Syt6 (NM_001276680) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3110037A08Rik; AW048930; sytVI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229363 representing NM_001276680 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTGGAGGAAGAAAGAGGCCTCCAGCCCTTCTTCTGCTAACCCTGCTTCAGAGACTCTCCAGAGCC CCAGCTCCAGAGGCAACATGGCGGATAAGCTGAAGGACCCCAGTGCCCTGGGCTTCCTGGAGGCAGCTGT GAAGATCAGCCACACTTCCCCAGACATCCCAGCCGAGGTGCAGATGTCGGTCAAAGAGCACATCATGCGC CACACAAAGCTGCAGCGACAGACCACAGAGCCAGCATCCTCCACCAGGCACACATCCTTCAAGCGCCACC TGCCACGACAGATGCATGTCTCCAGCGTGGACTATGGCAATGAGCTGCCGCCGGCTGCCGCCGAGCAGCC CACCAGTATTGGCCGTATCAAGCCTGAGCTTTACAAGCAGAAGTCAGTGGATGGGGATGATGCCAAGTCG GAGGCCGCCAAGAGCTGTGGGAAGATCAACTTCAGCCTCCGCTATGACTATGAAAGCGAGACGCTGATTG TGCGCATCCTGAAGGCCTTTGACCTCCCTGCCAAGGACTTTTGCGGAAGTTCTGACCCTTATGTTAAGAT CTACCTCCTGCCTGACCGCAAGTGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTT GATGAGAACTTCCACTTCCCCGTGCCCTACGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTTG ACTTTGACCGCTTCTCCCGCCATGACATGATTGGGGAGGTCATCCTGGACAATCTCTTTGAGGCCTCTGA CCTGTCCCGGGAGACCTCCATCTGGAAGGACATCCAGTACGCTACTAGTGAAAGTGTGGACTTGGGAGAG ATCATGTTCTCCCTCTGCTACCTGCCCACAGCAGGCAGGCTCACCCTCACAGTGATCAAGTGTCGAAATC TCAAAGCAATGGACATCACAGGCTACTCAGAAAAATCCCAAGGCCACAGAAGGATCCAAGTTCAGGATAC TCATCCGCTGTGGAGACTGCAGGCAATGAAAAGTCTGGTCGTCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229363 representing NM_001276680 Red=Cloning site Green=Tags(s) MPWRKKEASSPSSANPASETLQSPSSRGNMADKLKDPSALGFLEAAVKISHTSPDIPAEVQMSVKEHIMR HTKLQRQTTEPASSTRHTSFKRHLPRQMHVSSVDYGNELPPAAAEQPTSIGRIKPELYKQKSVDGDDAKS EAAKSCGKINFSLRYDYESETLIVRILKAFDLPAKDFCGSSDPYVKIYLLPDRKCKLQTRVHRKTLNPTF DENFHFPVPYEELADRKLHLSVFDFDRFSRHDMIGEVILDNLFEASDLSRETSIWKDIQYATSESVDLGE IMFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSEKSQGHRRIQVQDTHPLWRLQAMKSLVVY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276680
ORF Size	1026 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276680.1, NP_001263609.1
RefSeq Size	1857 bp
RefSeq ORF	1029 bp
Locus ID	54524
MW	39.3 kDa
Gene Summary	May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).[UniProtKB/Swiss-Prot Function]

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