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Ubr5 (NM_001081359) Mouse Tagged ORF Clone
CAT＃: MG220944
- TrueORF®
Ubr5 (tGFP-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 5 (Ubr5) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001081359" in other vectors (2)
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CNY 33,730.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Edd; Edd1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220944 representing NM_001081359
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCATCCATTTCGTGGTCCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGGG AAGTTTCAGAGAAGCTGAACAAGTACAATTTGAACAGCCACCCACCTCTGAATGTACTGGAGCAGGCTAC TATTAAACAGTGTGTGGTGGGACCCAATCACGCCGCGTTTCTTCTTGAGGATGGCAGAATTTGTAGGATT GGTTTTTCAGTACAACCAGACAGACTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAACTGAACA GCAGCTCGGGGACAGGGAGGACCTCTAGGCCAGGAAGGACAAGTGACTCCCCGTGGTTTCTCTCAGGTTC TGAGACTCTGGGCAGGTTGGCAGGCAACACTCTGGGAAGCCGCTGGAGTTCTGGCGTGGGGGGAAGTGGA GGAGGCTCCTCTGGTCGGTCGTCTGCTGGAGCTCGTGATTCCCGGCGACAGACTCGAGTCATTCGGACTG GCCGGGATCGGGGGTCTGGACTCCTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCTGA GGAGCTGATATCACAGGCACAAGTTGTTCTACAAGGCAAATCGAGAAGTGTCATTATTCGAGAACTTCAG AGAACAAATCTTGATGTCAACCTTGCTGTAAATAACTTACTGAGCCGGGATGATGAGGATGGAGATGACG GTGATGACACAGCCAGTGAATCTTACCTGCCGGGAGAAGATCTTATGTCTCTTCTTGACGCTGACATTCA CTCTGCCCACCCAAGTGTCATTATTGATGCTGATGCCATGTTTTCTGAGGACATTAGCTATTTTGGTTAT CCTTCTTTTCGTCGTTCATCACTCTCTAGGCTAGGCTCATCACGAGTTCTCCTTCTTCCCTTAGAGAGAG ACTCAGAGCTTCTGCGGGAACGGGAGTCTGTTCTACGTTTACGGGAGCGGCGGTGGCTTGATGGAGCCTC ATTTGATAATGAAAGGGGCTCTACCAGCAAAGAGGGAGAGTCAAACCCGGATAAGAAGAACACGCCTGTT CAGAGTCCAGTGTCTCTGGGAGAAGACTTACAGTGGTGGCCTGATAAGGATGGAACAAAATTTACCTGCA TTGGGGCTCTGTATTCTGAGCTTCTTGCTGTCAGCAGCAAAGGAGAACTTTATCAATGGAAATGGAGTGA ATCTGAGCCTTACAGAAATGCCCAGAATCCTTCATTGCATCATCCACGAGCAACATTTTTGGGGTTGACC AATGAAAAGATAGTCCTCTTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACTGAAAATAACAAGGTTG CTACATGGGTGGATGAAACATTAAGTTCTGTGGCTTCTAAGTTAGAACACACAGCACAGACTTACTCTGA ACTTCAAGGAGAACGGATAGTTTCTTTACATTGCTGTGCCCTTTATACCTGTGCCCAGCTGGAGAATAAT TTGTATTGGTGGGGTGTTGTTCCTTTCAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATA AAAAGCCCAAATCCAGTGCTGGTATTTCTTCCATGCCAAACATCACTGTTGGAACTCAGGTGTGCTTGAG AAATAACCCTCTCTACCATGCTGGAGCGGTTGCCTTTTCAATTAGTGCTGGCATTCCTAAAGTTGGTGTC TTGATGGAGTCAGTATGGAATATGAATGATAGCTGTAGATTTCAACTTCGGTCTCCAGAGAGCCTGAAGA GCATGGAAAAAGCCAGCAAAACTCTTGAGACAAAGCCCGAGAGTAAGCAGGAACCAGTAAAAACAGAAAT GGGCCCTCCACCATCACCAGCATCCACCTGCAGCGACGCGTCCTCAATTGCCAGCAGTGCATCCATGCCA