Prdm2 (NM_001081355) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4833427P12Rik; E330024L24Rik; Gm1033; Gm19732; KMT8; Riz; Riz1; Znfpr1c1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227154 representing NM_001081355 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCAGAACACTGAGTCTGTGGCAGTCACTGAGACTCTGGCTGAGGTACCTGAACACGTGCTTCGAG GACTTCCAGAGGAAGTAAGACTTTTACCATCTGCGGTCGACAAGACTCGGATTGGTGTCTGGGCCACTAA ACCAATTTTAAAAGGGAAAAAGTTTGGGCCATTTGTTGGTGATAAGAAGAAGAGATCCCAGGTTAGGAAT AATGTATACATGTGGGAGGTGTACTACCCAAATTTGGGGTGGATGTGCATTGATGCCACTGATCCGGAGA AGGGCAACTGGCTCCGCTATGTGAACTGGGCTTGCTCAGGAGAAGAACAGAATTTATTTCCACTGGAAAT CAACAGAGCCATTTACTATAAAACCTTAAAGCCAATCGCGCCTGGCGAGGAGCTCCTGGTCTGGTACAAT GGGGAAGACAACCCCGAGATAGCAGCTGCGATTGAGGAAGAGCGAGCCAGCGCCCGGAGCAAGCGGAGCT CCCCGAAGAGCCGGAGAGGGAAGAAGAAATCACAGGAGAATAAAAACAAAGGCGTCAGAACCCAGGCTGC AGCGCGGAAGGCGAGCGAGCTGGACTCCACCTCTGCAAACATGAGGGGCTCTGCAGAAGGTCCAAAAGAA GAGGATGAGAGGCCTTTGGCTTCTGCACCTGAGCAGCCAGCCCTTCTGCCAGAGGTGGTAAGCCAGGATG CAGTTCCACAGGTGGCCATCCCTCTTCCTGCCTGCGAGTCACAGCCAGGGGCAGATGGGAAACAAGAAGT CACAGACTGTGAGGTCAACAATATGAAGGAAGAGGAGGAGGAGGAGGAGGAGGAGCTGGAAGAGGAAGAA GAGGAGGAGGAGTTGGGAGAAGATGGGGAGGAAGAAGCAGACATGCCGAATGAAAGCTCTGTGAAAGAAC CAGAAATACGGTGTGAAGAAAAGCCAGAAGATTTACTGGAAGAGCCACGGAGTGTGCCAACTGAAACTTC TGAAGGCTCCCCAGGCGCCACCCCTCCTCCCCACGCTCCCAGGGCTAGAGAGGAGGCCAACGGCGAGGGG CTTGAAACATTCATGTTTCCATGTCAGCATTGTGAAAGGAAATTTGCAACGAAGCAGGGGCTAGAACGTC ACATGCACATCCACATTTCTACAATCAACCACGCTTTCAAGTGCAAGTACTGTGGGAAACCATTTGGCAC ACAGATCAACAGGAGGCGGCATGAAAGGCGCCACGAAACAGGGTTGAAGAGAAGACCCAGCATGGCATTA CAATCCTCAGAGGACCCAGACGATGGCAAGGGGGAAAATGTTACTTCTAAAGATGAGTCGAGTCCACCTC AACTTGGGCAAGACTGTTTGATATTGAACTCAGAGAAAACCTCACAGGAAATACTGAATTCATCTTTTGT GGAAGAGAATGGTGAAGTTAAAGAACTTCACCCATGCAAATACTGCAAAAAGGTATTTGGAACTCACACC AATATGAGACGACATCAGCGTAGAGTTCACGAACGCCACCTGATTCCCAAAGGTGTCCGGCGAAAAGGAG GCCTCCTAGAAGAGCCGCAGCCACCTGCAGAGCAGGCCCCGCCCTCCCAGAATGTCTATGTACCAAGCAC