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Trpv6 (NM_022413) Mouse Tagged ORF Clone
CAT＃: MR210315
- TrueORF®
Trpv6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6)
ORF Plasmid: tGFP
"NM_022413" in other vectors (4)
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CNY 8,000.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cac; CAT; CaT1; Ecac2; Otrpc3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210315 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTGGTCCCTGCCCAAGGAGAAGGGGTTAATACTCTGCCTATGGAACAAGTTCTGCAGATGGTTCC ACAGACAAGAGTCCTGGGCTCAGAGCCGAGACGAGCAGAACCTGCTGCAGCAGAAGAGGATCTGGGAATC ACCTCTTCTTCTAGCTGCCAAAGAAAACGATGTCCAGGCTCTGAGCAAGCTGCTCAAGTTTGAAGGATGT GAGGTGCACCAGAGAGGAGCCATGGGGGAAACTGCACTTCACATAGCAGCCCTCTATGATAACCTGGAGG CTGCAATGGTGCTAATGGAGGCTGCCCCAGAACTAGTCTTTGAGCCCATGACTTCGGAGCTATATGAAGG TCAGACTGCACTACACATTGCAGTAATAAACCAGAATGTAAACTTGGTCCGTGCTCTGCTTGCCCGAGGG GCCAGTGTCTCTGCCAGAGCTACAGGCTCTGTCTTCCACTACAGGCCTCACAACCTCATTTACTATGGAG AGCACCCTTTGTCCTTTGCTGCCTGTGTGGGTAGTGAGGAGATTGTTAGACTGCTCATTGAGCATGGAGC TGACATCCGTGCCCAGGACTCTCTGGGAAATACAGTACTACACATACTCATCCTGCAGCCCAACAAAACC TTTGCCTGCCAGATGTACAACTTGCTACTGTCCTATGATGGGGGAGACCACCTGAAGTCCCTGGAACTTG TGCCAAATAACCAGGGACTCACCCCCTTCAAGTTGGCTGGGGTGGAAGGCAACATTGTGATGTTCCAACA CCTGATGCAGAAGCGGAAACACATCCAGTGGACATATGGGCCATTGACCTCCACACTGTATGACCTCACT GAGATTGACTCCTCAGGAGATGATCAATCCCTACTGGAACTTATTGTTACTACCAAGAAACGGGAGGCTC GCCAGATCCTGGACCAGACACCTGTAAAGGAACTGGTGAGCCTCAAGTGGAAGAGGTATGGGCGGCCCTA CTTCTGCGTGCTGGGTGCCATCTATGTGCTGTACATCATCTGCTTTACCATGTGCTGTGTCTACCGCCCG CTCAAGCCCAGGATCACTAACCGCACCAACCCCCGGGACAACACTCTGATGCAACAGAAGCTCCTTCAGG AGGCCTATGTGACCCCTAAGGATGACCTCCGGTTGGTGGGGGAACTCGTGAGCATCGTTGGGGCTGTGAT CATCCTGCTGGTGGAGATTCCAGACATCTTCAGGTTGGGGGTCACTCGATTCTTTGGACAGACCATTCTC GGGGGGCCATTCCATGTCATCATTATCACTTATGCCTTCATGGTGCTGGTGACCATGGTGATGCGACTCA CCAATGTAGATGGAGAAGTGGTGCCCATGTCCTTTGCTCTGGTGTTGGGCTGGTGCAATGTCATGTACTT TGCCAGAGGATTCCAGATGCTGGGTCCCTTCACCATCATGATTCAGAAGATGATTTTTGGTGACTTGATG CGATTCTGCTGGCTGATGGCTGTGGTAATTCTGGGATTTGCTTCAGCCTTCTATATCATTTTCCAGACAG AGGATCCCGATGAGCTGGGTCATTTCTATGACTACCCCATGGCACTGTTCAGCACCTTTGAACTCTTCCT CACCATCATCGATGGCCCTGCCAACTATGACGTGGATCTGCCCTTCATGTACAGCGTTACCTACGCTGCC TTTGCCATCATCGCCACACTGCTCATGCTTAACCTCCTCATTGCCATGATGGGTGACACTCACTGGAGAG TTGCCCATGAGCGAGATGAGCTCTGGAGAGCACAGGTTGTGGCCACCACTGTGATGTTAGAGCGGAAGCT ACCTCGTTGCCTGTGGCCTCGCTCTGGGATATGTGGGCGAGAGTATGGTCTTGGCGACCGCTGGTTCTTG AGGGTGGAAGACAGGCAAGATCTCAACAGACAACGCATCCGCCGCTATGCACAGGCCTTCCAGCAACAAG ATGGCCTCTACTCTGAGGACTTGGAAAAAGATTCAGGAGAAAAACTGGAGACAGCACGTCCCTTTGGTGC CTATCTGTCCTTTCCTACACCCTCAGTATCTCGAAGTACCTCCCGAAGCAGCACCAATTGGGAAAGGCTT CGACAAGGGGCCCTGAGGAAGGACCTGCGGGGGATAATCAACCGGGGCCTAGAAGATGGGGAGGGCTGGG AGTACCAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210315 protein sequence