TACAAACGACGTCGGTCAACTCCTGCCCCGAGAGAAGAGGAGAAGGTGAACGAGGAGCAGTGGCCCCTTC GGGAAGTTGTTTTTGTTGAAGATGTTAAGAACGTTCCTGTTGGCAAGGTGCTGAAGGTAGATGGTGCCTA TGTCGCTGTAAAGTTTCCAGGAACATCTACTAACACCACCTGTCAGAACAGCTCTGGTCCAGACGCCGAC CCTTCCTCGCTCCTGCAGGACTGCAGGCTGCTTCGAATTGATGAGCTGCAGGTTGTCAAAACGGGCGGCA CCCCAAAAGTTCCCGACTGTTTTCAGAGGACTCCCAAAAAGCTTTGTATACCCGAGAAGACTGAAATACT AGCAGTAAATGTGGATTCCAAAGGTGTCCATGCTGTTCTGAAGACTGGAAGCTGGGTTCGGTACTGTGTC TTTGATCTTGCCACAGGGAAGGCTGAACAAGAGAATAATTTCCCAACAAGCAGTGTTGCTTTTCTTGGTC AGGATGAGAGGAGTGTCGCTATCTTCACTGCTGGGCAGGAATCTCCTATTGTTCTTCGAGATGGAAATGG TACTATCTACCCAATGGCCAAGGATTGCATGGGAGGAATACGGGATCCTGACTGGCTTGATCTCCCACCA ATCAGTAGTCTTGGGATGGGTGTACATTCTTTAATAAATCTTCCTGCCAACTCAACAATCAAAAAGAAAG CAGCTATTATCATCATGGCTGTAGAGAAGCAAACCTTAATGCAGCACATTCTCAGATGTGACTACGAGGC CTGCCGACAGTATCTCGTGAATCTGGAGCAAGCCGTGGTTTTAGAGCAGAACCTGCAGATGTTACAGACG TTCATCAGCCACAGATGTGATGGGAACCGGAACATTTTACATGCTTGTGTATCAGTTTGCTTTCCAACCA GCAATAAGGAAACTAAAGAAGAAGAGGAAGCTGAGCGCTCAGAAAGAAATACATTTGCTGAAAGGCTTTC TGCTGTGGAGGCCATTGCAAATGCAATATCAGTTGTTTCAAGTAACGGCCCAGGTAATCGGGCTGGATCA TCAAATAGTCGAAGTTTGAGATTACGAGAAATGATGAGACGTTCATTGAGAGCAGCTGGTTTGGGTAGAC ATGAGGCTGGAGCTTCATCCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGCTGGGT TCCTGACCCTCCTTCAATGGATCCTGATGGTGATATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTT ACCACAGCAGCGACTGGCAGTGGTCAAGGACCCAGCACCTCCACCATTCCAGGTCCTTCCACAGAGCCAT CTGTAGTAGAATCCAAGGATCGAAAGGCAAACGCACATTTCATATTAAAATTACTATGCGACAGTGCGGT TCTGCAGCCTTATCTACGAGAGCTTCTTTCTGCCAAGGATGCAAGAGGAATGACCCCGTTTATGTCAGCT GTAAGTGGCCGGGCTTATCCTGCTGCAATCACCATCCTAGAAACAGCCCAGAAGATTGCCAAAGCTGAAG TATCTGCAAGTGAAAAGGAGGAAGATGTGTTCATGGGAATGGTTTGCCCGTCAGGTACCAACCCTGACGA CTCCCCTTTGTATGTCTTATGTTGCAACGACACCTGCAGCTTTACCTGGACTGGAGCAGAGCACATTAAC CAGGACATTTTTGAATGTCGGACTTGTGGTTTACTGGAGTCGCTTTGTTGTTGTACAGAATGTGCAAGGG TTTGTCATAAAGGGCACGATTGCAAACTCAAGAGGACTTCACCAACAGCTTATTGTGATTGTTGGGAGAA ATGTAAATGTAAAACATTGATTGCTGGACAGAAGTCTGCTCGTCTTGATCTACTTTATCGCCTGCTTACT GCTACAAATCTGGTCACCCTACCAAACAGTAGGGGAGAGCACCTTTTGCTGTTCTTAGTACAGACCGTGG CCAGGCAGACAGTGGAGCACTGTCAGTATAGGCCTCCACGAATCAGGGAGGATCGCAACAGGAAAACAGC CAGTCCTGAAGATTCAGATATGCCTGACCATGACTTAGAGCCCCCAAGGTTTGCCCAGCTTGCCCTAGAG CGCGTCCTTCAGGACTGGAATGCTTTGAGATCTATGATTATGTTTGGGTCACAAGAGAACAAAGATCCTC TGAGTGCCAGCAGCAGGATAGGCCATCTTTTGCCAGAAGAGCAGGTCTACCTCAACCAGCAGAGCGGCAC GATTCGGCTCGACTGTTTCACTCATTGCCTTATTGTCAAGTGTACAGCAGATATTTTGCTTTTAGATACT CTGCTAGGCACCTTAGTAAAAGAACTCCAAAACAAGTACACACCCGGACGCCGGGAAGAAGCAATTGCCG TGACAATGAGGTTTCTACGATCTGTGGCAAGAGTGTTTGTCATTCTTAGTGTGGAAATGGCTTCGTCCAA