Kdm5a (NM_145997) Mouse Untagged Clone

CAT#: MC224835

Kdm5a (untagged) - Mouse lysine (K)-specific demethylase 5A (Kdm5a), (10ug)



  "NM_145997" in other vectors (2)

CNY 27,170.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA409370; C76986; Jarid1a; Rbbp2; RBP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224835 representing NM_145997
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCCGAGTGCCCGGTCTTTGAGC
CCAGTTGGGAGGAGTTCACAGACCCGCTGAGTTTTATCGGGCGCATCCGGCCCTTTGCGGAGAAAACGGG
CATCTGCAAAATCCGGCCACCCAAGGATTGGCAACCTCCATTTGCCTGTGAAGTAAAAACCTTCCGTTTC
ACTCCTAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGATCAAC
TGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATCCCTGTGGTGGAGAGAAAAATCCTGGATTT
GTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAATGGTCT
AAAGTGGGTAGCCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACACTATGAAA
GAATTCTCTACCCATATGAACTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCTGATTTAGA
TCTTAAAGAAAAAGTTGAGGCTGAGGTTCTCAGCACTGACATCCAGCCTTCCCCAGAGCGGGGAACAAGA
ATGAATATTCCACCAAAGAGAACAAGACGAGTTAAGTCTCAGTCAGACTCTGGAGAAGTAAATAGAAACA
CAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTTGTGGGCCTGGCAGTAGGAGCAAAAGA
TAAAGAAGATGAAGTCACCCGAAGAAGAAAAGTTACCAACAGGTCAGATGCATTTAACATGCAAATGAGA
CAACGGAAAGGAACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGCATGTTTTGTGGTCGAGGAAATA
ATGAGGATAAACTGCTTTTGTGTGATGGGTGCGATGACAGCTATCATACATTTTGTCTACTTCCACCACT
ACCTGATGTCCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGTAACAAACCTCGAGAA
GCCTTTGGATTTGAGCAAGCTGTAAGAGAATATACACTTCAAAGCTTTGGAGAGATGGCAGATAATTTTA
AATCTGATTATTTCAATATGCCAGTCCATATGGTTCCTACAGAGCTAGTAGAAAAAGAATTCTGGCGGCT
AGTAAGTAGCATTGAAGAAGATGTTATTGTAGAGTATGGAGCTGATATCTCTTCAAAAGATTTTGGAAGT
GGATTTCCTAAAAAGGATGGTCAAAGAAAGATGTTGCCAGAAGAAGAGGAATATGCACTTTCTGGTTGGA
ATTTGAATAACATGCCTGTTCTGGAGCAGTCCGTTCTTGCACATATTAATGTGGACATTTCTGGTATGAA
GGTGCCATGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCATATTGAAGATCACTGGAGTTAT
TCCATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTACCATCTCATGCTGCAGAACAAC
TGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCCCAGCCTGATCTCCTACATCAGTTAGT
AACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTGTACAGGACTAATCAGTGTGCTGGA
GAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTTGCCGAAGCGG
TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGTGTAAATCACTATCGACGGCTCAGGCG
CCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG
CTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAAGAAGAAACACGATTGAGAGAATCAGTTG
TTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTC
AGCATGCAGAACTACATGTTTTCTCTCAGCTCTCACATGCTCCTGTAATCCGGAGCGACTTGTATGTCTG
TATCATCCAACTGATTTGTGCTCTTGCCCCATGCAGAACAAATGTCTTAGGTACCGCTATCCACTAGAAG
ACCTTCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCACAATCCTATGACACTTGGGTCAATCGTGTTAC
AGAAGCATTGTCAGCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCT
GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGACGCGGTGAAAGAAGCTGAGACCT
GTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAGCAAAAACACAGGCAGAGCTCAGATAGTGGGAAGAC
TCGGACCAAACTGACAGTAGAAGAATTGAAGGCCTTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATC
AGCCAAACTCGACAAGTAAAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCA
