MAK (NM_001242957) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RP62
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331937 representing NM_001242957. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAACCGATACACAACCATGAGACAGTTGGGGGACGGCACGTATGGGAGTGTGCTTATGGGCAAGAGT AATGAATCCGGGGAGCTGGTGGCCATCAAAAGGATGAAGAGAAAGTTCTATTCTTGGGATGAATGCATG AACTTGAGAGAAGTTAAGTCTCTGAAGAAACTTAATCATGCCAATGTTATTAAATTGAAAGAAGTTATC AGAGAAAATGACCATCTTTATTTTATATTTGAATATATGAAAGAAAACCTCTATCAATTAATGAAAGAC AGAAACAAGTTGTTCCCTGAATCAGTCATCAGAAATATTATGTATCAAATATTGCAAGGGCTGGCTTTT ATCCATAAACATGGCTTTTTTCATAGGGACATGAAACCAGAAAACTTGCTTTGTATGGGTCCAGAGCTT GTGAAAATTGCTGATTTTGGACTTGCAAGAGAATTAAGGTCACAGCCACCATACACTGATTATGTATCT ACCAGATGGTATCGTGCCCCTGAAGTTTTACTGAGATCTTCAGTTTATAGTTCTCCCATTGATGTGTGG GCTGTTGGAAGTATCATGGCTGAACTCTATATGTTAAGGCCACTTTTCCCAGGGACAAGTGAGGTCGAT GAAATCTTTAAAATTTGCCAAGTTTTAGGGACTCCCAAAAAAAGTGACTGGCCAGAAGGATACCAGCTG GCATCCTCTATGAACTTCCGTTTTCCCCAGTGTGTTCCTATAAACTTAAAAACTCTTATTCCCAATGCC AGTAATGAAGCTATTCAGCTCATGACCGAAATGTTGAATTGGGATCCAAAGAAACGACCGACAGCAAGC CAGGCATTGAAACACCCATATTTTCAAGTTGGTCAGGTATTAGGCCCTTCGTCAAATCATCTGGAATCA AAACAGTCTTTAAATAAGCAGCTGCAACCATTAGAATCAAAGCCATCTTTAGTTGAGGTAGAGCCTAAG CCTCTGCCGGATATAATCGATCAGGTTGTTGGACAACCCCAGCCAAAAACTAGCCAGCAGCCACTGCAG CCCATTCAGCCGCCACAGAACCTGAGCGTCCAGCAACCTCCAAAGCAACAGAGTCAGGAGAAACCGCCA CAAACGCTATTCCCGAGCATCGTCAAAAACATGCCAACTAAGCCAAATGGCACACTGAGTCATAAAAGT GGTAGGAGGCGTTGGGGTCAGACTATCTTCAAGTCTGGAGATAGCTGGGAAGAGTTGGAGGACTATGAT TTCGGAGCCTCCCATTCCAAGAAGCCAAGCATGGGTGTTTTTAAAGAAAAAAGGAAAAAAGATTCTCCA TTTCGGCTTCCAGAGCCAGTACCCTCAGGCTCCAACCACTCGACAGGGGAAAACAAGAGCTTACCTGCT GTTACTTCCCTAAAATCTGATTCCGAATTGTCAACTGCTCCAACCTCTAAACAGTACTACTTGAAACAA TCAAGATATCTTCCAGGTGTGAATCCCAAGAAGGTGTCCTTGATAGCCAGTGGAAAGGAAATAAACCCC CACACTTGGAGCAACCAGTTATTCCCCAAGTCACTGGGACCCGTTGGGGCAGAACTTGCTTTCAAAAGG AGCAATGCAGAAGAAAGCATAATTAAACCAATCGAAAAACTATCATGCAATGAAACTTTTCCTGAAAAA TTAGAGGACCCACAAGGAAATCTTGGAAGTTATGCTACTTACAATCAGTCAGGATATATTCCTTCCTTT CTCAAAAAAGAAGTGCAGTCAGCTGGCCAGAGGATCCACTTAGCACCTCTCAATGCAACGGCTTCAGAA TATACCTGGAACACAAAAACTGGTCGGGGGCAGTTTTCAGGACGTACTTATAATCCTACAGCAAAAAAC CTAAATATTGTGAACCGTGCACAGCCCATTCCCTCAGTGCATGGGAGGACAGACTGGGTGGCCAAGTAT GGAGGCCACCGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001242957
Insert Size	1947 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242957.1
RefSeq Size	3974 bp
RefSeq ORF	1947 bp
Locus ID	4117
UniProt ID	P20794
Protein Families	Druggable Genome, Protein Kinase
MW	73.5 kDa
Gene Summary	The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (3) contains an alternate in-frame exon compared to variant 1. The encoded isoform (3) is longer than isoform 1.

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