTGCTAAGAGGCTG TGCCCACAACTTATAATAGTGCCCCCCAACTTTGACAAATACCGAGCTGTGAGTAAAGAG GTTAAGGAAATACTTGCTGATTATGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCC TACTTGAATATAACAAAGCACTTAGAAGAAAGACAAAATTGGCCTGAGGATAAAAGAAGG TATTTCATCAAAATGGGAAGCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTTAATAAA CTGAGTGAGCATGAACGATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTG CAGCCCCCAGGAGATCCTTTCCAAGTGAACTTTGAAGAACAAAACAATCCTCAAATACTC CAAAACTCAGTTGTTTTTGGAACATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGA ATTGAGCAGAAAACAACACTGACAGCTAGTGCAGGCATTGCCCCAAATACAATGTTAGCA AAAGTATGCAGTGATAAGAATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAA GCTGTGATGGACTTCATCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTT ACAGAGAAAATGTTAAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGG GCATTGCTTTCTCTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTG GGTCTAGGTTCAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAGG ACATTCAGTGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGCAGT GAGCTTGCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTAAGTTG AAGAATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGTTTCTACT GCAGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGATGCTGATTTT CCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTCCCAATGAAGAG GACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGGAAACCAAGCCCTG TCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTTGTAAAACCTCTAGAA ATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAAGGAAATGGAGTCACCAA GATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCAGACATCACAACCATTCCAA GTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCAGAGAATTCAGATGACTGTCAG ATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGTGCATCAGTCTGGAAGCCTTGAAT AAACATGTAGATGAATGTCTTGATGGACCTTCAATCAGTGAAAACTTTAAAATGTTCTCG TGTTCACATGTTTCTGCTACCAAAGTTAACAAGAAAGAAAATGTTCCTGCTTCTTCACTT TGTGAGAAGCAAGATTATGAAGCCCATCCAAAAATTAAAGAAATATCTTCAGTAGATTGT ATAGCTTTAGTAGATACTATAGATAACTCATCTAAAGCAGAAAGCATAGATGCTTTAAGT AATAAGCATAGCAAGGAAGAATGTTCTAGTCTCCCAAGCAAGTCTTTTAATATTGAACAC TGTCATCAGAATTCTTCTTCTACTGTTTCATTGGAAAACGAAGATGTTGGATCATTTAGA CAAGAATACCGCCAGCCTTACTTATGTGAAGTGAAAACAGGCCAAGCTCTAGTTTGTCCT GTTTGTAACGTAGAACAAAAGACTTCAGATCTAACCCTGTTCAATGTGCATGTGGATGTT TGCTTAAATAAAAGTTTTATCCAAGAATTAAGAAAGGATAAATTTAACCCAGTTAATCAA CCCAAAGAAAGCTCCAGAAGTACTGGTAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGA ACAAAAAGGCCAGGATTGATGACAAAGTACTCAACATCAAAGAAAATAAAACCAAACAAT CCCAAACATACCCTTGATATATTTTTTAAGTAA Clone variation with respect to NM_016218.2 927 c=>t 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_016218 unedited TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAACGGATAAGTTTATACC ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCTTAGGATGGGA CTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATTAACAAAATTATA ATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGAAAGAAAAGCAAGTC AACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCACCAGCCAACAGCTAAGA AAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAACAAAGCCGAAATTTGAGC AATACCATAGTGCACATTGACATGGATGCTTTCTATGCAGCTGTAGAAATGAGGGACAAT CCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAATGAGTATGCTGTCTACTTCAAAT TACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATGCCAGGATTTATTGCTAAGAGGCTG TGCCCACAACTTATAATAGTGCCCCCCAACTTTGACAAATACCGAGCTGTGAGTAAAGAG GTTAAGGAAATACTTGCTGATTATGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCC TACTTGAATATAACAAAGCACTTAGAAGAAAGAACAAATTGGCCTGAGGATAAAAGAAGG TATTTCATCAAAATGGGAAGCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTAATANAC TGAGTGAGCATGAACGATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGC AGCCCCCAGNAGATCCTTTTCCAGTGACTTTGAAGAACAAACATNCTCAATACTCAAACT CAGTGTTTTTGACATAGCCAGGAGTGGTAAGGAAATCGTTTAGAATGACAAAACACCTGA CGCTGTGCAGCATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_016218 unedited GGCCGCATTTCTANATCGAGTTTTTTTTTTTTTTTTTTATCGTTTTAGTAGAGACAGGGT CTCACCATGTTGGCGAGGCTGGTCTCAAACTCCTGACCTCAAGTGATCTGCCCATTCGGC CTCCCAAAGGGCTGGCATTACAGGTGTGAGCCATTGTGCCTGGCCTAACTAATTATTTTT TGAGAAGAACTCATTGATAGCACAAAAGTACTTGGAGAAACTACTTAAGCTAACTACTAT TAAGCTGTCTTCTTTCACAAATATTAATAAAAGTACTGATAGAAATGTAACTAAGATACA TAATGTGTTTTAATACACATTAATCTTTTGAATTACACATTTTCTCTTGAGGATTATAAT AAAATGATGGTACAATTTATCCTTCTTTATAGATTTGTGGAAAGTACCAACATAATTGAC AAGAAATGAAATACTGCCATTTAAAAGTCCTGTATTAAAATAAAACTACTTTGAATCATC TTAGACTAAATATTTTAAAAACTGATTCAAGTGCTTATTCATAAGATAATGGAATTTTAA ATAAGAAAAGCCAAATTAAAAATTATATTTTAGGCAAAAACCATTTTAACACAGATAATA CTGTGATATTTATTTTCCTGAAGAAAACCACTTATGCTAGTTACATTAATACTCTTCATA GAACTTAAGATAAATTCTATTATGAATGCTCATGAAGTATCTAAAGACACTAGGATCAAT GCCAACANAGTAGTTAAGGGCTGAAAATTCTTATGATATATAGGCATGTGAGAATCTTTG AGAGCCCTACAAAGTTTTTATTTCAGTTAAAATTATAAAAAATCACTTTCTTTCCCATTT CTAATTCCCCTTTAAGAATTTATCCTTAACAGATTGATTTCCATTGATGTTTATAGAAGG GAAATTCTCATGGCTATAAAAGAACCCCGAATCCGTTTGTTTCTTAAAAAGCACCTCTCT GGGAAGGTT
Restriction Sites	NotI-NotI
ACCN	NM_016218
Insert Size	3900 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016218.1, NP_057302.1
RefSeq Size	4074 bp
RefSeq ORF	2613 bp
Locus ID	51426
UniProt ID	Q9UBT6
Domains	IMS, ZnF_Rad18
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC219806	POLK (Myc-DDK-tagged)-Human polymerase (DNA directed) kappa (POLK)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,376.00 CNY 7,130.00
RC219806L1	Lenti ORF clone of Human polymerase (DNA directed) kappa (POLK), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,776.00 CNY 9,030.00
RC219806L2	Lenti ORF clone of Human polymerase (DNA directed) kappa (POLK), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
RC219806L3	Lenti ORF clone of Human polymerase (DNA directed) kappa (POLK), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
RC219806L4	Lenti ORF clone of Human polymerase (DNA directed) kappa (POLK), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,030.00
RG219806	POLK (tGFP-tagged) - Human polymerase (DNA directed) kappa (POLK)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,890.00

其它POLK产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies