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Grb7 (NM_010346) Mouse Tagged ORF Clone
CAT＃: MG208570
- TrueORF®
Grb7 (tGFP-tagged) - Mouse growth factor receptor bound protein 7 (Grb7)
ORF Plasmid: DDK
"NM_010346" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA4028 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208570 representing NM_010346
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTGGATCTGAGCCCGACTCATCTCAGCAGCTCCCCAGAAGATGTGTGCCCAACTCCTGCTACCC CTCCTGAGACTCCTCCGCCCCCTGATAACCCTCCGCCAGGGGATGTGAAGCGGTCGCAGCCTTTGCCCAT CCCCAGCAGCAGGAAACTTCGAGAAGAGGAGTTTCAGGCAACCTCTCTGCCTTCCATCCCCAACCCCTTC CCTGAGCTCTGCAGCCCACCTTCACAGAAACCCATTCTTGGTGGTTCCTCCGGTGCAAGGGGGTTGCTTC CTCGAGACTCCAGCCGCCTCTGTGTGGTGAAGGTGTACAGTGAGGATGGGGCCTGCCGGTCTGTGGAGGT GGCAGCGGGCGCCACAGCTCGTCACGTGTGTGAGATGCTGGTACAACGAGCTCACGCCCTGAGCGACGAG AGCTGGGGACTAGTGGAATCCCACCCCTACCTGGCACTGGAGCGGGGTCTGGAGGACCATGAATTTGTGG TGGAAGTGCAGGAGGCCTGGCCTGTGGGTGGAGACAGCCGCTTCATCTTTCGTAAAAACTTCGCCAAGTA TGAACTATTCAAGAGCCCCCCACACACCCTGTTCCCAGAAAAGATGGTCTCGAGCTGTCTGGATGCACAA ACAGGCATATCCCATGAAGACCTCATCCAGAACTTCCTGAACGCTGGCAGCTTCCCTGAGATCCAGGGCT TCCTGCAGCTGCGGGGATCAGGCCGGGGGTCAGGTCGAAAGCTTTGGAAACGTTTCTTCTGCTTTCTGCG TCGATCTGGCCTCTACTACTCTACCAAGGGTACCTCCAAGGACCCCAGACACCTACAGTATGTGGCAGAT GTGAATGAGTCCAATGTCTATGTGGTGACCCAGGGCCGCAAGCTGTATGGGATGCCCACTGACTTCGGCT TCTGTGTCAAGCCCAACAAGCTTCGAAACGGCCACAAGGGGCTCCACATCTTCTGCAGTGAGGATGAGCA GAGTCGGACCTGCTGGCTGGCTGCCTTCCGGCTCTTCAAGTACGGGGTACAGCTATATAAGAATTATCAG CAGGCCCAGTCTCGTCACCTGCGCCTATCCTATTTGGGGTCTCCACCCTTGAGGAGCGTCTCAGACAATA CCCTAGTGGCTATGGACTTCTCTGGCCATGCGGGGCGTGTCATTGATAACCCCCGGGAAGCTCTGAGTGC CGCCATGGAGGAGGCCCAGGCCTGGAGGAAGAAGACAAACCACCGTCTGAGCCTGCCCACCACATGCTCT GGCTCGAGCCTCAGCGCAGCCATTCATCGCACCCAGCCCTGGTTTCATGGACGCATCTCTCGGGAGGAGA GCCAGCGGCTAATTGGACAGCAGGGCCTGGTGGATGGTGTGTTCCTGGTCCGGGAGAGCCAGAGGAACCC ACAGGGCTTTGTCCTGTCCTTGTGCCATCTGCAGAAAGTCAAGCATTATCTCATTTTGCCAAGCGAAGAT GAAGGTTGCCTTTACTTCAGCATGGATGAGGGCCAGACCCGTTTCACAGACCTGCTGCAGCTGGTAGAAT TCCACCAGCTGAACCGAGGCATCCTGCCCTGCCTGCTGCGCCACTGCTGTGCCCGTGTGGCCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208570 representing NM_010346
Red=Cloning site Green=Tags(s) MELDLSPTHLSSSPEDVCPTPATPPETPPPPDNPPPGDVKRSQPLPIPSSRKLREEEFQATSLPSIPNPF PELCSPPSQKPILGGSSGARGLLPRDSSRLCVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDE SWGLVESHPYLALERGLEDHEFVVEVQEAWPVGGDSRFIFRKNFAKYELFKSPPHTLFPEKMVSSCLDAQ TGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVAD VNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLHIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQ QAQSRHLRLSYLGSPPLRSVSDNTLVAMDFSGHAGRVIDNPREALSAAMEEAQAWRKKTNHRLSLPTTCS GSSLSAAIHRTQPWFHGRISREESQRLIGQQGLVDGVFLVRESQRNPQGFVLSLCHLQKVKHYLILPSED EGCLYFSMDEGQTRFTDLLQLVEFHQLNRGILPCLLRHCCARVAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010346 |
| ORF Size | 1605 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010346.2, NP_034476.1 |
| RefSeq Size | 2413 bp |
| RefSeq ORF | 1608 bp |
| Locus ID | 14786 |
| UniProt ID | Q03160 |
| Gene Summary | Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration (By similarity). Plays a role in the assembly and stability of RNA stress granules. Binds to the 5'UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200123 | Grb7 (untagged) - Mouse growth factor receptor bound protein 7 (Grb7), (10ug) |
CNY 2,000.00 |
|
| MR208570 | Grb7 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 7 (Grb7) |
CNY 3,992.00 |
|
| MR208570L3 | Lenti ORF clone of Grb7 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 7 (Grb7) |
CNY 6,460.00 |
|
| MR208570L4 | Lenti ORF clone of Grb7 (mGFP-tagged) - Mouse growth factor receptor bound protein 7 (Grb7) |
CNY 6,460.00 |
