PKC theta (PRKCQ) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 1C2]

Specifications

Product Data
Clone Name	1C2
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated MCF-7 cells (See Protocols for more details).
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin derived from the C-terminal domain of PKC theta.
Specificity	This monoclonal antibody 1C2 recognizes Protein kinase C theta (PKC theta).
Formulation	1 ml PBS with 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	protein kinase C theta
Database Link	Entrez Gene 85420 RatEntrez Gene 5588 Human UniProt ID Q04759
Background	Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. The protein kinase C family of Ser/Thr kinases consists of 11 isoenzymes. PKC theta is a autophosphorylated protein kinase, a member of the novel PKC (nPKC) subfamily. PKC theta is expressed in most tissues.
Synonyms	PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type
Note	Mol. weight: 79 kD Protocol: Positive Control Provided. Cell lysate from untreated MCF-7 cells Description: Cell lysate from untreated MCF-7 cells, breast adenocarcinoma (Human) Format: Lyophilized cell lysate from serum starved MCF-7 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	Adipocytokine signaling pathway, T cell receptor signaling pathway, Tight junction, Vascular smooth muscle contraction

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