TGATGGATGAAACCCCAGATTCTTCCAAACTCCAAATGTTAATAGACATGGGCTCTAGTCTCTATGTGGA
GCTACCTGAATTGCCCCGACTAAAGCAAGAGCTCCAGCAGGCTCGATGGCTGGATGAAGTAAGGCTAACT
CTATCAGATCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGCGTAGGCTTGGCAC
CTCACCATGCTGTAGAAAAAGCAATGGCCGAACTACAGGAACTCCTTACAGTCTCTGAACGATGGGAAGA
GAAGGCTAAGGTCTGCCTGCAAGCAAGACCTCGGCACAGTATGGCAAATTTAGAAAATATTGTGAATGAA
GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAAT
GGACAGCTAAAGTGGAAGCTATTCAGAGTGGCAACAACTATGCTTACTTGGAACAGCTTGAGAGCCTATC
TGCTAAAGGACGCCCTATCCCTGTGCGTCTTGATGCCTTGCCTCAAGTGGAGTCACAGGTAGCAGCAGCA
CGGGCCTGGAGAGAACGTACTGGGCGGACCTTCCTTAAAAAGAATTCCAGCCATACATTGTTACAGGTGC
TAAGTCCACGAACTGACATTGGTGTATATGGGAGTGGTAAAAACAGGAGGAAAAAGGTAAAAGAAATAAT
AGAAAAAGAAAAGGAGAAGGACCTCGACCTTGAGCCTTTGAGTGACTTAGAGGAAGGACTGGAGGAGTCA
AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGAGAGCAAAAGGAGATTGAAGCCATGCATTCCC
TCAGAGCAGCCAACCTAGCCAAGATGACAATTGTGGAGCGCATAGAAGAAGTGAAATTTTGTATTTGCCG
CAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAACAGCTGTGTTCCC
CTTCCTAAATCAAGTTCCCAGAAAAAGGGATCCAGCTGGCAGGCTAAAGATGTAAAATTCCTTTGCCCTC
TTTGCATGAGATCTCGGAGGCCAAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC
TGTACGGTTGCCGGAAGGAGAAGCACTGCAGTGTTTGACAGAACGTGCCATGAGTTGGCAAGATAAAGCA
CGCCAAGCTTTAGCCACAGATGAACTGTCCTCTGCCCTGGCCAAGCTCTCTGTGCTAAGCCAGCGTATGG
TGGAACAAGCAGCTAGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAAAAAGCAGCTGCCAATCC
AGATTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCATCT
TCCCCCCACCAAACAATGGACTATGATGATGAAGAAACAGATTCTGATGAAGATATTCGGGAGACATACG
GCTATGACATGAAGGATACAGCCAGCGTGAAGTCCTCTAGTAGTCTGGAACCCAATCTCTTTTGTGATGA
AGAGATTCCCATCAAGTCTGAGGAAGTGGTGACTCACATGTGGACGGCACCGTCATTCTGTGCAGAGCAT
GCATATTCTTCTGCTTCAAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCC
CTTTGGTTCCCAGAAGTTTGGAGCCTCCAGTATTGGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGA
ACTCATGATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATTTGGCGGATTTTGCAG
GCCACACATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCATAGAAGAGAAACCAT
TAAAAATGAAAGGAAAGGACTCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAACAGCTTTT
TGGAGAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTA
AATGTGGACAAATCAAAAGAGCTGAATAAATTGGCCAAGAAGTTAGCTAAAGAAGAAGAGCGAAAGAAAA
AGAAAGAAAAGGCTGCTGCCGCCAAAGTGGAGCTTGTGAAAGAGAGCACGGAGAAGAAACGGGAGAGGAA
GGTGTTGGATATCCCCTCAAAGTATGACTGGTCGGGGGCAGAGGAGTCTGATGATGAGAATGCTGTGTGT
GCAGCCCAAAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAGTGTGATGGTGGCTGTGATG
AGTGGTTTCATCAAGTTTGTGTTGGTGTATCCGCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA
CTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCAAGCACCACCTCCTCCCTTCCTAATGAGCTACAAA
CTACCAATGGAGGATCTTAAAGAGACCAGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145997
Insert Size 5073 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145997.2, NP_666109.2
RefSeq Size 10969 bp
RefSeq ORF 5073 bp
Locus ID 214899
UniProt ID Q3UXZ9
Gene Summary Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4' (PubMed:17320161, PubMed:17320163). Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation (By similarity). May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1 (PubMed:21960634). Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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