Ube4b (NM_022022) Mouse Tagged Lenti ORF Clone

CAT#: MR211747L4

  • LentiORF®

Lenti ORF clone of Ube4b (mGFP-tagged) - Mouse ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) (Ube4b)



  "NM_022022" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 13,590.00


货期*
10周

规格
    • 10 ug

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00


Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

CNY 2,950.00


Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00


Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag mGFP
Synonyms 4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; mKIAA0684; UFD2; UFD2a; Ufd2p
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(MR211747).
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene     
ACCN NM_022022
ORF Size 3519 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022022.3, NP_071305.2
RefSeq Size 5535 bp
RefSeq ORF 3522 bp
Locus ID 63958
UniProt ID Q9ES00
Gene Summary Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (PubMed:11435423). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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