Dnm1l (BC079635) Mouse Tagged ORF Clone

CAT#: MG210122

  • TrueORF®

Dnm1l (tGFP-tagged) - Mouse dynamin 1-like (cDNA clone MGC:90593 IMAGE:6400313)



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Mouse Protein / Lysate Request

CNY 6,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DNM1L Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 6330417M19Rik; AI450666; Dlp1; Dnmlp1; Drp1; python
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210122 representing BC079635
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCTGATCCCGGTCATCAATAAGCTGCAGGACGTCTTCAACACAGTGGGTGCGGACATCATCC
AGCTGCCTCAGATCGTCGTAGTGGGAACGCAGAGCAGTGGGAAGAGCTCAGTGCTGGAAAGCCTAGTGGG
CAGGGACCTTCTTCCCAGAGGAACTGGTGTGGTCACCCGGAGACCTCTCATTCTGCAGCTAGTCCACGTT
TCACCAGAAGATAAAAGAAAAACAACAGGAGAAGAAAATGGAGTTGAAGCAGAAGAATGGGGTAAATTTC
TTCACACCAAAAACAAGCTTTACACAGATTTTGATGAAATTCGACAAGAAATTGAAAATGAAACTGAAAG
AATTTCAGGAAATAATAAGGGGGTAAGCCCTGAGCCAATCCATCTCAAGGTTTTCTCGCCCAACGTTGTC
AACCTGACACTTGTGGATTTACCGGGAATGACCAAAGTACCTGTAGGCGATCAGCCCAAGGACATCGAGC
TTCAGATCAGAGAACTTATTCTTCGGTTCATCAGTAATCCCAATTCCATTATCCTCGCCGTCACTGCTGC
AAATACAGATATGGCAACATCAGAAGCACTCAAGATTTCCAGAGAGGTAGATCCAGATGGGCGCAGAACT
CTAGCTGTAATCACTAAACTTGATCTCATGGATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGG
TTATTCCAGTCAAGCTTGGAATAATTGGAGTAGTTAACAGAAGCCAACTGGATATTAACAATAAGAAGAG
TGTAACTGATTCAATCCGTGATGAGTATGCTTTTCTTCAAAAGAAGTACCCATCTCTGGCCAACAGAAAT
GGAACAAAGTATCTTGCTAGGACCCTGAATAGGTTACTTATGCATCATATCAGAGATTGTTTACCAGAGC
TGAAAACAAGAATAAATGTCTTAGCTGCTCAGTATCAGTCTCTTCTAAATAGCTATGGTGAACCGGTGGA
TGATAAAAGTGCTACTTTACTCCAGCTTATTACCAAATTTGCCACAGAGTATTGTAACACGATTGAAGGA
ACCGCAAAGTACATTGAAACTTCTGAGCTATGCGGTGGTGCTAGGATTTGTTATATTTTCCATGAGACTT
TCGGGCGAACCTTAGAATCTGTGGACCCACTAGGTGGCCTTAACACTATTGACATCCTGACTGCCATCAG
AAATGCTACTGGCCCCCGTCCTGCTTTATTTGTGCCTGAAGTTTCATTTGAGTTACTGGTCAAACGTCAG
ATTAAGCGTCTAGAAGAGCCCAGCCTACGGTGTGTGGAGCTGGTCCATGAGGAGATGCAGAGGATCATTC
AGCATTGTAGCAATTACAGCACACAGGAATTGTTACGGTTCCCTAAACTTCACGATGCCATAGTTGAAGT
AGTGACCTGTCTTCTTCGTAAAAGGTTGCCCGTGACAAATGAAATGGTGCATAACTTAGTGGCAATTGAG
CTAGCGTATATCAACACAAAACACCCCGACTTTGCTGATGCCTGTGGGCTAATGAACAATAATATAGAGG
AACAAAGAAGAAACAGGCTAGCAAGAGAGCTGCCTTCAGCTGGATCACGGGACAAGGTCCCATCTGCAGG
TGGTGGGATTGGAGACGGTGGTCAGGAACCAACAACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAA
GCTGAAGAATTACTTGCTGAAGAAAAATCAAAACCAATTCCAATTATGCCAGCAAGTCCACAGAAAGGCC
ATGCTGTCAATTTGCTAGATGTGCCAGTTCCAGTTGCAAGAAAACTGTCTGCCCGAGAACAGCGAGATTG
TGAGGTTATTGAAAGACTTATCAAATCATATTTTCTAATTGTCAGAAAGAATATTCAAGACAGTGTCCCA
AAGGCAGTAATGCATTTTTTGGTTAATCATGTGAAAGATACTCTTCAGAGTGAACTGGTAGGGCAGCTGT
ATAAGTCATCCTTATTAGATGACCTTCTGACTGAATCCGAGGACATGGCCCAACGAAGAAAAGAAGCAGC
GGATATGCTGAAGGCATTACAAGGAGCCAGTCAAATTATTGCTGAAATCCGAGAGACTCATCTTTGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210122 representing BC079635
Red=Cloning site Green=Tags(s)

MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHV
SPEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVV
NLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRT
LAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRN
GTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEG
TAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQ
IKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIE
LAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAGSRDKVPSAGGGIGDGGQEPTTGNWRGMLKTSK
AEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVP
KAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC079635
ORF Size 2099 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079635, AAH79635
RefSeq Size 3872 bp
RefSeq ORF 2099 bp
Locus ID 74006
Gene Summary This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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