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Kdm5a (NM_145997) Mouse Tagged ORF Clone
CAT＃: MG223212
- TrueORF®
Kdm5a (tGFP-tagged) - Mouse lysine (K)-specific demethylase 5A (Kdm5a), (10ug)
ORF Plasmid: DDK
"NM_145997" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409370; C76986; Jarid1a; Rbbp2; RBP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG223212 representing NM_145997
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCCGAGTGCCCGGTCTTTGAGC CCAGTTGGGAGGAGTTCACAGACCCGCTGAGTTTTATCGGGCGCATCCGGCCCTTTGCGGAGAAAACGGG CATCTGCAAAATCCGGCCACCCAAGGATTGGCAACCTCCATTTGCCTGTGAAGTAAAAACCTTCCGTTTC ACTCCTAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGATCAAC TGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATCCCTGTGGTGGAGAGAAAAATCCTGGATTT GTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAATGGTCT AAAGTGGGTAGCCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACACTATGAAA GAATTCTCTACCCATATGAACTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCTGATTTAGA TCTTAAAGAAAAAGTTGAGGCTGAGGTTCTCAGCACTGACATCCAGCCTTCCCCAGAGCGGGGAACAAGA ATGAATATTCCACCAAAGAGAACAAGACGAGTTAAGTCTCAGTCAGACTCTGGAGAAGTAAATAGAAACA CAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTTGTGGGCCTGGCAGTAGGAGCAAAAGA TAAAGAAGATGAAGTCACCCGAAGAAGAAAAGTTACCAACAGGTCAGATGCATTTAACATGCAAATGAGA CAACGGAAAGGAACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGCATGTTTTGTGGTCGAGGAAATA ATGAGGATAAACTGCTTTTGTGTGATGGGTGCGATGACAGCTATCATACATTTTGTCTACTTCCACCACT ACCTGATGTCCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGTAACAAACCTCGAGAA GCCTTTGGATTTGAGCAAGCTGTAAGAGAATATACACTTCAAAGCTTTGGAGAGATGGCAGATAATTTTA AATCTGATTATTTCAATATGCCAGTCCATATGGTTCCTACAGAGCTAGTAGAAAAAGAATTCTGGCGGCT AGTAAGTAGCATTGAAGAAGATGTTATTGTAGAGTATGGAGCTGATATCTCTTCAAAAGATTTTGGAAGT GGATTTCCTAAAAAGGATGGTCAAAGAAAGATGTTGCCAGAAGAAGAGGAATATGCACTTTCTGGTTGGA ATTTGAATAACATGCCTGTTCTGGAGCAGTCCGTTCTTGCACATATTAATGTGGACATTTCTGGTATGAA GGTGCCATGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCATATTGAAGATCACTGGAGTTAT TCCATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTACCATCTCATGCTGCAGAACAAC TGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCCCAGCCTGATCTCCTACATCAGTTAGT AACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTGTACAGGACTAATCAGTGTGCTGGA GAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTTGCCGAAGCGG TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGTGTAAATCACTATCGACGGCTCAGGCG CCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG CTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAAGAAGAAACACGATTGAGAGAATCAGTTG TTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTC AGCATGCAGAACTACATGTTTTCTCTCAGCTCTCACATGCTCCTGTAATCCGGAGCGACTTGTATGTCTG TATCATCCAACTGATTTGTGCTCTTGCCCCATGCAGAACAAATGTCTTAGGTACCGCTATCCACTAGAAG ACCTTCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCACAATCCTATGACACTTGGGTCAATCGTGTTAC AGAAGCATTGTCAGCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCT GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCT GTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAGCAAAAACACAGGCAGAGCTCAGATAGTGGGAAGAC TCGGACCAAACTGACAGTAGAAGAATTGAAGGCCTTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATC AGCCAAACTCGACAAGTAAAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCA TGATGGATGAAACCCCAGATTCTTCCAAACTCCAAATGTTAATAGACATGGGCTCTAGTCTCTATGTGGA GCTACCTGAATTGCCCCGACTAAAGCAAGAGCTCCAGCAGGCTCGATGGCTGGATGAAGTAAGGCTAACT CTATCAGATCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGCGTAGGCTTGGCAC CTCACCATGCTGTAGAAAAAGCAATGGCCGAACTACAGGAACTCCTTACAGTCTCTGAACGATGGGAAGA GAAGGCTAAGGTCTGCCTGCAAGCAAGACCTCGGCACAGTATGGCAAATTTAGAAAATATTGTGAATGAA GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAAT GGACAGCTAAAGTGGAAGCTATTCAGAGTGGCAACAACTATGCTTACTTGGAACAGCTTGAGAGCCTATC TGCTAAAGGACGCCCTATCCCTGTGCGTCTTGATGCCTTGCCTCAAGTGGAGTCACAGGTAGCAGCAGCA CGGGCCTGGAGAGAACGTACTGGGCGGACCTTCCTTAAAAAGAATTCCAGCCATACATTGTTACAGGTGC TAAGTCCACGAACTGACATTGGTGTATATGGGAGTGGTAAAAACAGGAGGAAAAAGGTAAAAGAAATAAT AGAAAAAGAAAAGGAGAAGGACCTCGACCTTGAGCCTTTGAGTGACTTAGAGGAAGGACTGGAGGAGTCA AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGAGAGCAAAAGGAGATTGAAGCCATGCATTCCC TCAGAGCAGCCAACCTAGCCAAGATGACAATTGTGGAGCGCATAGAAGAAGTGAAATTTTGTATTTGCCG CAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAACAGCTGTGTTCCC