Map1lc3b (NM_026160) Mouse Tagged ORF Clone
CAT#: MG200654
- TrueORF®
Map1lc3b (tGFP-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b)
ORF Plasmid: DDK
"NM_026160" in other vectors (4)
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CNY 2,800.00
CNY 2,850.00
Cited in 2 publications. |
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 1010001C15Rik; Atg8; LC3b; Map1lc3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200654 representing NM_026160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCCGAGAAGACCTTCAAGCAGCGCCGGAGCTTTGAACAAAGAGTGGAAGATGTCCGGCTCATCC GGGAGCAGCACCCCACCAAGATCCCAGTGATTATAGAGCGATACAAGGGGGAGAAGCAGCTGCCCGTCCT GGACAAGACCAAGTTCCTGGTGCCTGACCACGTGAACATGAGCGAGCTCATCAAGATAATCAGACGGCGC TTGCAGCTCAATGCTAACCAAGCCTTCTTCCTCCTGGTGAATGGGCACAGCATGGTGAGTGTGTCCACTC CCATCTCCGAAGTGTACGAGAGTGAGAGAGATGAAGACGGCTTCCTGTACATGGTTTATGCCTCGCAGGA GACATTCGGGACAGCAATGGCTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200654 representing NM_026160
Red=Cloning site Green=Tags(s) MPSEKTFKQRRSFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRR LQLNANQAFFLLVNGHSMVSVSTPISEVYESERDEDGFLYMVYASQETFGTAMAV TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_026160 |
ORF Size | 375 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026160.5 |
RefSeq Size | 1711 bp |
RefSeq ORF | 378 bp |
Locus ID | 67443 |
UniProt ID | Q9CQV6 |
Gene Summary | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Autophagy induction promotes renal cyst growth in polycystic kidney disease
,Lee, EJ;Ko, JY;Oh, S;Jun, J;Mun, H;Lim, CJ;Seo, S;Ko, HW;Kim, H;Oh, YK;Ahn, C;Kang, M;Kim, MJ;Yoo, KH;Oh, GT;Park, JH;,
EBioMedicine
,PubMed ID 32949996
[MAP1LC3B]
|
Cross-talk between miR-471-5p and autophagy component proteins regulates LC3-associated phagocytosis (LAP) of apoptotic germ cells
,null,
Nature Communications
,PubMed ID 28928467
[Map1lc3b]
|
Documents
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FAQs |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203443 | Map1lc3b (untagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b), (10ug) |
CNY 1,200.00 |
|
MR200654 | Map1lc3b (Myc-DDK-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) |
CNY 1,200.00 |
|
MR200654L3 | Lenti ORF clone of Map1lc3b (Myc-DDK-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) |
CNY 4,750.00 |
|
MR200654L4 | Lenti ORF clone of Map1lc3b (mGFP-tagged) - Mouse microtubule-associated protein 1 light chain 3 beta (Map1lc3b) |
CNY 3,600.00 |
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