AAAGAAAAACAACTTTATTCCACAGCCAATTGGAAAATGCAAACGTGTGTTTCAAGCACTGCTTCCTTAT GCTGTAGAAGAATTGTGTAATGTGGCAGAGTCTCTGATTGTTCCTGTCAGAATGGGGATTGCCCGGCCGA CAGCACCGTTTACTCTGGCCAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTAGA ACCATTACCACCACGGCCGTCATCAGATCAGGCAAGCAGCTCCAGTCAGTCTCAGTCATCTTACATCATT CGGAACCCACAGCAGAGGCGCATCAGCCAGTCACAGCCCGTCAGAGGCCGAGACGAGGAGCAGGATGATA TTGTGTCAGCAGACGTGGAAGAGGTTGAGGTGGTGGAAGGCGTGGCTGGAGAGGAGGATCATCATGATGA ACAGGAAGAGCACGGGGAAGAGAATGCTGAGGCTGAGGGACATCACGATGAGCATGATGAAGACGGAAGT GACATGGAGCTAGATTTGTTAGCAGCAGCTGAAACGGAAAGTGACAGTGAAAGTAACCATAGTAACCAAG ATAATGCTAGTGGGCGCAGAAGTGTTGTCACCGCAGCCACTGCTGGCTCAGAAGCAGGAGCGAGCAGTGT TCCTGCCTTTTTTTCTGAAGATGACTCTCAGTCAAATGACTCAAGTGACTCTGACAGCAGTAGCAGTCAG AGTGATGACATAGAGCAGGAGACCTTTATGCTCGATGAGCCACTAGAAAGGACAACGAATAGCTCCCATG CAAATGGTGCTGCCCAGGCTCCTCGCTCCATGCAGTGGGCTGTCCGAAATCCGCAGCATCAGCGAGCAGC AAGCACAGCTCCGTCCAGCACATCTACGCCAGCAGCAAGTTCTGCAGGTTTGATTTATATTGATCCTTCA AATTTACGCCGGAGCGGCACCATCAGTACAAGTGCTGCAGCGGCAGCAGCTGCTTTGGAAGCTAGCAATG CCAGCAGCTACTTAACATCTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATCAGACAAATCTCGGA CTTGATGGGCCTTATCCCTAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACT TACCAAGATGCAGTCAACTTACAGAACTATGTAGAAGAAAAGCTTATTCCTACGTGGAACTGGATGGTCA GTGTTATGGATTCTACTGAAGCTCAATTACGTTATGGTTCTGCACTAGCATCTGCTGGTGACCCAGGACA CCCAAATCATCCCCTTCATGCCTCTCAGAATTCAGCTCGAAGAGAGAGGATGACTGCACGGGAAGAGGCC AGTCTAAGAACGCTTGAAGGCAGACGACGTGCTACATTGCTGAGCGCCCGCCAGGGAATGATGTCTGCTC GAGGAGACTTTCTCAATTATGCTCTGTCTTTAATGAGATCTCATAATGATGAGCATTCTGATGTTCTTCC AGTATTGGATGTGTGCTCATTGAAACATGTGGCATATGTTTTCCAAGCCCTTATATACTGGATTAAAGCA ATGAATCAGCAGACAACATTAGACACTCCTCAACTGGAACGCAAAAGGACACGAGAACTTCTGGAACTGG GTATTGATAATGAAGATTCAGAACATGAAAATGATGATGACACCAGTCAAAGTGCTACTTTGAATGATAA GGATGACGACTCTCTTCCTGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCTATGACA TTCCTTGGATGTATACCTCCAAATCCATTTGAAGTGCCTTTGGCTGAAGCCATACCCTTGGCTGATCAGC CACATTTGTTGCAGCCAAATGCTAGAAAGGAAGATCTTTTTGGTCGCCCAAGTCAGGGTCTTTATTCCTC ATCTGCCGGAAGTGGGAAATGCATAGTAGAGGTTACAATGGACAGAAATTGTCTCGAGGTTCTTCCAACT AAAATGTCTTATGCTGCCAATCTAAAAAATGTAATGAACATGCAAAACCGGCAGAAGAAAGAAGGGGAAG AACAGAGCCTGCTGGCCGAAGAAGCTGACAGTTCAAAGCCAGGTCCATCCGCTCCTGATGTTGCTGCCCA GTTGAAAAGCAGTTTACTAGCAGAGATAGGACTCACGGAAAGTGAAGGGCCCCCCCTTACATCTTTCAGG CCTCAGTGCAGTTTCATGGGCATGGTTATCTCCCATGACATGCTGCTGGGACGCTGGCGCCTGTCTTTAG AACTGTTCGGCAGAGTATTCATGGAAGATGTTGGTGCAGAGCCTGGATCGATTTTAACTGAATTGGGTGG TTTTGAGGTAAAAGAATCAAAGTTCCGTAGAGAGATGGAAAAACTGAGAAACCAGCAATCAAGAGATTTA TCACTAGAGGTCGATCGCGATCGGGATCTTCTCATTCAGCAGACTATGAGACAGCTTAACAACCATTTTG