AGAGCCCGAGGAGGACGGGGACGCAGATGATGTGTATATCATGGACATTTCTAGCAACATCTCTGAGAAC CTAAATTACTATATTGATGGTAAGATTCAGACCAACAACAGCACTAGTGATTGTGATGTGATTGAGATGG AGTCTAACTCTGCACACTTGTATGGCATAGACTGTCTGCTCACTCCAGTAACTGTAGAGATTACTCAAAA CATAAAGAGCACTCAGGGCTCTGTGACAGATGATCTTCTTAAAGAGTCTCCCAGTAGCACAAATTGCGAG TCTAAGAAACGGAGGACTGCCAGTCCACCTGTACTCCCTAAAATTAAAGCAGAGACAGATTCTGATTCCA CGGCACCCTCGTGTTCCTTAAGTCTGCCTCTGAGCATATCAACAACAGAGGTGGTGTCTTTCCATAAAGA GAAGGGTGTCTATTTGTCTTCAAAGCTCAAGCAGCTTCTTCAGACCCAGGACAAACTGACTCCTCCTGCA GGGATTTCAACAGCTGAGATTCCTAAGTTAGGTCCCGTGTGTGTATCTGCTCCTGCATCCATGTTAGCTG TGACTTCTAGTAGGTTTAAGAGACGCACCAGCTCTCCCCCCAGTTCTCCACAGCACAGCCCTGCCCTTCG AGACTTTGGGAAACAGAGTGATGGGAAAGCAGCATGGACAGACACAGCTCTGACTTCCAAGAAACCGAAG TTAGAAAGTCGTAGTGACTCACCAGCGTGGAGTTTGTCTGGGAGAGATGAGAGAGAAACTGGAAGCCCTC CTTGTTTTGATGAATACAAAATATCAAAAGAATGGGCAGCCAGTTCTACTTTCAGCAGCGTGTGCAACCA ACAGCCGTTGGATTTATCCAGCGGTGTCAAACAGAAGTCAGAGGGCACAGGCAAGACTCCAGTCCCATGG GAATCTGTATTGGATCTCAGTGTGCATAAAAAGCCTTGTGATTCTGAAGGCAAGGAATTCAAAGAGAACC ATTTGGCACAGCCGGCTGCAAAGAAGAAAAAACCAACCACCTGCATGCTACAAAAGGTTCTTCTCAATGA ATATAATGGTGTTAGCTTACCTACAGAAAGCACACCAGAGGCGACCAGGAGCCCAAGTCCTTGTAAATCA CCAGACACACAGCCAGATCCTGAACTTGCTACTGACTCGAGTTGCTCAGCCCCCACTGCTGAGTCTCCAC CTGAGGTTGTTGGCCCTTCATCACCCCCTCTCCAGGCAGCCTCCCTGTCCTCTGGTCAGCTGCCTCCTCT CTTGATCCCCACAGAGCCTTCTTCCCCTCCGCCCTGCCCTCCTGTGTTAACTGTTGCCACTCCACCACCT CCCCTCCTTCCGACTGTCCCTCTCCCCAACCCCTCTTGTGATGCCTCTCCTCAACAGTGCCCCTCTCCAT TCTCAAATGCCACTGCTCAGTCTCCTCTTCCGATTCTCTCCCCAACAGTGTCCCCCTCTCCCTCTCCTAT TCCTCCTGTAGAGCCACTCATGTCTGCTGCTTCCCCTGGTCCTCCGACACTTTCCTCCTCTTCCTCCTCC TCTTCCTCCTCTTCCTTCTCTTCCTCTTCATGTTCCTCCTCCTCCCCCTCACCACCCCCTCTCTCAGCAG TGTCATCTGTGGTGTCCTCTGGGGACAACCTGGAAGCATCTCTCCCTGCAATAACTCTCAAACAGGAGGA GTCAGAGAGTGAAGGTCTGAAACGCAAGGAAGAGGCCCCAGCTGCAGGTGGGCAGAGCGCTGTCCAAGAG ACATTCAGCAGAAACTTCGTCTGCAATGTGTGCGCATCACCTTTTCTTTCCATTAAAGACCTAACCAAAC ATTTGTCTGTCCATGCTGAAGAATGGCCCTTCAAATGTGAGTTTTGTGTGCAGCTTTTTAAGGGTAAGAC