Pla2g15 (NM_133792) Mouse Untagged Clone

CAT#: MC212446

Pla2g15 (untagged) - Mouse phospholipase A2, group XV (Pla2g15), (10ug)



  "NM_133792" in other vectors (4)

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms ACS; C87498; LLPL; Lpla2; Lypla3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212446 representing NM_133792
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCGCCATCTCTGCACCTGTCGCGAGACCCAGCTCCGGAGTGGCCTCCTGTTACCTCTGTTTCTAC
TAATGATGCTGGCAGACCTGACGCTCCCGGCCCAACGTCACCCCCCGGTGGTGCTGGTGCCTGGTGATTT
GGGTAACCAGTTGGAAGCAAAGCTGGATAAGCCAAAGGTTGTACACTACCTTTGCTCCAAGAAGACGGAC
AGCTACTTCACACTCTGGCTGAATCTGGAACTGCTTCTGCCTGTTATCATTGACTGCTGGATTGACAATA
TCAGGCTGGTTTACAACAGAACATCTCGGGCCACCCAGTTTCCCGATGGTGTGGACGTGCGTGTCCCTGG
CTTTGGGGAAACATTTTCTATGGAATTCCTAGACCCCAGCAAGAGGAATGTGGGTTCCTATTTCTACACT
ATGGTGGAGAGCCTTGTGGGCTGGGGCTACACACGGGGTGAAGACGTTCGAGGTGCTCCCTATGATTGGC
GGCGAGCCCCAAATGAAAACGGGCCCTACTTCTTGGCCCTGCGAGAGATGATCGAGGAGATGTACCAGAT
GTATGGGGGCCCCGTGGTGCTGGTCGCCCACAGCATGGGCAACGTGTACATGCTCTACTTTCTGCAGCGG
CAGCCACAAGTCTGGAAGGACAAATATATCCATGCCTTCGTCTCACTGGGGGCGCCCTGGGGGGGCGTGG
CCAAGACGCTGCGTGTCCTGGCCTCAGGAGACAACAATCGCATTCCCGTCATTGGGCCACTGAAGATCCG
GGAACAGCAGCGATCTGCCGTCTCTACCAGCTGGCTACTGCCATACAACCACACTTGGTCACATGAAAAG
GTATTTGTATACACACCCACGACTAACTACACGCTCCGGGACTATCACCGGTTCTTCCGGGACATCGGTT
TCGAAGATGGCTGGTTCATGCGGCAGGACACAGAAGGGCTGGTTGAAGCCATGACGCCACCCGGGGTGGA
GCTGCACTGCTTGTATGGCACTGGTGTTCCCACGCCAAACTCTTTCTACTACGAGAGCTTTCCTGATCGG
GACCCCAAAATCTGCTTCGGCGATGGTGACGGCACGGTGAACCTGGAGAGCGTCCTGCAGTGCCAAGCCT
GGCAGAGCCGCCAAGAGCACAGAGTATCATTGCAGGAGCTGCCGGGAAGCGAGCACATTGAGATGCTAGC
CAATGCCACCACCTTGGCTTATCTGAAACGTGTGCTTCTGGAACCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_133792
Insert Size 1239 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC019373, AAH19373
RefSeq Size 2721 bp
RefSeq ORF 1239 bp
Locus ID 192654
UniProt ID Q8VEB4
Gene Summary Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid (PubMed:11790796, PubMed:16106046, PubMed:16880524, PubMed:19017977, PubMed:20410020). Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine (PubMed:16880524). Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen (PubMed:16880524, PubMed:19017977).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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