Ptcra (NM_011195) Mouse Tagged ORF Clone
CAT#: MR217468
- TrueORF®
Ptcra (Myc-DDK-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra)
ORF Plasmid: tGFP
"NM_011195" in other vectors (4)
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CNY 1,416.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | gp33; pT-alpha; pT-alpha-TCR; pTa; pTalpha; pT[a] |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217468 representing NM_011195
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCTCCACGAGTGGGCCATGGCTAGGACATGGCTGCTGCTGCTTCTGGGCGTCAGGTGTCAGGCTC TACCATCAGGCATCGCTGGCACCCCCTTTCCGTCTCTGGCTCCACCCATCACACTGCTGGTAGATGGAAG GCAGCACATGCTGGTGGTTTGCCTGGTCCTCGATGCGGCACCCCCTGGCCTTGACAACCCTGTCTGGTTC TCAGCTGGCAATGGCAGTGCCCTAGACGCCTTCACCTATGGGCCCTCTCTGGCACCGGACGGCACCTGGA CTAGCCTGGCCCAGCTCTCCTTGCCTTCTGAAGAGCTGGAAGCCTGGGAGCCCTTGGTCTGTCACACCAG GCCTGGGGCTGGGGGACAGAACCGGAGCACACACCCCCTCCAGCTGTCAGGGGAATCTTCGACAGCCAGG AGCTGCTTTCCGGAGCCTCTCGGGGGGACACAGCGGCAGGTACTGTGGCTGAGCCTACTGCGCCTGCTCC TCTTCAAACTGCTTCTGCTCGATGTGCTCCTGACCTGCAGCCACCTCCGCCTCCATGTGCTGGCGGGCCA GCACCTCCAGCCACCACCCTCACGCAAGTCCCTGCCTCCCACCCACAGAATTTGGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217468 representing NM_011195
Red=Cloning site Green=Tags(s) MLLHEWAMARTWLLLLLGVRCQALPSGIAGTPFPSLAPPITLLVDGRQHMLVVCLVLDAAPPGLDNPVWF SAGNGSALDAFTYGPSLAPDGTWTSLAQLSLPSEELEAWEPLVCHTRPGAGGQNRSTHPLQLSGESSTAR SCFPEPLGGTQRQVLWLSLLRLLLFKLLLLDVLLTCSHLRLHVLAGQHLQPPPSRKSLPPTHRIWT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011195 |
ORF Size | 618 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011195.2, NP_035325.1 |
RefSeq Size | 1283 bp |
RefSeq ORF | 621 bp |
Locus ID | 19208 |
UniProt ID | P0C6B2 |
MW | 22.8 kDa |
Gene Summary | The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. Isoform 1 acts to retain most TCRB intracellularly, while isoform 2 permits higher levels of cell surface TCRB expression and facilitates signaling from the CD3-TCRB complex.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209251 | Ptcra (untagged) - Mouse pre T-cell antigen receptor alpha (Ptcra), (10ug) |
CNY 3,990.00 |
|
MG217468 | Ptcra (tGFP-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra), (10ug) |
CNY 2,850.00 |
|
MR217468L3 | Lenti ORF clone of Ptcra (Myc-DDK-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra) |
CNY 4,750.00 |
|
MR217468L4 | Lenti ORF clone of Ptcra (mGFP-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra) |
CNY 4,750.00 |