Rab11fip3 (NM_001162869) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cart1; D030060O14Rik; mKIAA0665; Rab11-FIP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217641 representing NM_001162869, codon optimized. Due to the complexity of NM_001162869, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTTGTCAGCCTACAAGCCTGTCAGATCATGATCAACCGGCCAGTGGGCCGCAGCGGGGAGTGA TGGGGTTGGTGGGACCCGACGCTCCCCGGGGTTGGTCAGAGGAACCCGAAGAACACGCTCAACTGCAGCG CTGGCCAGAGGGTCCAAACGCTCCTATCTGCTGGCCTGAGGAGGTGGAGGAACCTCATGCCCCCTCCCGA TGGGCGAAGGAACCCAACGCGCCAAGATGTTCCTCCCAGGAGCCAGATGAGTCATGTCACTTGGCTGAAG AGTTGGAGGAGAGCGACTCTCCACGCTGCTGGCCTCAAGAGCCAGATACACCTTGTCACCTTGCCAAGGA ACTCGAAGAGCCTGATGCACCTCGCTGTCTCCCACAGGAGCCAGACACACCATGCTATCTCGCAAAAGAA CTGGAGGAGCCAAATATCCCACGGTGTTGGCCCCAGGAGCCTGATGTCCCTTGTCACCTTGCCAAGGAGC TCGAGGAACCTGATGCCCCCAGATGTTGGCCCCAGGAACCAGATGCCTTCTGTCATCTCCTTAAGGAAGT GGAGGAGCCCGACGCCCTGAGGTGTTGGCTGCAGGGTCCTGACGCGCCTTGTCATCTTGCCAAAGAACTG GAGGACTTGGACTCCCCAAGATGCTGGCCTCAAGAGCCTGATGAATCATGCCACCTTGCGAAAGAACTGG AAGAACCAGATGCCCCCTGCCATTTGGCCAAAGAGCTGGAAGAGCCCGATGCGCCTCGATGTTGGCCACA AGAGCCAGATGTACCTTGTCTTCTTGCAAAGAAATGGGAGGAGAGTGATGCCCCCTGTCTCTTGACAGAA GAGCTCGAAGAGCCAGATGCTCTTCACTGCTGGCCACAAGAATCCGAGGCCCCTTGTCTCCTTGCAAAGG AGTTGGAAGAACCTGACGCTAGCCACTCCTGTCCCCAGGAGGCTGATACGGGCTGTCTGTCTGCAAAAGA GCCAGAGGAGCCTGACGTCTCCCACCTGTGGCAGGGGGTGCCTGATGCCCCATGTTTGCTGGTTAAGGAG CCAGAAGAAGCGGATGCCTTGCACTGTTGCTGGCCGGAGGAGAGCGAGGAACCAGACGCTCTCAATCCTC CTTGTTTTTGGGCTAATGAGCCCGATGAGCCTGATCCCTCCAGATGCTGGAGCGAGGAGCCTCAGGTGCT CTGTCTTTGGCCTGAGGAGCAGAATACCAAAAGATGTTGGCAGGAAGAGCCCGATGCTCCGTGCTTCTGG CCAGAAGATAGAGAAGAGCCTATCGTATCTTGCCTGCAATTTAAGGAGCCTGAGAAACCTAAGGTTCGGA GCAGTTGGCCAGAAGAACTTGAAGACTGCTGCCCAACACGAGGATTGCCTCTGGAGCCTCTGTTGGCCGA CGGAGAGCTGCTCCAGGCATGTCCGGGGCCACCATCCGATCCAGGTCCCGCTCTCTCTTTGCCTTCTGAA CCCGGAACAGCCCAGGAGGAGGGTGCTCGGCTTCGCGCTGTCTTCGACGCTTTGGACCGAGACGGGGACG GATTCGTCCGCATCGAGGACTTCATCCAGTTCGCAACCGTGTACGGCGCCGAACAAGTGAAAGATCTTAC CCAGTACCTCGATCCGAGTGGCCTGGGAGTCATTTCATTTGAGGATTTCTACCAAGGCATCGTGGCAATC CGCAACGGGGACCCTGACGGGCAACTGTATTCAGTGGAACCGGTGCAGGACGAGGAGACTCCTGCTTGCG CAGACGAGTTTGATGACTTTGTGACATATGAGGCCAACGAAGTTACCGACTCTGCTTATATGGGGTCAGA GAGCACATACTCCGAGTGCGAAACCTTCACCGACGAGGACACAAGCACCCTCGTCCATCCTGAGTTGCAA CCTGAGGGGGATGTGGACAGCGCTGGCGGTTCTGGCGTCCCAAGCGAGTGTCTGGACACGATGGAGGAGC CCGACCATGGCGCTCTTTTGCTTCTCCCGGGTAGGAGCAGGCCCCATTCTCAGGCCGTGGTGATGGTCAT TGGCTCAGAGGAACATTTCGAAGACTATGGAGAAGGAAATGAGGCCGAACTCAGTCCAGAAACCTTGTGT GATGGCGACGGAGAGGACCCGGCCTTCCTCACCCCAAGTTCTAACCCACTGGCTTCAAAGCTCTGTGACG TTCTTACTGATGAGGCCTTCGAGTTCTACTGTTCCCAGTGTCACAAGCAGATTAACAGGCTTGAAGACCT CTCCGCTCGCCTGACCGACCTGGAGATGAACAGCCCAGCTAAACGCCTGTCCAGCAGAAAAGTGGCCAGA TACCTCCACCAGAGCGGAACCCTGACAATGGAGGCTCTTGAGGACCCACCACCTGAACCAGTAGAGTGCC