Red=Cloning site Green=Tags(s) MGWSLPKEKGLILCLWNKFCRWFHRQESWAQSRDEQNLLQQKRIWESPLLLAAKENDVQALSKLLKFEGC EVHQRGAMGETALHIAALYDNLEAAMVLMEAAPELVFEPMTSELYEGQTALHIAVINQNVNLVRALLARG ASVSARATGSVFHYRPHNLIYYGEHPLSFAACVGSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKT FACQMYNLLLSYDGGDHLKSLELVPNNQGLTPFKLAGVEGNIVMFQHLMQKRKHIQWTYGPLTSTLYDLT EIDSSGDDQSLLELIVTTKKREARQILDQTPVKELVSLKWKRYGRPYFCVLGAIYVLYIICFTMCCVYRP LKPRITNRTNPRDNTLMQQKLLQEAYVTPKDDLRLVGELVSIVGAVIILLVEIPDIFRLGVTRFFGQTIL GGPFHVIIITYAFMVLVTMVMRLTNVDGEVVPMSFALVLGWCNVMYFARGFQMLGPFTIMIQKMIFGDLM RFCWLMAVVILGFASAFYIIFQTEDPDELGHFYDYPMALFSTFELFLTIIDGPANYDVDLPFMYSVTYAA FAIIATLLMLNLLIAMMGDTHWRVAHERDELWRAQVVATTVMLERKLPRCLWPRSGICGREYGLGDRWFL RVEDRQDLNRQRIRRYAQAFQQQDGLYSEDLEKDSGEKLETARPFGAYLSFPTPSVSRSTSRSSTNWERL RQGALRKDLRGIINRGLEDGEGWEYQI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022413 |
| ORF Size | 2184 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022413.3 |
| RefSeq Size | 2946 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 64177 |
| UniProt ID | Q91WD2 |
| MW | 83.2 kDa |
| Gene Summary | Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine (PubMed:12765696, PubMed:12601087, PubMed:12574114, PubMed:14736889, PubMed:15123711, PubMed:17129178). Important for normal Ca(2+) ion homeostasis in the body, including bone and skin (PubMed:17129178, PubMed:22878123). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation (PubMed:15123711). In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner (PubMed:12601087). Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (PubMed:12574114).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221065 | Trpv6 (untagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6), (10ug) |
CNY 9,310.00 |
|
| MG210315 | Trpv6 (tGFP-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6) |
CNY 9,600.00 |
|
| MR210315L3 | Lenti ORF clone of Trpv6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6) |
CNY 8,270.00 |
|
| MR210315L4 | Lenti ORF clone of Trpv6 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily V, member 6 (Trpv6) |
CNY 10,400.00 |
