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Pde3a (NM_018779) Mouse Tagged ORF Clone
CAT＃: MR224945
- TrueORF®
Pde3a (Myc-DDK-tagged) - Mouse phosphodiesterase 3A, cGMP inhibited (Pde3a)
ORF Plasmid: tGFP
"NM_018779" in other vectors (4)
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CNY 7,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A930022O17Rik; C87899 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224945 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCGGGGCGAGGCCGCCCAAGACCTAGCCAAGCCGGGCCTCGGAGGGGCGAGTCCCGCCCGTG TGGCAAGAGGGAACCACCGCCATCGTGGCGAGTCCTCTCCATCCCCTCGGGGCTCCGGCTGTTGTTGGAG AGCCCTGGCGCTGCAGCCGCTCCGGCGCTCGCCTCAACTCTCCTCGGCGCTGTGCGCGGGCTCCCTGTCC GTGCTGCTGGCGCTGCTGGTGAGGCTGGTCGGCGGGGAGGTCGGAGGCGAGCTGGAGAAGAGTCAGGAGG CGGCGGCCGAGGAGGAGGAGGAGGAAGGAGCCCGAGGGGGCGTCTTCCCGGGCCCTCGGGGAGGTGCTCC CGGGGGCGGCGCGCAGCTCAGCCCTTGGCTGCAGCCGGCCGCGCTGCTCTTCAGTCTCCTGTGCGCCTTC TTCTGGATGGGCTTGTGCCTGCTGCGCGCCGGGGTGCGCCTGCCTCTGGCCGTCGCGCTGTTGGCCGCCT GCTGCGCCGGGGAAGCGCTGGTGCAGCTCTCCTTGGGCGTGGGGGACGGTCGCCTGCTGTCGCTGCCCGC CGCGGGGGTCCTGCTCAGCTGCCTAGGTGGCGCGACATGGCTGGTGCTGAGGCTGAGGCTGGGCGTCCTC ATGGTCGCCTGGACTAGCGTGCTTAGGACGGTGGCCCTGGTTTCTCTGGAGAGGTTCAAGGTCGCCTGGA GACCTTACCTGGCATACTTGGCGGCCGTGCTTGGGCTGCTGCTGGCCAGGTACGCCGAGCAGATCCTGCC GCAGTGCTCCGGGCCGGCTCCGCCCAGGGAGCGGTTTGGGTCCCAGTTGAGCGCGAGGACCAAGGAAGAG ATTCCGGGGTGGAAGAGGAGGAGGCGGTCCAGCTCGGTGGTGGCCGGCGAGATGTCCGGCTGCAGTGGCA AGTCTCACCGGAGGACCTCCCTGCCCTGCATACCCAGGGAGCAGCTCATGGGCCATTCAGAATGGGACCA CAAGAGAGGGCCACGAGGATCCCAGTCAGGAACCAGCATCACTGTGGATATTGCTGTGATGGGTGAGGCC CATGGCCTCATTACCGACCTCCTGGCCGACCCTTCTCTGCCCCCGAATGTGTGTACATCCTTGAGGGCCG TAAGCAACTTGCTCAGCACCCAGCTCACCTTCCAGGCCATCCACAAGCCCAGAGTGAACCCCACCGTGAC CTTCAGTGAGAACTATACCTGCTCGGACTCTGAGGAAGGCTTGGAGAAAGACAAGCAGGCCATTTCCAAG CGCCTGAGAAGAAGTTTGCCTCCAGGCTTACTGAGAAGAGTCTCGTCAACTTGGACAACTACCACCTCTG CCACAGGTCTGCCCACCTTGGAGCCTGCGCCAGTGCGGAGGGACCGCAGTGCCAGCATAAAGCCGCACGA AGCCCCTTCACCCAGTGCTGTCAATCCTGACTCTTGGAATGCCCCAGGGTTGACGACTCTCACCAAAAGC AGATCCTTCACTTCATCCTATGCTGTCTCTGCAGCTAACCATGTAAAAGCTAAAAAGCAAAACAGGCCAG GTGGCCTTGCTAAAATTTCCCCCGTCCCCTCACCCTCCTCCTCGCCTCCCCAGGGATCACCTGCCAGCAG TCCTGTCAGCAACAGTGCTTCACAACAGTTCCCAGAATCCCCTGAAGTAACCATCAAGCGAGGGCCTGGA TCTCACAGGGCCTTAACTTACACCCAAAGTGCCCCTGACCTGTCCCCTCAGATCCCACCCCCGTCTGTCA TATGTAGCAGCTGTGGCAGACCTTATTCGCAAGGGAATCCTGCTGATGGACCCTCAGAGAGAAGTGGCCC AGCCATGCTGAAACCAAACAGAACAGATGACACTTCTCAAGTTACCTCCGATTATGAGACCAACAACAAC AGTGACAGCAGTGACATTCTGCAGAATGAAGAGGAAGCTGAGTGCCAGAGAGAACCCCAGAGGAAAGCAT CGGCTTGTGGCACCTATACTTCCCAGACCATGATCTTCCTGGACAAACCAATTCTTGCTCCAGAACCCCT GGTCATGGATAACCTGGACTCAATTATGGATCAGTTGAACACCTGGAATTTTCCAATTTTTGATTTAATG GAAAATATAGGAAGAAAATGTGGCCGTATTCTGAGCCAGGTATCATACAGACTGTTTGAAGACATGGGGC TCTTTGAAGCCTTTAAAATCCCGGTTAGGGAGTTTATGAATTACTTTCATGCTTTGGAGATCGGATACAG GGACATTCCTTATCATAACAGAATCCATGCCACCGATGTTCTACACGCTGTGTGGTATCTCACAACACAG CCGATTCCTGGCCTCCCAAGTGTGATTGGTGATCACGGCTCAGCAAGCGACTCCGATTCTGACAGTGGAT