GCCGAAGATGTGCTACCACGCCAATGGCTGTGCACAGAGTCAAAGTCACCTTTAAGGATGAACCAGGAGA GGGCAGTGGTGTAGCAAGAAGTTTCTATACAGCCATTGCACAAGCGTTTTTATCAAATGAAAAATTGCCA AATCTAGATTGTATCCAAAATGCCAACAAAGGTACTCATACAAGTTTAATGCAAAGATTGAGGAACAGAG GTGAAAGAGACCGGGAGAGAGAGAGAGAACGGGAAATGAGGAGGAGCAGTGGCTTGCGTGCAGGATCTCG AAGAGACAGGGATAGAGACTTCAGGAGACAGCTCTCCATTGACACCAGGCCTTTCAGACCTGCTTCTGAG GGGAATCCTAGTGATGACCCTGACCCTCTTCCAGCTCATCGGCAGGCACTTGGAGAGAGGCTTTACCCCC GAGTGCAAGCAATGCAGCCCGCATTTGCAAGTAAAATCACTGGCATGCTGTTGGAGTTATCCCCAGCTCA GCTGCTTCTCCTTCTAGCAAGTGAAGATTCTCTGAGGGCGAGAGTCGACGAGGCCATGGAACTCATTATT GCTCATGGACGGGAAAATGGAGCTGACAGTATTTTGGACCTCGGATTATTAGACTCTTCAGAAAAGGTAC AGGAAAACAGAAAGCGCCATGGCTCTAGTAGAAGTGTAGTGGATATGGATTTAGAGGACACAGATGATGG TGATGACAACGCTCCCTTGTTTTACCAACCTGGGAAGAGAGGATTTTATACTCCGAGACCTGGCAAAAAC ACAGAAGCAAGGCTGAATTGTTTCAGAAACATCGGCAGGATTCTTGGACTATGTCTGTTGCAAAATGAAC TATGTCCTATCACATTGAATAGGCATGTAATTAAAGTGTTGCTTGGTAGAAAAGTCAACTGGCATGACTT TGCTTTTTTCGACCCTGTAATGTATGAGAGTTTACGACAACTGATCCTTGCATCTCAGAGTTCAGATGCT GATGCTGTTTTCTCAGCAATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAGGGTGGAGGACAGG TTGAACTTATTCCAAATGGTGTAAATATACCAGTCACTCCGCAGAATGTATATGAGTATGTAAGGAAATA TGCAGAGCATAGAATGTTGGTGGTTGCAGAACAGCCATTGCACGCAATGAGAAAGGGTCTACTAGACGTG CTTCCAAAAAATTCCTTAGAAGACTTAACAGCTGAGGACTTCCGGCTCTTGGTGAACGGCTGTGGTGAAG TCAATGTGCAGATGCTGATCAGTTTCACTTCTTTCAATGATGAGTCAGGAGAAAATGCTGAGAAGCTCCT CCAGTTCAAACGGTGGTTCTGGTCAATAGTAGAGAAAATGAGCATGACAGAACGGCAGGACCTGGTTTAC TTCTGGACATCTAGCCCATCATTGCCAGCCAGTGAAGAAGGTTTTCAGCCTATGCCCTCGATCACAATAA GACCACCAGATGACCAACATCTTCCTACTGCAAATACTTGTATTTCTCGACTTTATGTCCCACTCTATTC CTCTAAACAGATTCTCAAACAAAAATTACTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220944 representing NM_001081359
Red=Cloning site Green=Tags(s) MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLNSHPPLNVLEQATIKQCVVGPNHAAFLLEDGRICRI GFSVQPDRLELGKPDNNDGSKLNSSSGTGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSG GGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQ RTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGY PSFRRSSLSRLGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGESNPDKKNTPV QSPVSLGEDLQWWPDKDGTKFTCIGALYSELLAVSSKGELYQWKWSESEPYRNAQNPSLHHPRATFLGLT NEKIVLLSANSIRATVATENNKVATWVDETLSSVASKLEHTAQTYSELQGERIVSLHCCALYTCAQLENN LYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGV LMESVWNMNDSCRFQLRSPESLKSMEKASKTLETKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMP YKRRRSTPAPREEEKVNEEQWPLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSTNTTCQNSSGPDAD PSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGSWVRYCV FDLATGKAEQENNFPTSSVAFLGQDERSVAIFTAGQESPIVLRDGNGTIYPMAKDCMGGIRDPDWLDLPP ISSLGMGVHSLINLPANSTIKKKAAIIIMAVEKQTLMQHILRCDYEACRQYLVNLEQAVVLEQNLQMLQT FISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGS SNSRSLRLREMMRRSLRAAGLGRHEAGASSSDHQDPVSPPIAPPSWVPDPPSMDPDGDIDFILAPAVGSL TTAATGSGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSAVLQPYLRELLSAKDARGMTPFMSA VSGRAYPAAITILETAQKIAKAEVSASEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHIN QDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLT ATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPEDSDMPDHDLEPPRFAQLALE RVLQDWNALRSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDT LLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPY AVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQASSSSQSQSSYII RNPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGHHDEHDEDGS DMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQ SDDIEQETFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNPQHQRAASTAPSSTSTPAASSAGLIYIDPS NLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLT YQDAVNLQNYVEEKLIPTWNWMVSVMDSTEAQLRYGSALASAGDPGHPNHPLHASQNSARRERMTAREEA SLRTLEGRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKA MNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTSQSATLNDKDDDSLPAETGQNHPFFRRSDSMT FLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSAGSGKCIVEVTMDRNCLEVLPT KMSYAANLKNVMNMQNRQKKEGEEQSLLAEEADSSKPGPSAPDVAAQLKSSLLAEIGLTESEGPPLTSFR PQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDL SLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLP NLDCIQNANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASE GNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELII AHGRENGADSILDLGLLDSSEKVQENRKRHGSSRSVVDMDLEDTDDGDDNAPLFYQPGKRGFYTPRPGKN TEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDA DAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDV LPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVY FWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081359 |
| ORF Size | 8394 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081359.3, NP_001074828.2 |
| RefSeq Size | 9790 bp |
| RefSeq ORF | 8397 bp |
| Locus ID | 70790 |
| UniProt ID | Q80TP3 |
| Gene Summary | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes (By similarity). Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Plays an essential role in extraembryonic development. Regulates DNA topoisomerase II binding protein (TopBP1) for the DNA damage response.[UniProtKB/Swiss-Prot Function] |
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