Cdc27 (NM_145436) Mouse Untagged Clone

CAT#: MC221891

Cdc27 (untagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug)



  "NM_145436" in other vectors (4)

CNY 10,550.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI452358; APC3; BC023187
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221891 representing NM_145436
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCGCTAAACCACTATGCTTACCGAGATG
CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG
TTACTACCGCTCAGGAAAGGCTTATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACCACCCCACAG
TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAGATCTTATCTG
GTGGAGTGTTTAATAAGCAGAAAAGCCATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCAC
TCTTTCCTTGTTGGGACATGTGTATTGCAAGACAGATCGGCTTGCCAAAGGGTCAGAATGTTACCAAAAG
AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACTTGTACAACTCT
AGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACACCCCAAGACACAATT
GAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
ACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGCCATATTATCTAAACA
GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
CACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGTCTGTTGCAAGAATGGGCCAAAC
TGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGTCGAGAGGTCACACCAGTACTTGTTGCACAAACA
CAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATCACATCTCCCCCAAACG
CATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
AAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGACTAACT
CAGCCCAGCATAAACGATAGTCTGGAAATTACAAAACTGGACTCCTCTATCATTTCAGAAGGGAAAATAA
CCACAGTCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCT
TCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATCAATATTTTGAGC
CATCTACCTTCTCATCACTACAGTACTGGTTGGGTCCTATGCCAGATTGGACGGGCTTATTTTGAACTTT
CAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAGTTTCAGAGTTGAAGGAAT
GGAGATCTACTCTACAACACTCTGGCATCTTCAGAAAGACGTTGCTCTTTCAGTTCTTTCAAAAGATTTA
ACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGAGAGC
ATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTCGATCCAAATTACGCTTACGCCTATACTCT
ATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGA
GTAAATCCCAGACATTACAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCAAGAGAAGTTCAGCC
TCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGG
AGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATCGAT
CCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGGTTTTATTTGCAAATGAAAAATACAAGTCTGCTT
TACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAAAGAGTCCCTCGTTTACTTCCTAATAGGAAAGGT
TTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAA
GGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCAATAACCC
AGGAGGAACAGATCATGGGGACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACACAACT
TCATGCGGCGGAAAGTGACGAATTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145436
Insert Size 2478 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145436.2, NP_663411.2
RefSeq Size 5783 bp
RefSeq ORF 2478 bp
Locus ID 217232
UniProt ID A2A6Q5
Gene Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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