TTACACACGGACACATGGGATATGTGTTTTCAAAAATGTATCACGTGCCAGATGACAAATATGGATGCCT GTCTGGGAATATTCCAGCCCTAGAGTTGATGGCTCTGTACGTTGCTGCAGCCATGCATGACTACGATCAC CCAGGGAGGACAAATGCCTTCCTGGTTGCCACTAGTGCCCCTCAGGCGGTGCTATACAACGACCGTTCTG TTCTGGAGAACCATCACGCCGCTGCAGCCTGGAATCTCTTCATGTCCCGGCCAGAGTATAACTTCTTAGT TAACCTGGACCACGTGGAATTCAAGCACTTCCGCTTCCTAGTCATTGAAGCGATTTTGGCTACTGACCTG AAGAAACACTTTGACTTTGTAGCCAAATTTAACGCCAAGGTAAACGATGATGTCGGGATAGATTGGACCA ATGAAAACGACCGTCTCCTGGTGTGTCAGATGTGTATAAAGCTGGCTGACATCAACGGGCCTGCTAAATG CAAAGAACTCCACCTGCGGTGGACAGAGGGCATCGCCAGCGAGTTTTATGAGCAGGGCGATGAAGAAGCC AGCCTCGGCTTGCCCATAAGTCCCTTTATGGACCGCTCTGCCCCGCAGCTGGCCAATCTTCAGGAATCCT TTATCTCACACATCGTGGGTCCTCTCTGCCACTCCTATGACTCTGCGGGACTCATGCCAGGAAAATGGGT AGATGACAGTGATGATTCCGGAGACACCGATGACCCGGAAGAAGAGGAGGAAGAGGCTGAAACACCACAC GAGGACGAAGCCTGTGAAAGCAGTATAGCTCCCCGAAAGAAAAGTTTCAAAAGAAGGAGAATCTACTGCC AAATAACTCAGCACCTTCTGCAGAACCACATGATGTGGAAGAAAGTGATTGAAGAGGAACAGTGTTTATC AGGCACAGAGAACCAGTCCCTGGACCAGGTCCCTCTGCAGCATCCCTCAGAGCAGATCCAGGCTATCAAG GAGGAAGAGGAAGAGAAGGGGAAGCCAAGAGCAGAGGAGACCCTGGCTCCACAGCCAGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224945 protein sequence
Red=Cloning site Green=Tags(s) MAVRGEAAQDLAKPGLGGASPARVARGNHRHRGESSPSPRGSGCCWRALALQPLRRSPQLSSALCAGSLS VLLALLVRLVGGEVGGELEKSQEAAAEEEEEEGARGGVFPGPRGGAPGGGAQLSPWLQPAALLFSLLCAF FWMGLCLLRAGVRLPLAVALLAACCAGEALVQLSLGVGDGRLLSLPAAGVLLSCLGGATWLVLRLRLGVL MVAWTSVLRTVALVSLERFKVAWRPYLAYLAAVLGLLLARYAEQILPQCSGPAPPRERFGSQLSARTKEE IPGWKRRRRSSSVVAGEMSGCSGKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEA HGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISK RLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKS RSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPG SHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNN SDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLM ENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQ PIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDH PGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDL KKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEA SLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPH EDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIK EEEEEKGKPRAEETLAPQPDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018779 |
| ORF Size | 3426 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018779.2 |
| RefSeq Size | 4205 bp |
| RefSeq ORF | 3426 bp |
| Locus ID | 54611 |
| UniProt ID | Q9Z0X4 |
| MW | 124.5 kDa |
| Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223711 | Pde3a (untagged) - Mouse phosphodiesterase 3A, cGMP inhibited (Pde3a), (10ug) |
CNY 14,730.00 |
|
| MG224945 | Pde3a (tGFP-tagged) - Mouse phosphodiesterase 3A cGMP inhibited (Pde3a), (10ug) |
CNY 9,220.00 |
|
| MR224945L3 | Lenti ORF clone of Pde3a (Myc-DDK-tagged) - Mouse phosphodiesterase 3A, cGMP inhibited (Pde3a) |
CNY 10,260.00 |
|
| MR224945L4 | Lenti ORF clone of Pde3a (mGFP-tagged) - Mouse phosphodiesterase 3A, cGMP inhibited (Pde3a) |
CNY 10,260.00 |
