Ncr1 (NM_010746) Mouse Tagged ORF Clone

CAT#: MR223898

  • TrueORF®

Ncr1 (Myc-DDK-tagged) - Mouse natural cytotoxicity triggering receptor 1 (Ncr1)

ORF Plasmid: DDK tGFP



  "NM_010746" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CD335 Rabbit pAb
    • 100 ul

CNY 2,640.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Ly94; NKp46
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR223898 representing NM_010746
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCAACACTCACTGCCCTACTGTGCCTTGGGCTATGTCTGAGCCAGAGGATCAACACTGAAAAGG
AGACTCTCCCGAAACCCATCATCTGGGCCAAACCCAGCATCATGGTCACAAATGGAAACTCGGTGAACAT
CTGGTGTCAGGGGGCTCAGAGTGCTTCTGAGTATCAACTGTACTTTGAAGGAAGCTTTTTTGCCTTGGAG
AGACCAAAGCCATCGAGATCGATGAATAAAGTTAGGTTCTTCATTTCACAAATGACCTCACATACTGCAG
GGATCTACACCTGCTTCTATCAGAGTGGAGAGCTCTGGTCAAAGTCGAGCAACCCCCTGAAACTGGTAGT
AACTGGTCTGTATGACACACCCAACCTCTGGGTTTATCCACAGCCTGAGGTAACCTTGGGGGAGAATGTT
ACCTTCTTTTGCCAACTGAAGACTGCCACAAGCAAATTCTTTCTGCTCAAGGAGAGAGGATCCAACCACA
TCCAGAATAAATATGGGAACATCCAAGCAGAGTTTCCCATGGGCCCCGTGACTAGGGCTCACAGAGGGAC
ATACAGATGTTTTGGCTCTTACAACGACTATGCATGGTCTTTTCCCAGTGAGCCTGTGACACTACTCATC
ACAGGAGGTGTTGAGAACAGCAGCCTTGCACCTACCGACCCTACTTCTTCTCTTGATTATTGGGAGTTTG
ACCTTTCAACCAATGAATCAGGATTACAAAAGGACTCCGCCTTCTGGGATCACACAACCCAGAATCTCAT
TCGAATTGGTCTGGCATGCATAATCCTGATAACTCTAGTATGGCTTTTGACTGAAGACTGGCTCAGCAAG
AGGAAAGATCATGAAGAGGCCAACAGATTAACAAATTGGGAATGCAGGAGAAGATGGAGAATGCAGCATT
ACTTTGAAGAGGAACAAAGAAATGCAATATCTATGATGGAACTGAAGGCAACTCCTGGGGCCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR223898 representing NM_010746
Red=Cloning site Green=Tags(s)

MLPTLTALLCLGLCLSQRINTEKETLPKPIIWAKPSIMVTNGNSVNIWCQGAQSASEYQLYFEGSFFALE
RPKPSRSMNKVRFFISQMTSHTAGIYTCFYQSGELWSKSSNPLKLVVTGLYDTPNLWVYPQPEVTLGENV
TFFCQLKTATSKFFLLKERGSNHIQNKYGNIQAEFPMGPVTRAHRGTYRCFGSYNDYAWSFPSEPVTLLI
TGGVENSSLAPTDPTSSLDYWEFDLSTNESGLQKDSAFWDHTTQNLIRIGLACIILITLVWLLTEDWLSK
RKDHEEANRLTNWECRRRWRMQHYFEEEQRNAISMMELKATPGAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010746
ORF Size 975 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010746.3, NP_034876.2
RefSeq Size 1463 bp
RefSeq ORF 978 bp
Locus ID 17086
UniProt ID Q8C567
MW 37.7 kDa
Gene Summary Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...