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Dgki (NM_001081206) Mouse Tagged ORF Clone
CAT＃: MG221210
- TrueORF®
Dgki (tGFP-tagged) - Mouse diacylglycerol kinase iota (Dgki), (10ug)
ORF Plasmid: DDK
"NM_001081206" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C130010K08Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221210 representing NM_001081206
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG CCGCCTCCAGCGCCCTCAGCCCGACCGGCCTCTGCAGCGGCACCACCTCGGCTTCTTTCGCGGCCGCCGG AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGTGGCAGCAGCAGCAGC GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGCGGACCCGCGGGGTGCAGGGGCAGCGG CTGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG GCACCTGCACATCCTCTTGGTCTTCCTGTGGCTAACGGTCCAGCGAAGGAGCCCAGAGCCACTCTGGACT GGAGCGAGAATGCTGTGAATGGGGAGCACCTGTGGCTGGAGACCAACGTTTCAGGGGACCTCTGCTACCT GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCCGTCTGCAAAATC GTGGTCCACACAGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCAACATTTCGGGAAG GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGTCACCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCATATTGAGGAACCCT GCTCCCTGGGGGCTCACGCCGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA CTCCCTGAAGGCTTCCAACCGGAAGAAGAAGAGGACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACT GAGCAGGAAACCAAAGGCCGTCCTTTTGTGATCAAGCCCATCTCCTCCCCTCTCATGAAGCCTTTGCTTG TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA TCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGATGTACAGGAAGGTACCA AATCTGAGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCACTGGGGACTGGGAATGACCTGGCTCGGACGCTCAA CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGATGGGACCATTGTA CAGTTGGATCGCTGGAACCTCCACGTAGAGAGAAACCCAGACCTGCCGCCCGAAGAACTTGAAGATGGCG TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGATGCTCACGTCACATTGGA GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCA GGGGCAGCCTTTTCTGATTTCCTACAAAGAAGTTCTAGAGATCTATCCAAACACGTCAAAGTTGTCTGTG ATGGGACAGACCTCACCCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAACGTCATGAT GACGGCTACATTGAAGTCATTGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTTGGGGGGCACGGAG AAAGGCTGCACCAGTGTCGGGAAGTGATGCTTCTGACTTACAAGTCCATTCCCATGCAAGTGGACGGGGA GCCATGTCGGTTGGCACCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC AAGCGGAGAACCTCGATGCCTTTGCTCAATGACATCCATCAGGTGCAAGCTGCGGACCTGCGGCGAGTGT CTGCTCCCCCCGGCTCCTTCACCATTCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGTGTGAACAAAAT CAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGACAAACTCCGGGAAGCTTCCATCCCTCTGGGT ATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGTCTACAGGAGGACT TGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACCTCCTTCCCCAGGGCACT CTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGATGCAACGTCTGCTGACCGCTTTTATCGAATA GACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCACGATGAAATTTTTATTCTGGACCCAG ATATGGTGGTGTCACAGCAGGCAGGGACCCCTCCAGGAATGCCTGACCTGGTGGTTGAACAGGCTTCTGG GCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCACACCCCAC TATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCCCCAGTCTCTTCAGAAGATC ATGCAATTTTGCAGGCAGTCCTCACCGGTGACCTTATGAAGCTCATGGAGAGCTATAAGAATGGAGGGAG CTTACTAATTCAGGGACCAGGGCACTGTTCTCTCCTTCACTACGCAGCTAAGACCGGCAATGGGGACATT GTGAAGTACATCCTTGACCACGGACCTGCAGAATTACTGGATATGGCAGACAGTGAAACGGGCGAGACTG CACTGCACAAGGCTGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGATGCAGGAGCATCTCT GAGACAGACAGACTCCAAGGGCAAGACACCCCAGGAACGAGCACAGCAGGCCGGGGACCCAGATTTGGCT GCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCATGAAGACCTGGAAACTGCTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221210 representing NM_001081206
Red=Cloning site Green=Tags(s) MDAAGRGCHLLPLPAARGPARAPAASSALSPTGLCSGTTSASFAAAGAVAMNPSSSAGEERGATGGSSSS GSGAGSCCLGAEGGADPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHL APAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKI VVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAIS CSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGT EQETKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALEMYRKVP NLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIV QLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYA GAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHD DGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKS KRRTSMPLLNDIHQVQAADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGLHYDKDKLREASIPLG ILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDQETSFPRALSAQRLSPRWCFLDATSADRFYRI DRSQEHLHFVMEISHDEIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPH YEDSDLKDFSHSRVLQSPVSSEDHAILQAVLTGDLMKLMESYKNGGSLLIQGPGHCSLLHYAAKTGNGDI VKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRQTDSKGKTPQERAQQAGDPDLA AYLESRQNYKIIGHEDLETAV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081206 |
| ORF Size | 3213 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081206.2 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 3216 bp |
| Locus ID | 320127 |
| UniProt ID | D3YWQ0 |
| Gene Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position (By similarity). By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway (PubMed:15894621). May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (PubMed:21119615).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223463 | Dgki (untagged) - Mouse diacylglycerol kinase, iota (Dgki), (10ug) |
CNY 13,780.00 |
|
| MR221210 | Dgki (Myc-DDK-tagged) - Mouse diacylglycerol kinase, iota (Dgki) |
CNY 7,456.00 |
|
| MR221210L3 | Lenti ORF clone of Dgki (Myc-DDK-tagged) - Mouse diacylglycerol kinase, iota (Dgki) |
CNY 10,260.00 |
|
| MR221210L4 | Lenti ORF clone of Dgki (mGFP-tagged) - Mouse diacylglycerol kinase, iota (Dgki) |
CNY 10,260.00 |
