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Abhd2 (NM_018811) Mouse Tagged ORF Clone
CAT＃: MR206769
- TrueORF®
Abhd2 (Myc-DDK-tagged) - Mouse abhydrolase domain containing 2 (Abhd2)
ORF Plasmid: tGFP
"NM_018811" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210009N18Rik; Labh-2; LABH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206769 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCCATGCTAGAGACCCCCGAGCTCCCCGCCGTGTTTGATGGGGTGAAGCTGGCTGCCGTAGCTG CCGTTCTCTACGTCATCGTGCGGTGTTTGAACCTGAAGAGCCCTACTGCCCCTCCTGACCTCTACTTCCA GGACTCCGGGCTCTCACGTTTCCTGCTCAAATCCTGTCCTCTTCTGACCAAAGAATACATTCCACCACTG ATCTGGGGGAAAAGCGGACATATCCAGACAGCCTTGTATGGGAAGATGGGGAGGGTGAGGTCACCACACC CTTACGGGCACCGCAAGTTCATCACCATGTCGGATGGTGCCACTTCTACCTTCGACCTCTTCGAGCCCCT GGCTGAGCACTGTGTTGGAGATGACATCACCATGGTCATCTGTCCTGGAATTGCCAACCACAGCGAGAAG CAGTATATCCGAACCTTCGTTGACTATGCCCAGAAAAATGGCTACCGGTGCGCAGTGCTAAACCACCTGG GAGCCCTCCCCAACATTGAGCTGACCTCCCCACGAATGTTCACCTATGGCTGCACGTGGGAATTTGGAGC CATGGTGAACTACATCAAGAGGACATATCCCCAGACCCAGCTGGTCGTCGTGGGCTTCAGCCTGGGTGGT AACATCGTGTGCAAATACTTGGGGGAGACGCAGGCAAACCAGGAAAAGGTCCTGTGCTGTGTCAGTGTGT GCCAGGGGTACAGCGCACTGAGGGCCCAGGAGACCTTCATGCAGTGGGACCAGTGCCGCAGGTTCTACAA CTTCCTCATGGCCGACAACATGAAGAAGATCATCCTGTCTCACAGACAAGCTCTCTTTGGAGACCACGTT AAGAAACCCCAGAGCCTGGAGGACACGGACTTGAGCCGGCTGTACACAGCAACATCCCTGATGCAGATTG ATGACAATGTGATGAGAAAGTTCCATGGCTATAATTCCCTGAAGGAATACTATGAGGAAGAGAGCTGCAT GAGGTACCTGCACAGGATATATGTGCCTCTCATGCTGGTTAATGCAGCTGACGACCCCTTGGTGCACGAA AGCCTTCTAACCATTCCAAAGTCTCTCTCAGAGAAACGGGAGAATGTCATGTTCGTGCTGCCTCTGCATG GGGGCCACCTGGGCTTCTTCGAGGGCTCCGTGCTGTTCCCCGAGCCGCTGACATGGATGGATAAGCTGGT AGTGGAGTATGCCAATGCCATTTGCCAATGGGAAAGGAATAAGTCCCAGTGCTCAGACACGGAGCAGATG GAGGCCGAGTTGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206769 protein sequence
Red=Cloning site Green=Tags(s) MNAMLETPELPAVFDGVKLAAVAAVLYVIVRCLNLKSPTAPPDLYFQDSGLSRFLLKSCPLLTKEYIPPL IWGKSGHIQTALYGKMGRVRSPHPYGHRKFITMSDGATSTFDLFEPLAEHCVGDDITMVICPGIANHSEK QYIRTFVDYAQKNGYRCAVLNHLGALPNIELTSPRMFTYGCTWEFGAMVNYIKRTYPQTQLVVVGFSLGG NIVCKYLGETQANQEKVLCCVSVCQGYSALRAQETFMQWDQCRRFYNFLMADNMKKIILSHRQALFGDHV KKPQSLEDTDLSRLYTATSLMQIDDNVMRKFHGYNSLKEYYEEESCMRYLHRIYVPLMLVNAADDPLVHE SLLTIPKSLSEKRENVMFVLPLHGGHLGFFEGSVLFPEPLTWMDKLVVEYANAICQWERNKSQCSDTEQM EAELE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018811 |
| ORF Size | 1278 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018811.6 |
| RefSeq Size | 2903 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 54608 |
| UniProt ID | Q9QXM0 |
| MW | 48.4 kDa |
| Gene Summary | Progesterone-dependent acylglycerol lipase that catalyzes hydrolysis of endocannabinoid arachidonoylglycerol (AG) from cell membrane. Acts as a progesterone receptor: progesterone-binding activates the acylglycerol lipase activity, mediating degradation of 1-arachidonoylglycerol (1AG) and 2-arachidonoylglycerol (2AG) to glycerol and arachidonic acid (AA). Also displays an ester hydrolase activity against acetyl ester, butanoate ester and hexadecanoate ester. Plays a key role in sperm capacitation in response to progesterone by mediating degradation of 2AG, an inhibitor of the sperm calcium channel CatSper, leading to calcium influx via CatSper and sperm activation (By similarity). Involved in acrosomal reaction (Probable). May also play a role in smooth muscle cells migration (PubMed:15721306).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202088 | Abhd2 (untagged) - Mouse abhydrolase domain containing 2 (Abhd2), (10ug) |
CNY 2,000.00 |
|
| MG206769 | Abhd2 (tGFP-tagged) - Mouse abhydrolase domain containing 2 (Abhd2) |
CNY 3,140.00 |
|
| MR206769L3 | Lenti ORF clone of Abhd2 (Myc-DDK-tagged) - Mouse abhydrolase domain containing 2 (Abhd2) |
CNY 4,750.00 |
|
| MR206769L4 | Lenti ORF clone of Abhd2 (mGFP-tagged) - Mouse abhydrolase domain containing 2 (Abhd2) |
CNY 4,750.00 |