CTTCCTAAATCAAGTTCCCAGAAAAAGGGATCCAGCTGGCAGGCTAAAGATGTAAAATTCCTTTGCCCTC TTTGCATGAGATCTCGGAGGCCAAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC TGTACGGTTGCCGGAAGGAGAAGCACTGCAGTGTTTGACAGAACGTGCCATGAGTTGGCAAGATAAAGCA CGCCAAGCTTTAGCCACAGATGAACTGTCCTCTGCCCTGGCCAAGCTCTCTGTGCTAAGCCAGCGTATGG TGGAACAAGCAGCTAGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAAAAAGCAGCTGCCAATCC AGATTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCATCT TCCCCCCACCAAACAATGGACTATGATGATGAAGAAACAGATTCTGATGAAGATATTCGGGAGACATACG GCTATGACATGAAGGATACAGCCAGCGTGAAGTCCTCTAGTAGTCTGGAACCCAATCTCTTTTGTGATGA AGAGATTCCCATCAAGTCTGAGGAAGTGGTGACTCACATGTGGACGGCACCGTCATTCTGTGCAGAGCAT GCATATTCTTCTGCTTCAAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCC CTTTGGTTCCCAGAAGTTTGGAGCCTCCAGTATTGGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGA ACTCATGATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATTTGGCGGATTTTGCAG GCCACACATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCATAGAAGAGAAACCAT TAAAAATGAAAGGAAAGGACTCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAACAGCTTTT TGGAGAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTA AATGTGGACAAATCAAAAGAGCTGAATAAATTGGCCAAGAAGTTAGCTAAAGAAGAAGAGCGAAAGAAAA AGAAAGAAAAGGCTGCTGCCGCCAAAGTGGAGCTTGTGAAAGAGAGCACGGAGAAGAAACGGGAGAGGAA GGTGTTGGATATCCCCTCAAAGTATGACTGGTCGGGGGCAGAGGAGTCTGATGATGAGAATGCTGTGTGT GCAGCCCAAAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAGTGTGATGGTGGCTGTGATG AGTGGTTTCATCAAGTTTGTGTTGGTGTATCCGCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA CTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCAAGCACCACCTCCTCCCTTCCTAATGAGCTACAAA CTACCAATGGAGGATCTTAAAGAGACCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG223212 representing NM_145997
Red=Cloning site Green=Tags(s) MASVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPFAEKTGICKIRPPKDWQPPFACEVKTFRF TPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEIVTKEKKWS KVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPDLDLKEKVEAEVLSTDIQPSPERGTR MNIPPKRTRRVKSQSDSGEVNRNTELKKLQIFGAGPKVVGLAVGAKDKEDEVTRRRKVTNRSDAFNMQMR QRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLLPPLPDVPKGDWRCPKCVAEECNKPRE AFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGS GFPKKDGQRKMLPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSY SINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAG EFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVG LAAMVCKELTLMTEEETRLRESVVQMGVVMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCL YHPTDLCSCPMQNKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVNRVTEALSASFNHKKDLIELRVMLEDA EDRKYPENDLFRKLRDAVKEAETCGSVAQLLLSKKQKHRQSSDSGKTRTKLTVEELKAFVQQLVSLPCVI SQTRQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLT LSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSMANLENIVNE AKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGNNYAYLEQLESLSAKGRPIPVRLDALPQVESQVAAA RAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKEIIEKEKEKDLDLEPLSDLEEGLEES RDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTIVERIEEVKFCICRKTASGFMLQCELCKDWFHNSCVP LPKSSSQKKGSSWQAKDVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDKA RQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSS SPHQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEH AYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQ ATHPPSEDRFLHIMEDDSIEEKPLKMKGKDSSEKKRKRKLEKVEQLFGEGKQKTKELKKIDKPKKKKLKL NVDKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKRERKVLDIPSKYDWSGAEESDDENAVC AAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSAEMAENEDYICINCAKKQGPDSPGQAPPPPFLMSYK LPMEDLKETS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145997 |
| ORF Size | 5070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145997.2, NP_666109.2 |
| RefSeq Size | 10969 bp |
| RefSeq ORF | 5073 bp |
| Locus ID | 214899 |
| UniProt ID | Q3UXZ9 |
| Gene Summary | Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4' (PubMed:17320161, PubMed:17320163). Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (By similarity). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (PubMed:21960634). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] |
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