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Nolc1 (NM_053086) Mouse Tagged ORF Clone
CAT＃: MG215487
- TrueORF®
Nolc1 (tGFP-tagged) - Mouse nucleolar and coiled-body phosphoprotein 1 (Nolc1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_053086" in other vectors (2)
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CNY 7,700.00
货期*
4周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NOPP130; NOPP140; P130 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215487 representing NM_053086
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCTTTATCCCCTTGTGCTCAGATTTCTGCGGG ATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCGACCGGCGCTACACAGCAGGACGCCAATGC CTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAACAGGTCCACCAAAGCCCCAAAGGTGAAGTTACAG TCAAATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATCCAGTGACAGCAGTGAGGACAGCAGTG AGGACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCTGCCGCACAGGTCAAGCGAGCCAGTGT GCCTCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCAGCAGTAGTGAAGAATCCAGTGAGGAA GAGGAAGAGGACAAAAAGAAAAAGCCTGTCCAGAAGGCAGCTAAGCCCCAAGCCAAGGCAGTCAGACCTC CTGCGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGATTCGGACTCCAGCTCAGAGGAAGAAAC ACCACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTCAGACTAAAGCCGAAGCCAAACCAGGT ACACCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGCCGCCAGCAGCAGCAGCAGCAGCAGCA GCGATGACTCAGAGGAAGAGAAGAAGGCAGCTGCACCTCCCAAGAAGACTGTACCAAAAAAGCAAGTCGT GGCCAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCACCCAGAAGAGCTCCAGCAGTGAGGATTCTTCCAGT GAAGAGGAGGAGGGACAGAGACAACCCATGAAGAAAAAAGCAGGTCCCTACAGTTCAGTTCCACCACCCT CTGTTCCTTTACCAAAGAAGTCCCCGGGAACCCAGGCTCCAAAGAAAGCTGCTGCGCAGACACAGCCTGC AGACAGCAGTGACGACAGCAGTGACGATTCTGATTCAAGTTCTGAGGAAGAGAAAAAACCTCCAGCTAAG ACGGTCGTCTCCAAGACACCCGCCAAAGCAGCTCCAGTGAAGAAGAAAGCAGAAAGCTCTTCAGACAGCT CGGATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCCAAGCCAGC TGTCACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTAACCAGAAA CCTCAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGAGGAGGCCT CCAAGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAGGCCCCTCA GGCTGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTCCTAAGCCC CCAGCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGAGGCCAAGA GCAGCAGCAGCAGCAGCAGCAGCAGCTCCGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCCAAAGC TACTACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAGCCAAG GAAAGTGAGGAGGAAGAGGAGGAGGAAGAAACAGAAGAGAAGAAAAAGGCAGCTGGGACCAAGCCAGGTT CAGGCAAAAAACGGAAGCAGAATGAGACCGCAGATGAAGCAACAACTCCTCAAGCTAAGAAAGTTAAGCT CGAGACCCCCAATACGTTTCCAAAAAGGAAGAAGGGAGAAAGAAGGGCGTCTTCCCCTTTCCGAAGGGTC AGGGAGGAGGAGATTGAGGTGGACTCTCGAGTGGCGGACAATTCCTTTGATGCCAAGCGAGGTGCAGCTG GAGACTGGGGGGAGCGAGCCAATCAGGTTCTGAAGTTCACCAAAGGCAAGTCCTTCCGGCATGAAAAAAC GAAGAAGAAGCGAGGCAGCTACCGGGGAGGCTCCATCTCTGTCCAGGTCAATTCCGTCAAATTCGACAGC GAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215487 representing NM_053086
Red=Cloning site Green=Tags(s) MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLNRSTKAPKVKLQ SNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEE EEEDKKKKPVQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKPG TPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSSS EEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPAK TVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQK PQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPKP PAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAAK ESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRRV REEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFDS E TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053086 |
| ORF Size | 2103 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053086.3, NP_444316.2 |
| RefSeq Size | 3649 bp |
| RefSeq ORF | 2106 bp |
| Locus ID | 70769 |
| UniProt ID | E9Q5C9 |
| Gene Summary | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).[UniProtKB/Swiss-Prot Function] |
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