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St14 (NM_011176) Mouse Tagged ORF Clone
CAT＃: MG222240
- TrueORF®
St14 (tGFP-tagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14), (10ug)
ORF Plasmid: DDK
"NM_011176" in other vectors (4)
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CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Epithin; matriptase; mCAP3; MT-SP1; Prss14; Tmprss14 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222240 representing NM_011176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAGCAATCGGGGCCGCAAGGCCGGAGGGGGCTCTCAGGACTTCGGCGCGGGACTCAAGTACAACT CCCGGCTAGAGAACATGAATGGCTTTGAGGAGGGTGTGGAGTTCCTGCCTGCGAACAATGCCAAGAAAGT GGAGAAGCGAGGCCCCAGGCGCTGGGTGGTGCTGGTGGCAGTGCTGTTCAGCTTCCTCTTGCTCTCCCTC ATGGCTGGCTTGCTGGTGTGGCACTTCCATTATCGGAATGTGCGGGTTCAAAAAGTCTTCAATGGCCATC TGAGGATCACAAATGAGATCTTTCTGGATGCGTATGAGAACTCCACCTCCACAGAGTTTATCAGCCTGGC CAGCCAGGTGAAGGAGGCGCTGAAGCTGCTGTACAATGAAGTCCCTGTCCTGGGTCCCTACCACAAGAAG TCGGCTGTAACTGCCTTCAGTGAGGGCAGTGTCATCGCCTACTACTGGTCAGAGTTCAGCATCCCCCCAC ACCTGGCAGAAGAGGTTGATCGCGCCATGGCTGTGGAGCGAGTTGTAACATTGCCACCCCGAGCACGGGC ACTGAAATCCTTCGTGCTAACATCTGTGGTGGCCTTCCCCATTGACCCCAGAATGCTGCAGAGGACTCAG GACAACAGCTGCAGTTTTGCCCTGCATGCCCATGGTGCAGCAGTGACACGCTTCACTACCCCTGGCTTCC CCAACAGTCCCTACCCGGCGCATGCCCGCTGCCAGTGGGTCCTGCGGGGGGACGCCGACTCTGTGCTGAG CCTCACCTTCCGAAGCTTTGATGTCGCTCCCTGTGATGAGCATGGCAGTGACCTGGTCACCGTGTATGAT AGCCTGAGCCCCATGGAACCCCACGCTGTGGTGCGGCTGTGTGGCACCTTCTCACCCTCCTACAACCTGA CTTTCCTCTCCTCCCAGAACGTCTTCCTTGTCACGCTGATAACCAATACTGACCGGCGACATCCTGGCTT TGAGGCCACTTTCTTCCAGCTGCCCAAGATGAGCAGCTGTGGCGGCTTTTTGAGTGACACCCAAGGGACA TTTAGCAGCCCCTACTATCCAGGCCACTACCCGCCCAACATCAACTGCACATGGAATATCAAGGTGCCCA ACAACCGGAACGTGAAGGTGCGCTTCAAACTCTTCTATCTGGTGGACCCCAACGTACCAGTGGGCTCCTG CACCAAGGACTATGTGGAGATCAACGGGGAGAAGTACTGCGGTGAGAGGTCCCAGTTTGTGGTGAGCAGC AACAGCAGCAAGATTACAGTCCACTTCCATTCTGATCACTCGTACACGGACACCGGGTTCCTAGCTGAGT ACCTCTCCTACGACTCCAACGACCCGTGCCCAGGGATGTTCATGTGCAAGACTGGACGGTGCATCCGAAA GGAACTGCGCTGCGACGGCTGGGCAGACTGCCCGGATTATAGTGATGAGCGTTACTGCCGATGCAATGCC ACCCACCAGTTCACGTGCAAAAACCAGTTCTGCAAGCCCCTCTTCTGGGTCTGTGACAGTGTCAACGACT GTGGGGACGGAAGTGACGAGGAGGGCTGCAGCTGTCCTGCTGGGAGTTTCAAGTGTTCCAATGGGAAGTG TCTCCCTCAGAGCCAGAAGTGTAATGGGAAGGACAACTGTGGAGATGGGTCTGACGAGGCTTCATGTGAC AGCGTGAATGTCGTCTCTTGCACCAAATATACCTACCGCTGCCAAAATGGCCTCTGTCTGAGCAAGGGCA ACCCTGAGTGTGATGGGAAGACGGACTGTAGCGATGGCTCCGATGAGAAAAACTGTGACTGTGGGCTGCG ATCCTTTACCAAACAGGCTCGCGTGGTTGGTGGCACGAATGCGGACGAGGGCGAGTGGCCCTGGCAGGTG AGCCTCCACGCCCTGGGCCAGGGCCACTTGTGTGGGGCCTCGCTCATCTCTCCTGACTGGCTGGTCTCTG CAGCTCATTGCTTTCAGGATGACAAAAATTTCAAGTACTCAGACTACACGATGTGGACGGCCTTCCTGGG