Sell (NM_001164059) Mouse Tagged ORF Clone

CAT#: MG225584

  • TrueORF®

Sell (tGFP-tagged) - Mouse selectin lymphocyte (Sell) transcript variant 2, (10ug)

ORF Plasmid: DDK tGFP



  "NM_001164059" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Recombinant Anti-CD62L (Clone MEL-14)
    • 200 ug

CNY 6,881.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI528707; CD62L; L-selectin; LECAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG225584 representing NM_001164059
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTTCCATGGAGATGTGAGGGTACTTACTGGGGCTCGAGGAACATCCTGAAGCTGTGGGTCTGGA
CACTGCTCTGTTGTGACTTCCTGATACACCATGGAACTCACTGTTGGACTTACCATTATTCTGAAAAGCC
CATGAACTGGGAAAATGCTAGAAAGTTCTGCAAGCAAAATTACACAGATTTAGTCGCCATACAAAACAAG
AGAGAAATTGAGTATTTAGAGAATACATTGCCCAAAAGCCCTTATTACTACTGGATAGGAATCAGGAAAA
TTGGGAAAATGTGGACATGGGTGGGAACCAACAAAACTCTCACTAAAGAAGCAGAGAACTGGGGTGCTGG
GGAGCCCAACAACAAGAAGTCCAAGGAGGACTGTGTGGAGATCTATATCAAGAGGGAACGAGACTCTGGG
AAATGGAACGATGACGCCTGTCACAAACGAAAGGCAGCTCTCTGCTACACAGTGGTCCAGTGTGAGCCTT
TGGAGGCCCCTGAGTTGGGTACCATGGACTGCATCCACCCCTTGGGAAACTTCAGCTTCCAGTCCAAGTG
TGCTTTCAACTGTTCTGAGGGAAGAGAGCTACTTGGGACTGCAGAAACACAGTGTGGAGCATCTGGAAAC
TGGTCATCTCCAGAGCCAATCTGCCAAGTGGTCCAGTGTGAGCCTTTGGAGGCCCCTGAGTTGGGTACCA
TGGACTGCATCCACCCCTTGGGAAACTTCAGCTTCCAGTCCAAGTGTGCTTTCAACTGTTCTGAGGGAAG
AGAGCTACTTGGGACTGCAGAAACACAGTGTGGAGCATCTGGAAACTGGTCATCTCCAGAGCCAATCTGC
CAAGAGACAAACAGAAGTTTCTCAAAGATCAAAGAAGGTGACTACAACCCCCTCTTCATTCCTGTAGCCG
TCATGGTCACCGCATTCTCGGGGCTGGCATTTCTCATTTGGCTGGCAAGGCGGTTAAAAAAAGGCAAGAA
ATCTCAAGAAAGGATGGATGATCCATAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG225584 representing NM_001164059
Red=Cloning site Green=Tags(s)

MVFPWRCEGTYWGSRNILKLWVWTLLCCDFLIHHGTHCWTYHYSEKPMNWENARKFCKQNYTDLVAIQNK
REIEYLENTLPKSPYYYWIGIRKIGKMWTWVGTNKTLTKEAENWGAGEPNNKKSKEDCVEIYIKRERDSG
KWNDDACHKRKAALCYTVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGN
WSSPEPICQVVQCEPLEAPELGTMDCIHPLGNFSFQSKCAFNCSEGRELLGTAETQCGASGNWSSPEPIC
QETNRSFSKIKEGDYNPLFIPVAVMVTAFSGLAFLIWLARRLKKGKKSQERMDDPY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001164059
ORF Size 1008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001164059.1, NP_001157531.1
RefSeq Size 2213 bp
RefSeq ORF 1011 bp
Locus ID 20343
Gene Summary Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes (PubMed:1693096). Promotes initial tethering and rolling of leukocytes in endothelia (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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