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Bbs7 (NM_027810) Mouse Tagged ORF Clone
CAT＃: MG210222
- TrueORF®
Bbs7 (tGFP-tagged) - Mouse Bardet-Biedl syndrome 7 (Bbs7)
ORF Plasmid: DDK
"NM_027810" in other vectors (4)
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CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 8430406N16Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210222 representing NM_027810
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGACGTTAAGCCGGGCAGACTACCTCCAGGTCGGAGTGACCTCTCAGAAAACTATGAAGTTGC TTCCTACCTCAAGACAAAGGGCTACACAAAAGGTGGTTGTTGGAGATCAAGATGGGGTAGTGATATGCTT CGGCGTGAAGAAAGGAGAAGCTGTGCCAGTGTTCAAGACTTTACCAGGGCAGAAGATCTCGAGGCTGGAA CTAGGAGGGGCCGTCAACACGCCTCAGGAGAAAATCTTCATTGCTGCAGGGTCCGAGATTAGAGGCTTTA CAAAGAGAGGGAAACAGTTTCTCTCTTTTGAAACTAACCTCACGGAAAGCATTAAAGCTATGTACATATC TGGCTCTGACCTCTTCCTCAGTGCAAGCTACATCTATAATCACTACTGTGATTGCAAAGACCAAAATTAT TACCTTTCTGGGGACAAAATCAATGATGTCATCTGCCTTCCTGTGGAAAAGTTATCTCGCGTGACACCGG TGTTGGCCTGCCAGGACCGGGTGCTCCGAGTCTTACAGGGATCTGATGTGATGTATGAAATTGAGGTTCC TGGTCCACCCACCGTCTTAGCACTGCATAATGGAGATGGAGGTGACTCTGGAGAAGGACTTCTGTTCGGC ACATCAGATGGAAGACTTGGGCTTATTCAGATCACAACATCCAAACCAATACACAAATGGGAAATTCGAA ATGACAAAAAGCGGGGAGGTATCTTGTGTGTTGACAGCTTTGATATCATGGGTGATGGGGTTAAAGATTT ACTTGTTGGGAGAGATGATGGAATGGTGGAAGTATATAGTTTTGAAAATGCAAATGAACCTGTGCTACGA TTTGATCAGATGCTGTCTGAAAGCGTCACCTCCATCCAGGGTGGCTGTGTGGGAAAAGATGGCTATGATG AAATCGTGCTGGCCACATATTCAGGCTGGGTGACAGGGCTGACTACAGAGCCAACTCATAAAGAGAGTGG ACCAGGAGAAGAACTAAAACTTAATCAGGAGATGCAGAATAAGATTTCTTCTTTAAGGTCTGAAATAGAA CACCTGCAGTTTAAGGTGCTTCAGGAGAGAGAAAATTACCAACAGTCTTCACAGTCAAGCCAAGCAAAGT CAACAGTCCCTTCATTCAGTATAAATGACAAGTTCACGTTGAATAAAGAAGATGCTAGCTACAGCCTCGT CTTAGAGGTGCGGACTGCAATAGACAATGTCCTAATACAGAGTGATGTTCCAATAGATCTACTTGATGTG GACAAGAATTCTGCTGTTGTGAGCTTCAGCAGCTGTGACACAGAGTCAAACGACAACTTTCTTCTTGCTA CTTATCGGTGCCAGGCAAATACCACCAGGCTGGAGCTCAAGATTCGTTCAATTGAAGGCCAGTATGGCAC ACTCCAGGCATATGTGACTCCAAGAATCCAACCAAAAACCTGTCAGGTTCGCCAATACCACATCAAACCT CTATCACTCCATCAAAGAACTCACTTTATTGACCATGACAGGCCTATGAACACACTGACTCTAACAGGCC AGTTCAGCTTTGCTGAAGTTCACTCCTGGGTGGTGTTCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGC AGGGGAATGTGCAACATTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATACAGGAAA GGAGAAGGAGTTTTTAAATCTGACAACATTTCTACAATATCCATCCTAAAAGATGTGCTTTCTAAAGAAG CTACAAAAAGGAAAATTAATCTCAACATATCATACGAGATAAACGAGGTGTCTGTCAAGCACACTTTAAA GCTAATTCACCCGAAGCTGGAGTATCAGCTGCTTCTGGCTAAGAAAGTGCAGTTAATCGATGCCCTGAAG GAATTGCAGGTTCATGAGGGAAATACAGACTTTCTGACTCCAGAGTACCGATGTATTCTAGAAGAGGCAG ATCATCTACAAGAGGAATACAAAAAGCAGCCTGCACATCTTGAAAGACTCTATGGTATGATTACTGATCT TTTTATAGATAAATTTAAGTTCAAAGGCACTAATGTAAAAACCAAAGTACCCATGCTCCTGGAGATTCTC GACAGCTACGACCAGAACACACTGATTTCTTTCTTCGATGCAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210222 representing NM_027810
Red=Cloning site Green=Tags(s) MDLTLSRADYLQVGVTSQKTMKLLPTSRQRATQKVVVGDQDGVVICFGVKKGEAVPVFKTLPGQKISRLE LGGAVNTPQEKIFIAAGSEIRGFTKRGKQFLSFETNLTESIKAMYISGSDLFLSASYIYNHYCDCKDQNY YLSGDKINDVICLPVEKLSRVTPVLACQDRVLRVLQGSDVMYEIEVPGPPTVLALHNGDGGDSGEGLLFG TSDGRLGLIQITTSKPIHKWEIRNDKKRGGILCVDSFDIMGDGVKDLLVGRDDGMVEVYSFENANEPVLR FDQMLSESVTSIQGGCVGKDGYDEIVLATYSGWVTGLTTEPTHKESGPGEELKLNQEMQNKISSLRSEIE HLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDV DKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKP LSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRK GEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVKHTLKLIHPKLEYQLLLAKKVQLIDALK ELQVHEGNTDFLTPEYRCILEEADHLQEEYKKQPAHLERLYGMITDLFIDKFKFKGTNVKTKVPMLLEIL DSYDQNTLISFFDAA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027810 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027810.1, NP_082086.1 |
| RefSeq Size | 2594 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 71492 |
| UniProt ID | Q8K2G4 |
| Gene Summary | The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204070 | Bbs7 (untagged) - Mouse Bardet-Biedl syndrome 7 (human) (Bbs7), (10ug) |
CNY 2,000.00 |
|
| MR210222 | Bbs7 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 7 (human) (Bbs7) |
CNY 5,248.00 |
|
| MR210222L3 | Lenti ORF clone of Bbs7 (Myc-DDK-tagged) - Mouse Bardet-Biedl syndrome 7 (human) (Bbs7) |
CNY 8,170.00 |
|
| MR210222L4 | Lenti ORF clone of Bbs7 (mGFP-tagged) - Mouse Bardet-Biedl syndrome 7 (human) (Bbs7) |
CNY 8,170.00 |
