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Ube4b (NM_022022) Mouse Tagged ORF Clone
CAT＃: MR211747
- TrueORF®
Ube4b (Myc-DDK-tagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (Ube4b)
ORF Plasmid: tGFP
"NM_022022" in other vectors (4)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; mKIAA0684; UFD2; UFD2a; Ufd2p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211747 representing NM_022022
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTGAGCGCTGACGAGATTCGACGGAGGCGCCTGGCACGACTTGCTGGTGGACAGACCTCCC AGCCGACCACCCCGCTTACATCTCCCCAGAGGGAGAACCCTCCGGGACCTCCAATAGCTGCATCAGCCCC AGGCCCCTCCCAGAGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCCATAGGTGCAGCA GGAGTTGCCCATCGGAGCCAGAGCAGCGAAGGCGTCAGTTCTCTCAGCAGCTCACCTTCCAACAGCCTTG AGACACAGTCTCAGTCCCTCTCCCGTTCCCAGAGCATGGATATTGACGGTGTCTCCTGTGAGAAAAGCAT GTCCCAGGTGGATGTGGATTCGGGAATTGAGAATATGGAGGTGGACGAGAACGACCGAAGAGAAAAACGG AGCCTGAGTGACAAGGAGCCTTCTTCAGGTCCTGAGGTGTCTGAAGAGCAAGCCTTACAGCTGGTCTGTA AGATCTTCCGTGTCTCCTGGAAGGACCGCGACAGAGATGTCATCTTCCTTTCTTCTCTCTCTGCGCAGTT TAAACAGAACCCAAAAGAAGTGTTCTCTGATTTTAAGGATCTGATTGGCCAGATTTTAATGGAAGTACTG ATGATGTCAACTCAGACACGAGATGAAAATCCATTTGCCAGTCTGACAGCCACATCCCAGCCCATCGCCA CGGCAGCTCGGTCACCAGACCGCAATCTCATGCTGAACACTGGCTCCAGTTCAGGAACAAGCCCCATGTT CTGCAACATGGGCTCCTTCAGCACCAGCTCGTTGTCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAACTGG GATTCGTACAGTGACCATTTCACCATTGAAACCTGCAAAGAGACGGACATGCTGAACTACCTCATCGAGT GTTTTGACCGAGTTGGAATAGAGGAAAAAAAAGCACCAAAGATGTGCAGCCAGCCAGCAGTCAGCCAGCT TCTGAGCAACATCCGCTCGCAGTGCATCTCCCACACCGCACTAGTGCTACAAGGTTCCCTCACACAGCCC AGGTCCTTGCAACAGCCATCCTTCCTCGTGCCGTACATGCTCTGCAGGAACCTCCCGTACGGCTTCATTC AAGAGCTGGTGAGGACCACTCACCAGGATGAAGAGGTGTTCAAGCAGATCTTTATCCCCATTTTACAAGG CCTGGCGCTGGCTGCCAAAGAGTGCTCCTTGGAAAGCGACTACTTTAAGTACCCCCTCATGGCACTGGGT GAACTCTGTGAAACCAAGTTTGGGAAGACACACCCTATGTGCAATTTGGTCGCTTCTTTACCCCTGTGGT TGCCGAAGTCCTTAAGCCCTGGTTCTGGGCGGGAGCTGCAGAGACTCTCCTACTTAGGGGCCTTCTTTAG CTTCTCAGTCTTCGCAGAGGACGATGCAAAGGTGGTTGAGAAATACTTCTCAGGGCCTGCCATTACCCTG GAGAACACACGCGTGGTCAGCCAGTCACTACAGCATTACCTGGAGCTGGGACGGCAAGAACTTTTCAAGA TTCTACATAGTATTTTGTTAAATGGCGAAACTCGTGAAGCGGCTCTCAGCTACATGGCGGCCCTTGTCAA TGCCAACATGAAGAAAGCCCAGATGCAGGCAGATGATCGGTTGGTGTCTACAGACGGGTTTATGCTGAAT CTCCTTTGGGTCCTGCAGCAGCTAAGTACAAAAATCAAGTTAGAAACAGTTGACCCCACTTACATATTCC ACCCAAGATGTCGGATCACTCTCCCGAATGATGAGACACGGATAAATGCAACGATGGAGGATGTGAATGA GCGGCTGACAGAGCTCTATGGTGATCAGCCTCCATTTTCTGAGCCAAAATTCCCTACGGAATGTTTCTTT CTCACCCTGCATGCTCATCACCTCTCTATTCTGCCTAGTTGTCGTCGCTACATTCGCCGACTTCGAGCCA TCCGCGAGCTCAATAGAACCGTGGAAGATTTGAAAAATAATGAAAGCCAGTGGAAAGATTCCCCACTGGC CACCAGACACCGTGAAATGCTGAAGCGCTGTAAAACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGT GCTGACGCTGGCTTACTTGACGAGAGCTTCCTAAGAAGATGCCTGAATTTTTATGGCCTTCTCATTCAGC TGATGCTGCGCATCCTGGACCCTGCATATCCTGACGTAACACTGCCTTTGAATTCAGAAGTCCCCAAGGT ATTTGCAGCATTGCCTGAGTTTTATGTCGAAGATGTTGCTGAATTTTTATTTTTTATTGTACAATACTCT CCTCAGGTGCTTTATGAGCCCTGCACTCAGGACATTGTCATGTTCCTCGTTGTGATGCTGTGCAACCAGA ACTACATCCGAAACCCTTACCTAGTGGCCAAGCTGGTGGAGGTCATGTTCATGACCAACCCTTCTGTCCA GCCGAGAACTCAGAAGTTCTTTGAGATGATTGAGAACCATCCTCTCTCTACCAAACTGCTGGTGCCGTCC