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Map2k7 (NM_011944) Mouse Tagged ORF Clone
CAT＃: MR216601
- TrueORF®
Map2k7 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 7 (Map2k7), transcript variant 2
ORF Plasmid: tGFP
"NM_011944" in other vectors (4)
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CNY 4,180.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5930412N11Rik; JNKK 2; Jnkk2; MAPKK 7; Mapkk7; MEK 7; Mek7; Mkk7; Prkmk7; sek2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216601 representing NM_011944
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTCCTCCCTGGAGCAGAAGCTGTCCCGCCTGGAAGCCAAGCTGAAGCAGGAGAACCGTGAGG CCCGCAGGAGGATCGACCTCAACTTGGATATCAGCCCACAGCGGCCCAGGCCCACCCTGCAACTCCCACT GGCCAACGATGGGGGCAGCCGCTCACCATCCTCAGAGAGCTCCCCACAGCACCCTACACCCCCCACCCGG CCCCGCCACATGCTGGGGCTCCCATCAACCTTGTTCACACCGCGCAGTATGGAGAGCATCGAGATTGACC AGAAGCTGCAGGAGATCATGAAGCAGACAGGGTACCTGACTATCGGGGGCCAGCGTTATCAGGCAGAAAT CAATGACTTGGAGAACTTGGGTGAGATGGGCAGTGGTACCTGTGGTCAGGTGTGGAAGATGCGGTTCCGG AAGACAGGCCACATCATTGCTGTTAAGCAAATGCGGCGCTCTGGGAACAAGGAAGAGAATAAGCGCATTT TGATGGACCTGGATGTAGTACTCAAGAGCCATGACTGCCCTTACATCGTTCAGTGCTTTGGCACCTTCAT CACCAACACAGACGTCTTTATTGCCATGGAGCTCATGGGCACATGTGCAGAGAAGCTGAAGAAACGAATG CAGGGCCCCATTCCAGAGCGAATCCTGGGCAAGATGACTGTGGCGATTGTGAAAGCACTGTACTATCTGA AGGAGAAGCATGGCGTCATCCATCGCGATGTCAAACCCTCCAACATCCTGCTAGATGAGCGGGGCCAGAT CAAGCTCTGTGACTTTGGCATCAGTGGCCGCCTTGTTGACTCCAAAGCCAAAACACGGAGTGCTGGCTGT GCTGCCTATATGGCTCCCGAGCGCATCGACCCTCCAGATCCCACCAAGCCTGACTATGACATCCGAGCTG ATGTGTGGAGCCTGGGCATCTCACTGGTGGAGCTGGCAACAGGACAGTTCCCCTATAAGAACTGCAAGAC GGACTTTGAGGTCCTCACCAAAGTCCTACAGGAAGAGCCCCCACTCCTGCCTGGTCACATGGGCTTCTCA GGGGACTTCCAGTCATTTGTCAAAGACTGCCTTACTAAAGATCACAGGAAGAGACCAAAGTATAATAAGC TACTTGAACACAGCTTCATCAAGCACTATGAGATACTCGAGGTGGATGTCGCGTCCTGGTTTAAGGATGT CATGGCGAAGACCGAGTCCCCAAGGACTAGTGGAGTCCTGAGTCAGCACCATCTGCCCTTCTTCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216601 representing NM_011944
Red=Cloning site Green=Tags(s) MAASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPRPTLQLPLANDGGSRSPSSESSPQHPTPPTR PRHMLGLPSTLFTPRSMESIEIDQKLQEIMKQTGYLTIGGQRYQAEINDLENLGEMGSGTCGQVWKMRFR KTGHIIAVKQMRRSGNKEENKRILMDLDVVLKSHDCPYIVQCFGTFITNTDVFIAMELMGTCAEKLKKRM QGPIPERILGKMTVAIVKALYYLKEKHGVIHRDVKPSNILLDERGQIKLCDFGISGRLVDSKAKTRSAGC AAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEEPPLLPGHMGFS GDFQSFVKDCLTKDHRKRPKYNKLLEHSFIKHYEILEVDVASWFKDVMAKTESPRTSGVLSQHHLPFFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011944 |
| ORF Size | 1257 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011944.3, NP_036074.2 |
| RefSeq Size | 3515 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 26400 |
| UniProt ID | Q8CE90 |
| MW | 48 kDa |
| Gene Summary | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209719 | Map2k7 (untagged) - Mouse mitogen-activated protein kinase kinase 7 (Map2k7), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG216601 | Map2k7 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase 7 (Map2k7) transcript variant 2, (10ug) |
CNY 4,560.00 |
|
| MR216601L3 | Lenti ORF clone of Map2k7 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 7 (Map2k7), transcript variant 2 |
CNY 6,080.00 |
|
| MR216601L4 | Lenti ORF clone of Map2k7 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 7 (Map2k7), transcript variant 2 |
CNY 6,080.00 |
