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Pla2g15 (NM_133792) Mouse Tagged ORF Clone
CAT＃: MG225901
- TrueORF®
Pla2g15 (tGFP-tagged) - Mouse phospholipase A2 group XV (Pla2g15), (10ug)
ORF Plasmid: DDK
"NM_133792" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ACS; C87498; LLPL; Lpla2; Lypla3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225901 representing NM_133792
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGCCATCTCTGCACCTGTCGCGAGACCCAGCTCCGGAGTGGCCTCCTGTTACCTCTGTTTCTAC TAATGATGCTGGCAGACCTGACGCTCCCGGCCCAACGTCACCCCCCGGTGGTGCTGGTGCCTGGTGATTT GGGTAACCAGTTGGAAGCAAAGCTGGATAAGCCAAAGGTTGTACACTACCTTTGCTCCAAGAAGACGGAC AGCTACTTCACACTCTGGCTGAATCTGGAACTGCTTCTGCCTGTTATCATTGACTGCTGGATTGACAATA TCAGGCTGGTTTACAACAGAACATCTCGGGCCACCCAGTTTCCCGATGGTGTGGACGTGCGTGTCCCTGG CTTTGGGGAAACATTTTCTATGGAATTCCTAGACCCCAGCAAGAGGAATGTGGGTTCCTATTTCTACACT ATGGTGGAGAGCCTTGTGGGCTGGGGCTACACACGGGGTGAAGACGTTCGAGGTGCTCCCTATGATTGGC GGCGAGCCCCAAATGAAAACGGGCCCTACTTCTTGGCCCTGCGAGAGATGATCGAGGAGATGTACCAGAT GTATGGGGGCCCCGTGGTGCTGGTCGCCCACAGCATGGGCAACGTGTACATGCTCTACTTTCTGCAGCGG CAGCCACAAGTCTGGAAGGACAAATATATCCATGCCTTCGTCTCACTGGGGGCGCCCTGGGGGGGCGTGG CCAAGACGCTGCGTGTCCTGGCCTCAGGAGACAACAATCGCATTCCCGTCATTGGGCCACTGAAGATCCG GGAACAGCAGCGATCTGCCGTCTCTACCAGCTGGCTACTGCCATACAACCACACTTGGTCACATGAAAAG GTATTTGTATACACACCCACGACTAACTACACGCTCCGGGACTATCACCGGTTCTTCCGGGACATCGGTT TCGAAGATGGCTGGTTCATGCGGCAGGACACAGAAGGGCTGGTTGAAGCCATGACGCCACCCGGGGTGGA GCTGCACTGCTTGTATGGCACTGGTGTTCCCACGCCAAACTCTTTCTACTACGAGAGCTTTCCTGATCGG GACCCCAAAATCTGCTTCGGCGATGGTGACGGCACGGTGAACCTGGAGAGCGTCCTGCAGTGCCAAGCCT GGCAGAGCCGCCAAGAGCACAGAGTATCATTGCAGGAGCTGCCGGGAAGCGAGCACATTGAGATGCTAGC CAATGCCACCACCTTGGCTTATCTGAAACGTGTGCTTCTGGAACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225901 representing NM_133792
Red=Cloning site Green=Tags(s) MDRHLCTCRETQLRSGLLLPLFLLMMLADLTLPAQRHPPVVLVPGDLGNQLEAKLDKPKVVHYLCSKKTD SYFTLWLNLELLLPVIIDCWIDNIRLVYNRTSRATQFPDGVDVRVPGFGETFSMEFLDPSKRNVGSYFYT MVESLVGWGYTRGEDVRGAPYDWRRAPNENGPYFLALREMIEEMYQMYGGPVVLVAHSMGNVYMLYFLQR QPQVWKDKYIHAFVSLGAPWGGVAKTLRVLASGDNNRIPVIGPLKIREQQRSAVSTSWLLPYNHTWSHEK VFVYTPTTNYTLRDYHRFFRDIGFEDGWFMRQDTEGLVEAMTPPGVELHCLYGTGVPTPNSFYYESFPDR DPKICFGDGDGTVNLESVLQCQAWQSRQEHRVSLQELPGSEHIEMLANATTLAYLKRVLLEP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133792 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133792.3 |
| RefSeq Size | 2721 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 192654 |
| UniProt ID | Q8VEB4 |
| Gene Summary | Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (PubMed:11790796, PubMed:16106046, PubMed:16880524, PubMed:19017977, PubMed:20410020). Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine (PubMed:16880524). Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen (PubMed:16880524, PubMed:19017977).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212446 | Pla2g15 (untagged) - Mouse phospholipase A2, group XV (Pla2g15), (10ug) |
CNY 3,656.00 |
|
| MR225901 | Pla2g15 (Myc-DDK-tagged) - Mouse phospholipase A2, group XV (Pla2g15) |
CNY 2,850.00 |
|
| MR225901L3 | Lenti ORF clone of Pla2g15 (Myc-DDK-tagged) - Mouse phospholipase A2, group XV (Pla2g15) |
CNY 4,750.00 |
|
| MR225901L4 | Lenti ORF clone of Pla2g15 (mGFP-tagged) - Mouse phospholipase A2, group XV (Pla2g15) |
CNY 4,750.00 |
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