CTCATGAAGTTCTATACAGATGTCGAGCATACTGGAGCCACCAGCGAGTTCTATGACAAGTTCACAATTC GCTATCACATTAGCACTATTTTTAAAAGCCTTTGGCAAAACATAGCTCACCATGGCACCTTCATGGAGGA GTTCAATTCTGGAAAGCAGTTCGTTCGGTATATCAATATGCTAATAAATGACACGACCTTTTTACTGGAC GAAAGTCTGGAGTCTCTGAAGCGGATCCATGAAGTGCAAGAAGAGATGAAGAACAAAGAACAGTGGGACC AGCTGCCTCGGGATCAGCAGCAGGCCCGGCAGTCTCAGCTTGCTCAGGACGAGCGTGTTTCCCGTTCTTA TCTTGCCCTGGCAACTGAAACCGTGGACATGTTCCATCTCCTCACCAAGCAAGTCCAGAAGCCGTTCCTG AGGCCAGAGCTTGGTCCCCGATTAGCAGCCATGCTGAACTTTAACCTGCAGCAGCTCTGCGGACCCAAGT GCCGGGACCTGAAAGTTGAAAACCCAGAGAAGTATGGTTTTGAGCCAAAGAAGCTGTTGGACCAACTGAC AGATATTTATTTACAATTGGACTGTGCTCGCTTTGCTAAAGCCATCGCCGATGACCAGCGATCCTACAGC AAGGAACTGTTTGAAGAAGTCATTTCAAAGATGCGGAAGGCGGGAATCAAATCCACCATCGCAATAGAGA AGTTTAAACTTCTTGCAGAGAAAGTGGAGGAAATCGTGGCAAAGAATGCGCGGGCAGAAATAGACTACAG CGATGCCCCGGACGAGTTCAGAGACCCTCTGATGGACACCCTGATGACCGATCCCGTGAGACTGCCCTCT GGCACCGTCATGGACCGTTCTATCATCCTGCGGCATCTGCTCAACTCCCCCACCGACCCCTTCAACCGCC AGATGCTGACTGAGAGCATGCTGGAGCCAGTGCCAGAGCTAAAGGAGCAGATTCAGGCCTGGATGAGAGA GAAACAGAGCAGTGACCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211747 representing NM_022022
Red=Cloning site Green=Tags(s) MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAA GVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKR SLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVL MMSTQTRDENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCNMGSFSTSSLSSLGASGGASNW DSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQP RSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLESDYFKYPLMALG ELCETKFGKTHPMCNLVASLPLWLPKSLSPGSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITL ENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAALVNANMKKAQMQADDRLVSTDGFMLN LLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNERLTELYGDQPPFSEPKFPTECFF LTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKAC ADAGLLDESFLRRCLNFYGLLIQLMLRILDPAYPDVTLPLNSEVPKVFAALPEFYVEDVAEFLFFIVQYS PQVLYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPS LMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLD ESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLTKQVQKPFL RPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYS KELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPS GTVMDRSIILRHLLNSPTDPFNRQMLTESMLEPVPELKEQIQAWMREKQSSDH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022022 |
| ORF Size | 3519 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022022.3, NP_071305.2 |
| RefSeq Size | 5535 bp |
| RefSeq ORF | 3522 bp |
| Locus ID | 63958 |
| UniProt ID | Q9ES00 |
| MW | 133.3 kDa |
| Gene Summary | Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (PubMed:11435423). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202594 | Ube4b (untagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (Ube4b), (10ug) |
CNY 8,176.00 |
|
| MG211747 | Ube4b (tGFP-tagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S |
CNY 12,830.00 |
|
| MR211747L3 | Lenti ORF clone of Ube4b (Myc-DDK-tagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (Ube4b) |
CNY 13,590.00 |
|
| MR211747L4 | Lenti ORF clone of Ube4b (mGFP-tagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (Ube4b) |
CNY 13,590.00 |
