Tnfrsf1a (NM_011609) Mouse Tagged ORF Clone

CAT#: MR226545

  • TrueORF®

Tnfrsf1a (Myc-DDK-tagged) - Mouse tumor necrosis factor receptor superfamily, member 1a (Tnfrsf1a)

ORF Plasmid: DDK tGFP



  "NM_011609" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 3,656.00

CNY 4,090.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CD120; CD120a; FPF; p5; p55; p55-R; TN; Tnf; TNF-; TNF-a; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFal; TNFalpha-R1; TNFAR; TNFR; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226545 representing NM_011609
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCTCCCCACCGTGCCTGGCCTGCTGCTGTCACTGGTGCTCCTGGCTCTGCTGATGGGGATACATC
CATCAGGGGTCACTGGACTAGTCCCTTCTCTTGGTGACCGGGAGAAGAGGGATAGCTTGTGTCCCCAAGG
AAAGTATGTCCATTCTAAGAACAATTCCATCTGCTGCACCAAGTGCCACAAAGGAACCTACTTGGTGAGT
GACTGTCCGAGCCCAGGGCGGGATACAGTCTGCAGGGAGTGTGAAAAGGGCACCTTTACGGCTTCCCAGA
ATTACCTCAGGCAGTGTCTCAGTTGCAAGACATGTCGGAAAGAAATGTCCCAGGTGGAGATCTCTCCTTG
CCAAGCTGACAAGGACACGGTGTGTGGCTGTAAGGAGAACCAGTTCCAACGCTACCTGAGTGAGACACAC
TTCCAGTGCGTGGACTGCAGCCCCTGCTTCAACGGCACCGTGACAATCCCCTGTAAGGAGACTCAGAACA
CCGTGTGTAACTGCCATGCAGGGTTCTTTCTGAGAGAAAGTGAGTGCGTCCCTTGCAGCCACTGCAAGAA
AAATGAGGAGTGTATGAAGTTGTGCCTACCTCCTCCGCTTGCAAATGTCACAAACCCCCAGGACTCAGGT
ACTGCGGTGCTGTTGCCCCTGGTTATCTTGCTAGGTCTTTGCCTTCTATCCTTTATCTTCATCAGTTTAA
TGTGCCGATATCCCCGGTGGAGGCCCGAAGTCTACTCCATCATTTGTAGGGATCCCGTGCCTGTCAAAGA
GGAGAAGGCTGGAAAGCCCCTAACTCCAGCCCCCTCCCCAGCCTTCAGCCCCACCTCCGGCTTCAACCCC
ACTCTGGGCTTCAGCACCCCAGGCTTTAGTTCTCCTGTCTCCAGTACCCCCATCAGCCCCATCTTCGGTC
CTAGTAACTGGCACTTCATGCCACCTGTCAGTGAGGTAGTCCCAACCCAGGGAGCTGACCCTCTGCTCTA
CGAATCACTCTGCTCCGTGCCAGCCCCCACCTCTGTTCAGAAATGGGAAGACTCCGCCCACCCGCAACGT
CCTGACAATGCAGACCTTGCGATTCTGTATGCTGTGGTGGATGGCGTGCCTCCAGCGCGCTGGAAGGAGT
TCATGCGTTTCATGGGGCTGAGCGAGCACGAGATCGAGAGGCTGGAGATGCAGAACGGGCGCTGCCTGCG
CGAGGCTCAGTACAGCATGCTGGAAGCCTGGCGGCGCCGCACGCCGCGCCACGAGGACACGCTGGAAGTA
GTGGGCCTCGTGCTTTCCAAGATGAACCTGGCTGGGTGCCTGGAGAATATCCTCGAGGCTCTGAGAAATC
CCGCCCCCTCGTCCACGACCCGCCTCCCGCGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226545 representing NM_011609
Red=Cloning site Green=Tags(s)

MGLPTVPGLLLSLVLLALLMGIHPSGVTGLVPSLGDREKRDSLCPQGKYVHSKNNSICCTKCHKGTYLVS
DCPSPGRDTVCRECEKGTFTASQNYLRQCLSCKTCRKEMSQVEISPCQADKDTVCGCKENQFQRYLSETH
FQCVDCSPCFNGTVTIPCKETQNTVCNCHAGFFLRESECVPCSHCKKNEECMKLCLPPPLANVTNPQDSG
TAVLLPLVILLGLCLLSFIFISLMCRYPRWRPEVYSIICRDPVPVKEEKAGKPLTPAPSPAFSPTSGFNP
TLGFSTPGFSSPVSSTPISPIFGPSNWHFMPPVSEVVPTQGADPLLYESLCSVPAPTSVQKWEDSAHPQR
PDNADLAILYAVVDGVPPARWKEFMRFMGLSEHEIERLEMQNGRCLREAQYSMLEAWRRRTPRHEDTLEV
VGLVLSKMNLAGCLENILEALRNPAPSSTTRLPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011609
ORF Size 1362 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011609.4, NP_035739.2
RefSeq Size 2186 bp
RefSeq ORF 1365 bp
Locus ID 21937
UniProt ID P25118
MW 50.6 kDa
Gene Summary This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mice lacking a functional copy of this gene exhibit impaired immune function. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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