TCTGCTGGACCAGAGCAAGCGCAGTGCCTCTGGGGTGCAGGAGCTGAAGCTCAAACGTATCATCACCCAC CCTTCCTTCAATGATTTCACCTTCGACTATGACATCGCCTTGCTGGAGCTGGAGAAGTCGGTGGAGTACA GCACCGTCGTGCGCCCCATCTGCCTGCCTGATGCTACCCATGTCTTCCCTGCTGGCAAGGCCATCTGGGT CACAGGCTGGGGGCACACAAAAGAGGGAGGTACCGGAGCGCTGATCCTGCAGAAGGGTGAGATCCGTGTC ATCAACCAGACCACCTGTGAGGACCTCATGCCGCAGCAGATCACCCCACGAATGATGTGTGTGGGTTTCC TCAGTGGGGGTGTGGACTCCTGCCAGGGTGACTCTGGTGGCCCCTTGTCAAGCGCGGAGAAAGATGGGCG AATGTTCCAGGCTGGTGTGGTGAGCTGGGGTGAAGGCTGCGCTCAGAGGAACAAGCCAGGCGTGTACACA AGGCTCCCTGTAGTTCGGGACTGGATCAAAGAGCACACTGGGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222240 representing NM_011176
Red=Cloning site Green=Tags(s) MGSNRGRKAGGGSQDFGAGLKYNSRLENMNGFEEGVEFLPANNAKKVEKRGPRRWVVLVAVLFSFLLLSL MAGLLVWHFHYRNVRVQKVFNGHLRITNEIFLDAYENSTSTEFISLASQVKEALKLLYNEVPVLGPYHKK SAVTAFSEGSVIAYYWSEFSIPPHLAEEVDRAMAVERVVTLPPRARALKSFVLTSVVAFPIDPRMLQRTQ DNSCSFALHAHGAAVTRFTTPGFPNSPYPAHARCQWVLRGDADSVLSLTFRSFDVAPCDEHGSDLVTVYD SLSPMEPHAVVRLCGTFSPSYNLTFLSSQNVFLVTLITNTDRRHPGFEATFFQLPKMSSCGGFLSDTQGT FSSPYYPGHYPPNINCTWNIKVPNNRNVKVRFKLFYLVDPNVPVGSCTKDYVEINGEKYCGERSQFVVSS NSSKITVHFHSDHSYTDTGFLAEYLSYDSNDPCPGMFMCKTGRCIRKELRCDGWADCPDYSDERYCRCNA THQFTCKNQFCKPLFWVCDSVNDCGDGSDEEGCSCPAGSFKCSNGKCLPQSQKCNGKDNCGDGSDEASCD SVNVVSCTKYTYRCQNGLCLSKGNPECDGKTDCSDGSDEKNCDCGLRSFTKQARVVGGTNADEGEWPWQV SLHALGQGHLCGASLISPDWLVSAAHCFQDDKNFKYSDYTMWTAFLGLLDQSKRSASGVQELKLKRIITH PSFNDFTFDYDIALLELEKSVEYSTVVRPICLPDATHVFPAGKAIWVTGWGHTKEGGTGALILQKGEIRV INQTTCEDLMPQQITPRMMCVGFLSGGVDSCQGDSGGPLSSAEKDGRMFQAGVVSWGEGCAQRNKPGVYT RLPVVRDWIKEHTGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011176 |
| ORF Size | 2565 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011176.4, NP_035306.2 |
| RefSeq Size | 4017 bp |
| RefSeq ORF | 2568 bp |
| Locus ID | 19143 |
| UniProt ID | P56677 |
| Gene Summary | Degrades extracellular matrix. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site (By similarity). Involved in the terminal differentiation of keratinocytes through prostasin (PRSS8) activation and filaggrin (FLG) processing (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222151 | St14 (untagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14), (10ug) |
CNY 10,930.00 |
|
| MR222240 | St14 (Myc-DDK-tagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14) |
CNY 6,264.00 |
|
| MR222240L3 | Lenti ORF clone of St14 (Myc-DDK-tagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14) |
CNY 9,410.00 |
|
| MR222240L4 | Lenti ORF clone of St14 (mGFP-tagged) - Mouse suppression of tumorigenicity 14 (colon carcinoma) (St14) |
CNY 9,410.00 |
