United States
Germany
Japan
United Kingdom
China
Cdc27 (NM_145436) Mouse Tagged Lenti ORF Clone
CAT＃: MR223434L3
- LentiORF®
Lenti ORF clone of Cdc27 (Myc-DDK-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27)
"NM_145436" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,120.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI452358; APC3; BC023187 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR223434).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145436 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145436.2, NP_663411.2 |
| RefSeq Size | 5783 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 217232 |
| UniProt ID | A2A6Q5 |
| Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221891 | Cdc27 (untagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug) |
CNY 10,550.00 |
|
| MG223434 | Cdc27 (tGFP-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug) |
CNY 7,980.00 |
|
| MR223434 | Cdc27 (Myc-DDK-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27) |
CNY 6,040.00 |
|
| MR223434L4 | Lenti ORF clone of Cdc27 (mGFP-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27) |
CNY 9,120.00 |
