Coq2 (NM_027978) Mouse Tagged ORF Clone

CAT#: MR205792

  • TrueORF®

Coq2 (Myc-DDK-tagged) - Mouse coenzyme Q2 homolog, prenyltransferase (yeast) (Coq2), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP



  "NM_027978" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,330.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2310002F18Rik; PHB:PPT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205792 representing NM_027978
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCTGGGGAGGCGCGGGGCTCGCGCGGGGCCTGCGGGCCGTTCGGTCCGCATGGCTGCGAGGTC
CGCGCGGGCTCCCATTGGCCCTGGTGCGCAGCGCAGGGGTTCCGGGCGCGCGGGATCGGCGAGCCCCGGC
CCCCGGGACGCAGCGGGGCCGAGCTCTCAGCCTGTCGGCCGCGGCCGTGGTGAACTCGGCGCCCCGCCCG
CTGCAGCCCTACCTGCGCCTCATGCGCCTGGACAAGCCCATAGGAACCTGGCTGCTGTATTTGCCATGCA
CTTGGAGCATTGGTCTGGCCGCTGACCCAGGTTGTTTTCCAGACTGGTACATGTTATCACTTTTTGGCAC
TGGAGCTATTCTGATGCGTGGAGCAGGCTGTACTATTAATGACATGTGGGACCGTGACTTCGATAAAAAG
GTTACAAGGACAGCAAACCGCCCAATCGCTGCTGGAGACATTTCGACCTTCCAGTCCTTCGTCTTCCTTG
GGGGACAGCTGACCTTGGCACTGGGCGTTCTCCTGTGTTTAAACTACTACAGTATAGCAATGGGAGCAGC
GTCCTTACTTCTTGTCGTCACCTACCCACTAGTGAAAAGAATCACGTTTTGGCCTCAGTTGGCTTTGGGA
TTGACTTTTAATTGGGGTGCGTTACTCGGATGGTCTGCTGTCAAGGGCTCCTGTGATCCGGCTGTATGCC
TGCCTCTGTATTTTTCTGGAGTTATGTGGACACTGATATATGATACTATCTATGCCCACCAGGACAAGAA
AGACGATGCTCTGATCGGCCTGAAGTCCACAGCACTGCTGTTCCAGGAGAACACAAGGCAGTGGCTTAGT
GGCTTTGGGGTCGCCATGGTGGCAGCGCTGAGCCTGGCGGGAGCCAACAATGGTCAGACTGTCCCCTACT
ACGCTGCTGTGGCTGCGGTGGGAGCCCACCTGGCTCACCAGATTTACACTGTAGACATCCACAGAGCCGA
GGACTGCTGGGACAAGTTCACCTCCAACCGCACAGTAGGAATGCTCCTGTTCTTAGGGATTGTCCTTGGA
AACCTGTGCAAAGAAAAGACGGAAGAAGCAAAAGATGCTGAAGCTGTTAGAGTGGGAAGTGAGCAGACGA
GC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205792 representing NM_027978
Red=Cloning site Green=Tags(s)

MLRWGGAGLARGLRAVRSAWLRGPRGLPLALVRSAGVPGARDRRAPAPGTQRGRALSLSAAAVVNSAPRP
LQPYLRLMRLDKPIGTWLLYLPCTWSIGLAADPGCFPDWYMLSLFGTGAILMRGAGCTINDMWDRDFDKK
VTRTANRPIAAGDISTFQSFVFLGGQLTLALGVLLCLNYYSIAMGAASLLLVVTYPLVKRITFWPQLALG
LTFNWGALLGWSAVKGSCDPAVCLPLYFSGVMWTLIYDTIYAHQDKKDDALIGLKSTALLFQENTRQWLS
GFGVAMVAALSLAGANNGQTVPYYAAVAAVGAHLAHQIYTVDIHRAEDCWDKFTSNRTVGMLLFLGIVLG
NLCKEKTEEAKDAEAVRVGSEQTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_027978
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_027978.2, NP_082254.2
RefSeq Size 1712 bp
RefSeq ORF 1125 bp
Locus ID 71883
UniProt ID Q66JT7
MW 41 kDa
Gene Summary Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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