CGATCTATCAGAGCACCGATTTCTGCTTCATGGAGTCGGAAATATCTTTGTGTGTTCTGTATGTAAGAAA GAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGATCTCCACCCAGATGAGTTATGCACACACC ACGAGTTTGAAAGTGGGACCCTGAGGCCCCAGAACTTCACAGACCCCAGCAAGGCCCATGTGGAGCATAT GCCGAGTTTGCCAGAAGAGCCTGCGGAAGCTTCTAGAGAGGAGGAGTTAAATGACTCCTCTGAAGAGCTT TACACGACCATCAAAATAATGGCTTCCGGAATAAAGACGAAAGAGCCAGATGTTCGACTTGGTCTCAACC AGCATTACCCGAGCTTTAAGCCACCTCCATTTCAGTACCACCATCGAAACCCTATGGGGATTGGGGTAAC AGCCACAAACTTCACCACCCACAATATCCCACAGACTTTCACTACTGCCATCCGCTGCACAAAGTGTGGC AAGAGTGTTGATAACATGCCTGAGCTGCATAAGCACATCTTGGCGTGTGCATCTGCAAGTGACAAGAAGA GGTACACTCCTAAGAAAAACCCAGTGCCGCTGAGACAGACGGTGCAGCCCAAAAATGGAGTGCTGGTTTT AGACAACTCTGGGAAAAATGCCTTCAGACGGATGGGGCAGCCTAAGAGACTGAGCTTCAATGTTGAACTC AGCAAAATGTCTCCAAATAAGCTCAAGCTAAATGCATTGAAGAAAAAAAATCAGCTGGTACAGAAAGCGA TCCTTCAGAAGAACAGATCTGCGAAGCAGAAGGCAGACCTGAGGGACACTTCCGAGGCGTCCTCACATAT CTGCCCTTACTGTGACAGGGAGTTCACGTACATCGGCAGCCTGAATAAGCATGCTGCTTTCAGCTGTCCT AAAAAACCCCTTTCTCCTTCCAAAAGAAAAGTTTCTCATTCATCTAAGAAAGGTGGCCACACATCATCTG CCAGCAGTGACAGAAACAGTAGTAGCAATCCCCGGAGGCGCACCGCAGATACGGAGATCAAGATGCAGAG CATGCAGGCACCCTTGGGCAAGACCCGAGCTCGGAGTACAGGCCCTGCCCAAGCCTCGCTGCCCTCCTCA TCCTTCAGATCCAGACAGAATGTCAAGTTTGCAGCTTCAGTCAAGTCCAAAAAAGCAAGCTCTTCATCCT TGAGGAATTCTAGTCCCGTAAGAATGGCTAAGATTACTCATGTTGAGGGCAAAAAATCCAAAGCTGTTGC CAAGAGTCATTCTACTCAGCTCTCAAGCAAAGCATCCCGAAGCCTGCACGTGAAGGTGCAGAAGAGCAAA GCTGTTCTACAAAGCAAGACTGCCCTGGCCAGCAAGAAAAGAACAGACCGGTTCATAGTAAAATCTAGAG AGCGGAGCGGGGGCCCAATCACCCGAAGCCTTCAGCTGGCAGCTGCTGCGGACCTGAGTGAAAGCAGGAG AGAGGACAGCAGTGCCAGGCATGAGCTGAAGGACTTGAGCTACAGCCTCCGCCTGGCATCGCGATGCGCC TCATCAACAGCCCCTTATATCACCAGACAATGCAGAAAGGTCAAGGCTGCAGCAGCAACTCAGTTCCAGG GACCCTTCTTCAAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227154 representing NM_001081355 Red=Cloning site Green=Tags(s) MHQNTESVAVTETLAEVPEHVLRGLPEEVRLLPSAVDKTRIGVWATKPILKGKKFGPFVGDKKKRSQVRN NVYMWEVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINRAIYYKTLKPIAPGEELLVWYN GEDNPEIAAAIEEERASARSKRSSPKSRRGKKKSQENKNKGVRTQAAARKASELDSTSANMRGSAEGPKE EDERPLASAPEQPALLPEVVSQDAVPQVAIPLPACESQPGADGKQEVTDCEVNNMKEEEEEEEEELEEEE EEEELGEDGEEEADMPNESSVKEPEIRCEEKPEDLLEEPRSVPTETSEGSPGATPPPHAPRAREEANGEG LETFMFPCQHCERKFATKQGLERHMHIHISTINHAFKCKYCGKPFGTQINRRRHERRHETGLKRRPSMAL QSSEDPDDGKGENVTSKDESSPPQLGQDCLILNSEKTSQEILNSSFVEENGEVKELHPCKYCKKVFGTHT NMRRHQRRVHERHLIPKGVRRKGGLLEEPQPPAEQAPPSQNVYVPSTEPEEDGDADDVYIMDISSNISEN LNYYIDGKIQTNNSTSDCDVIEMESNSAHLYGIDCLLTPVTVEITQNIKSTQGSVTDDLLKESPSSTNCE SKKRRTASPPVLPKIKAETDSDSTAPSCSLSLPLSISTTEVVSFHKEKGVYLSSKLKQLLQTQDKLTPPA GISTAEIPKLGPVCVSAPASMLAVTSSRFKRRTSSPPSSPQHSPALRDFGKQSDGKAAWTDTALTSKKPK LESRSDSPAWSLSGRDERETGSPPCFDEYKISKEWAASSTFSSVCNQQPLDLSSGVKQKSEGTGKTPVPW ESVLDLSVHKKPCDSEGKEFKENHLAQPAAKKKKPTTCMLQKVLLNEYNGVSLPTESTPEATRSPSPCKS PDTQPDPELATDSSCSAPTAESPPEVVGPSSPPLQAASLSSGQLPPLLIPTEPSSPPPCPPVLTVATPPP PLLPTVPLPNPSCDASPQQCPSPFSNATAQSPLPILSPTVSPSPSPIPPVEPLMSAASPGPPTLSSSSSS SSSSSFSSSSCSSSSPSPPPLSAVSSVVSSGDNLEASLPAITLKQEESESEGLKRKEEAPAAGGQSAVQE TFSRNFVCNVCASPFLSIKDLTKHLSVHAEEWPFKCEFCVQLFKGKTDLSEHRFLLHGVGNIFVCSVCKK EFAFLCNLQQHQRDLHPDELCTHHEFESGTLRPQNFTDPSKAHVEHMPSLPEEPAEASREEELNDSSEEL YTTIKIMASGIKTKEPDVRLGLNQHYPSFKPPPFQYHHRNPMGIGVTATNFTTHNIPQTFTTAIRCTKCG KSVDNMPELHKHILACASASDKKRYTPKKNPVPLRQTVQPKNGVLVLDNSGKNAFRRMGQPKRLSFNVEL SKMSPNKLKLNALKKKNQLVQKAILQKNRSAKQKADLRDTSEASSHICPYCDREFTYIGSLNKHAAFSCP KKPLSPSKRKVSHSSKKGGHTSSASSDRNSSSNPRRRTADTEIKMQSMQAPLGKTRARSTGPAQASLPSS SFRSRQNVKFAASVKSKKASSSSLRNSSPVRMAKITHVEGKKSKAVAKSHSTQLSSKASRSLHVKVQKSK AVLQSKTALASKKRTDRFIVKSRERSGGPITRSLQLAAAADLSESRREDSSARHELKDLSYSLRLASRCA SSTAPYITRQCRKVKAAAATQFQGPFFKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081355
ORF Size	5127 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081355.3, NP_001074824.3
RefSeq Size	7323 bp
RefSeq ORF	5130 bp
Locus ID	110593
MW	187.7 kDa

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