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PARP9 (NM_001146105) Human Untagged Clone
CAT＃: SC327655
PARP9 (untagged)-Human poly (ADP-ribose) polymerase family member 9 (PARP9) transcript variant 5
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARTD9; BAL; BAL1; MGC:7868 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001146105, the custom clone sequence may differ by one or more nucleotides
ATGGACTTTTCCATGGTGGCCGGAGCAGCAGCTTACAATGAAAAATCAGAGACTGGTGCT CTTGGAGAAAACTATAGTTGGCAAATTCCCATTAACCACAATGACTTCAAAATTTTAAAA AATAATGAGCGTCAGCTGTGTGAAGTCCTCCAGAATAAGTTTGGCTGTATCTCTACCCTG GTCTCTCCAGTTCAGGAAGGCAACAGCAAATCTCTGCAAGTGTTCAGAAAAATGCTGACT CCTAGGATAGAGTTATCAGTCTGGAAAGATGACCTCACCACACATGCTGTTGATGCTGTG GTGAATGCAGCCAATGAAGATCTTCTGCATGGGGGAGGCCTGGCCCTGGCCCTGGTAAAA GCTGGTGGATTTGAAATCCAAGAAGAGAGCAAACAGTTTGTTGCCAGATATGGTAAAGTG TCAGCTGGTGAGATAGCTGTCACGGGAGCAGGGAGGCTTCCCTGCAAACAGATCATCCAT GCTGTTGGGCCTCGGTGGATGGAATGGGATAAACAGGGATGTACTGGAAAGCTGCAGAGG GCCATTGTAAGTATTCTGAATTATGTCATCTATAAAAATACTCACATTAAGACAGTAGCA ATTCCAGCCTTGAGCTCTGGGATTTTTCAGTTCCCTCTGAATTTGTGTACAAAGACTATT GTAGAGACTATCCGGGTTAGTTTGCAAGGGAAGCCAATGATGAGTAATTTGAAAGAAATT CACCTGGTGAGCAATGAGGACCCTACTGTTGCTGCCTTTAAAGCTGCTTCAGAATTCATC CTAGGGAAGAGTGAGCTGGGACAAGAAACCACCCCTTCTTTCAATGCAATGGTCGTGAAC AACCTGACCCTCCAGATTGTCCAGGGCCACATTGAATGGCAGACGGCAGATGTAATTGTT AATTCTGTAAACCCACATGATATTACAGTTGGACCTGTGGCAAAGTCAATTCTACAACAA GCAGGAGTTGAAATGAAATCGGAATTTCTTGCCACAAAGGCTAAACAGTTTCAACGGTCC CAGTTGGTACTGGTCACAAAAGGATTTAACTTGTTCTGTAAATATATATACCATGTACTG TGGCATTCAGAATTTCCTAAACCTCAGATATTAAAACATGCAATGAAGGAGTGTTTGGAA AAATGCATTGAGCAAAATATAACTTCCATTTCCTTTCCTGCCCTTGGGACTGGAAACATG GAAATAAAGAAGGAAACAGCAGCAGAGATTTTGTTTGATGAAGTTTTAACATTTGCCAAA GACCATGTAAAACACCAGTTAACTGTAAAATTTGTGATCTTTCCAACAGATTTGGAGATA TATAAGGCTTTCAGTTCTGAAATGGCAAAGAGGTCCAAGATGCTGAGTTTGAACAATTAC AGTGTCCCCCAGTCAACCAGAGAGGAGAAAAGAGAAAATGGGCTTGAAGCTAGATCTCCT GCCATCAATCTGATGGGATTCAACGTGGAAGAGATGTATGAGGCCCACGCATGGATCCAA AGAATCCTGAGTCTCCAGAACCACCACATCATTGAGAATAATCATATTCTGTACCTTGGG AGAAAGGAACATGACATTTTGTCTCAGCTTCAGAAAACTTCAAGTGTCTCCATCACAGAA ATTATCAGCCCAGGAAGGACAGAGTTAGAGATTGAAGGAGCCCGGGCTGACCTCATTGAG GTGGTTATGAACATTGAAGATATGCTTTGTAAAGTACAGGAGGAAATGGCAAGGAAAAAG GAGCGAGGCCTTTGGCGCTCGTTAGGACAGTGGACTATTCAGCAACAAAAAACCCAAGAC GAAATGAAAGAAAATATCATATTTCTGAAATGTCCTGTGCCTCCAACTCAAGAGCTTCTA GATCAAAAGAAACAGTTTGAAAAATGTGGTTTGCAGGTTCTAAAGGTGGAGAAGATAGAC AATGAGGTCCTTATGGCTGCCTTTCAAAGAAAGAAGAAAATGATGGAAGAAAAACTGCAC AGGCAACCTGTGAGCCATAGGCTGTTTCAGCAAGTCCCATACCAGTTCTGCAATGTGGTA TGCAGAGTTGGCTTTCAAAGAATGTACTCGACACCTTGCGATCCAAAATACGGAGCTGGC ATATACTTCACCAAGAACCTCAAAAACCTGGCAGAGAAGGCCAAGAAAATCTCTGCTGCA GATAAGCTGATCTATGTGTTTGAGGCTGAAGTACTCACAGGCTTCTTCTGCCAGGGACAT CCGTTAAATATTGTTCCCCCACCACTGAGTCCTGGAGCTATAGATGGTCATGACAGTGTG GTTGACAATGTCTCCAGCCCTGAAACCTTTGTTATTTTTAGTGGCATGCAGGCTATACCT CAGTATTTGTGGACATGCACCCAGGAATATGTACAGTCACAAGATTACTCATCAGGACCA ATGAGACCCTTTGCACAGCATCCTTGGAGGGGATTCGCAAGTGGCAGCCCTGTTGAT |
| Restriction Sites | Please inquire |
| ACCN | NM_001146105 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146105.1, NP_001139577.1 |
| RefSeq Size | 3072 bp |
| RefSeq ORF | 2460 bp |
| Locus ID | 83666 |
| UniProt ID | Q8IXQ6 |
| Gene Summary | ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses (PubMed:16809771, PubMed:23230272, PubMed:26479788, PubMed:27796300). Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose) (PubMed:28525742). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones (PubMed:28525742). During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1 (PubMed:23230272). Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272, PubMed:28525742). In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ); the complex function is negatively modulated by PARP9 activity (PubMed:28525742). Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR) (By similarity). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation (PubMed:27796300). Also suppresses PARP14-mediated STAT6 ADP-ribosylation (PubMed:27796300).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) differs in the 5' UTR and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a. Variants 3, 4 and 5 encode the same isoform (b). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229020 | PARP9 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 5 |
CNY 8,170.00 |
|
| RC229020L3 | Lenti-ORF clone of PARP9 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 5 |
CNY 10,070.00 |
|
| RC229020L4 | Lenti-ORF clone of PARP9 (mGFP-tagged)-Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 5 |
CNY 10,070.00 |
|
| RG229020 | PARP9 (tGFP-tagged) - Human poly (ADP-ribose) polymerase family, member 9 (PARP9), transcript variant 5 |
CNY 9,030.00 |
