Itgax Hamster Monoclonal Antibody [Clone ID: N418]

Specifications

Product Data
Clone Name	N418
Applications	FC
Recommend Dilution	Flow Cytometry: 1/5 - 1/10; use 10µl of the suggested working dilution to label 10e6 cells in 100µl; The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Mouse spleen dendritic cells.
Specificity	This antibody recognises the murine homologue of human CD11c, which is a 150/90kD member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells. Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Formulation	Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide, 1% Bovine Serum Albumin Label: Biotin State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin alpha X
Database Link	Entrez Gene 16411 Mouse UniProt ID Q9QXH4
Background	CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms	ITGAX, Integrin alpha-X, Leu M5
Reference Data

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