Ufd1 (NM_011672) Mouse Tagged ORF Clone

CAT#: MG204334

  • TrueORF®

Ufd1l (tGFP-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l)

ORF Plasmid: DDK tGFP



  "NM_011672" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Ufd1; Ufd1l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG204334 representing NM_011672
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTCTTTCAACATGTTTGACCACCCGATTCCCCGGGTCTTCCAGAACCGCTTCTCCACGCAGTACC
GCTGCTTCTCCGTGTCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGATAAT
TATGCCACCCTCAGCCCTCGATCAACTCAGCCGGCTCAACATTACCTATCCTATGCTGTTTAAATTGACC
AATAAGAATTCAGATCGGATGACACACTGCGGTGTACTGGAGTTTGTTGCTGATGAAGGCATCTGTTACC
TCCCCCACTGGATGATGCAGAATTTGCTGTTGGAGGAAGGGGGCCTGGTTCAGGTGGAAAGTGTCAACCT
CCAAGTGGCGACCTACTCTAAGTTCCAGCCTCAGAGCCCAGACTTCCTGGATATTACCAACCCTAAAGCG
GTATTAGAAAATGCATTGAGAAACTTCGCCTGTCTGACGACTGGAGATGTGATTGCTATCAACTACAATG
AGAAGATCTATGAGCTGCGGGTGATGGAGACCAAACCTGACAAGGCTGTATCCATTATTGAATGTGACAT
GAATGTGGATTTTGATGCTCCCTTGGGATACAAAGAACCAGAAAGACCAGTGCAGCATGAGGAGTCAATA
GAGGGAGAAGCTGACCACAGTGGCTATGCCGGAGAGGTGGGCTTCCGTGCCTTCTCTGGTTCTGGGAATA
GACTGGATGGGAAGAAAAAAGGGGTTGAGCCCAGTCCCTCCCCAATCAAGCCTGGAGACATCAAAAGAGG
AATTCCTAATTACGAATTTAAGCTTGGTAAGATCACTTTCATCAGAAATTCACGTCCATTGGTCAAAAAG
GTTGAAGAGGATGAAGCTGGAGGCAGATTCATTGCTTTCTCTGGAGAAGGACAGTCACTGCGTAAGAAGG
GAAGAAAGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG204334 representing NM_011672
Red=Cloning site Green=Tags(s)

MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLT
NKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKA
VLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERPVQHEESI
EGEADHSGYAGEVGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKK
VEEDEAGGRFIAFSGEGQSLRKKGRKP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011672
ORF Size 921 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011672.4, NP_035802.3
RefSeq Size 1959 bp
RefSeq ORF 924 bp
Locus ID 22230
UniProt ID P70362
Gene Summary Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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