CAGAGGAAGACATCGCTGATAAGGTGATCTTTCTGGAGCGCCGCGTGAGTGAGCTGGAAAAGGATTCCGC GGCCGCTGGTGAGCAACATGGCAGACTGCGACAGGAGAATTTGCAACTGGTCCACCGGGCGAATGCACTC GAAGAGCAGCTCAAGGAGCAGGAGTTCCGGGCCCAGGAAAAGGTGCTGGAAGAAACAAGGAAGCAGAAGG AACTGCTCTGTAAGATGGAACGCGAGAAATCCATAGAAATCGAGAATCTGCAAGCACGCCTGCAGCAGCT GGACGAGGAGAACTCTGAGCTCCGATCCTGCACTCCATGCCTGAAGGCCAACATCGAACGCCTTGAGGAG GAGAAGCAGAAGATGCTTGATGAAATTGAGGAGCTGACACAGAGGCTGTCCGAGGAACAGGAGAACAAAA GAAAAATGGGTGATCGGCTGTCACACGAGCGCCATCAGTTCCAACGGGACAAAGAGGCCACTCAGGAGCT TATTGAGGACCTGCGGAAGCAGCTCGAGCACTTGCAGCTGCTGCGGCTGGAGGTGGAGCAGAGAAGAGGC AGAAGCAGTTCACTGGGACTCCAGGAGTATAATTCTCGGGCACGGGAGAGTGAGCTCGAACAGGAGGTAA GGAGGCTGAAGCAGGACAACAGAAATCTGAAGGAGCAGAACGATGAACTTAACGGCCAGATCATCACCCT CAGTATCCAGGGCGCGAAGTCCCTCTTCTCCACCTCTTTTTCCGAGAGCTTGGCTGCTGAAATCTCCAGC GTCAGCAGAGACGAATTGATGGAAGCCATCCAGAAGCAGGAGGAAATTAATTTCCGCCTTCAGGATTATA TCGATAGGATCATCGTGGCAATTCTGGAGACTAATCCCTCTATATTGGAAGTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217641 representing NM_001162869 Red=Cloning site Green=Tags(s) MELCQPTSLSDHDQPASGPQRGVMGLVGPDAPRGWSEEPEEHAQLQRWPEGPNAPICWPEEVEEPHAPSR WAKEPNAPRCSSQEPDESCHLAEELEESDSPRCWPQEPDTPCHLAKELEEPDAPRCLPQEPDTPCYLAKE LEEPNIPRCWPQEPDVPCHLAKELEEPDAPRCWPQEPDAFCHLLKEVEEPDALRCWLQGPDAPCHLAKEL EDLDSPRCWPQEPDESCHLAKELEEPDAPCHLAKELEEPDAPRCWPQEPDVPCLLAKKWEESDAPCLLTE ELEEPDALHCWPQESEAPCLLAKELEEPDASHSCPQEADTGCLSAKEPEEPDVSHLWQGVPDAPCLLVKE PEEADALHCCWPEESEEPDALNPPCFWANEPDEPDPSRCWSEEPQVLCLWPEEQNTKRCWQEEPDAPCFW PEDREEPIVSCLQFKEPEKPKVRSSWPEELEDCCPTRGLPLEPLLADGELLQACPGPPSDPGPALSLPSE PGTAQEEGARLRAVFDALDRDGDGFVRIEDFIQFATVYGAEQVKDLTQYLDPSGLGVISFEDFYQGIVAI RNGDPDGQLYSVEPVQDEETPACADEFDDFVTYEANEVTDSAYMGSESTYSECETFTDEDTSTLVHPELQ PEGDVDSAGGSGVPSECLDTMEEPDHGALLLLPGRSRPHSQAVVMVIGSEEHFEDYGEGNEAELSPETLC DGDGEDPAFLTPSSNPLASKLCDVLTDEAFEFYCSQCHKQINRLEDLSARLTDLEMNSPAKRLSSRKVAR YLHQSGTLTMEALEDPPPEPVECPEEDIADKVIFLERRVSELEKDSAAAGEQHGRLRQENLQLVHRANAL EEQLKEQEFRAQEKVLEETRKQKELLCKMEREKSIEIENLQARLQQLDEENSELRSCTPCLKANIERLEE EKQKMLDEIEELTQRLSEEQENKRKMGDRLSHERHQFQRDKEATQELIEDLRKQLEHLQLLRLEVEQRRG RSSSLGLQEYNSRARESELEQEVRRLKQDNRNLKEQNDELNGQIITLSIQGAKSLFSTSFSESLAAEISS VSRDELMEAIQKQEEINFRLQDYIDRIIVAILETNPSILEVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001162869
ORF Size	3276 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001162869.1, NP_001156341.1
RefSeq Size	5015 bp
RefSeq ORF	3279 bp
Locus ID	215445
UniProt ID	Q8CHD8
MW	123.7 kDa
Gene Summary	Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis (By similarity). Also required for the structural integrity of the endosomal recycling compartment during interphase. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).[UniProtKB/Swiss-Prot Function]

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