Arhgef2 (NM_001198913) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA408978; GEF; GEF-H1; GEFH1; Lbcl1; Lfc; LFP40; mKIAA0651; P40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231431 representing NM_001198913 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAGCTAAAGATGCCCGCTATACCAACGGCCACCTCTTCACCACCATCTCCGTCTCCGGCATGA CCATGTGCTATGCCTGTAACAAGAGCATCACAGCCAAGGAAGCCCTCATTTGTCCTACATGTAACGTGAC CATCCACAACCGCTGTAAAGACACCCTGGCCAACTGTACCAAGGTCAAGCAGAAGCAACAGAAAGCTGCA CTGCTGAGGAACAACACTGCTTTGCAGTCTGTCTCCCTTCGAACGACCACCAGAGAGCGGCCAACGTCTG CCATTTACCCTTCCGACAGCTTCCGGCAGTCCCTCCTGGGTTCTCGGCGGGGCCTCTCCTCCTTATCTTT GGCCAAAAGTGTTTCCACTACCAACATTGCTGGACATTTCAATGATGAGTCTCCTCTGGGGCTGCGTCAG ATCCTCTCCCAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTGTCCGTGGAATCCCTTATTGATG AAGGTGTAGAAGTGTTCTACAATGAGCTGATGAGCGACTTTGAGATGGATGAGAAGGACTTTGAGGCGGA TTCTTGGAGCCTTGCCGTGGACAGCAGCTTCCTGCAGCAGCACAAAAAGGAAGTGATGAAGAAGCAAGAT GTCATCTATGAGCTGATCCAGACAGAGCTGCACCACGTGAGAACCTTGAAGATTATGACCCGCCTCTTTC GCACTGGGATGCTGGAAGAGTTGCAGATGGAGCCAGAAGTGGTCCAGGGCCTGTTCCCCTGCGTGGATGA ACTCAGTGACATTCACACACGTTTCCTTAATCAGCTTCTGGAACGGCGGCGCCAGGCTCTATGTCCAGGC AGCACCCGGAACTTTGTCATCCATCGTTTGGGTGACTTGCTCATCAGTCAGTTCTCAGGTTCCAATGCTG AGCAGATGCGCAAGACCTACTCAGAGTTCTGCAGCCGCCACACCAAGGCCTTAAAGCTCTATAAGGAGCT GTATGCTCGAGACAAACGCTTCCAACAGTTCATCCGGAAAATGACCCGCTCAGCTGTGTTGAAGCGGCAT GGAGTTCAGGAATGCATTCTCCTGGTGACTCAGCGGATCACCAAATACCCTGTGCTCATCAACAGAATCC TGCAGAATTCCCACGGGGTTGAAGAAGAGTACCAAGACTTGGCGTCAGCCCTAGGACTAGTGAAGGAGTT GTTGTCCAATGTGGACCAGGATGTGCACGAGCTGGAGAAAGAGGCCCGCCTGCAGGAGATTTACAACCGA ATGGATCCCCGGGCTCAGACCCCCGTACCTGGCAAGGGCCCCTTCGGCCGAGATGAACTTTTACGGAGAA AACTTATCCATGAAGGCTGCCTGCTCTGGAAGACAGCCACAGGCCGCTTCAAAGATGTCCTGTTGCTGCT GATGACAGATGTGCTCGTGTTTCTCCAGGAAAAGGACCAGAAATACATTTTCACGTCTCTGGACAAGCCC TCAGTGGTGTCCTTGCAGAACCTCATCGTAAGAGACATAGCCAACCAGGCGAAAGGGATGTTTCTGATTA GTTCTGGACCGCCTGAGATGTATGAGGTGCATGCAGCGTCCCGAGACGACCGGACTACCTGGATCCGTGT CATCCAGCAGAGTGTGCGCCTGTGCCCGTCCAGGGAGGACTTTCCTCTGATCGAGACAGAGGATAAGGCG TATCTCCGGAGGATCAAGACGAAACTGCAGCAGAAAAACCAGGCGCTAGTGGAGCTGCTACAGAAGAATG TTGAGCTGTTTGCCGAGATGGTCCACTTCCAGGCCTTAAAGGCTGGCTTCGTTGGAATGCCCCCACCCGC CCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTTGAGTCCCTCCGAGGCGAGCGCCTGCTAAAGGATGCC CTCCGTGAAGTGGAAGGCCTGAAAGACCTGCTGTTGGGCCCATGTGTGGACCTGCCTATGACATCCCGAG AACCAGCCTTACCCTTGGACTCTGACAGCGGTAGCTGTCCTGGGGTCACTGCCAATGGAGAGGCCAGAAC CTTCAATGGCTCCATTGAACTCTGTAGAGCAGACTCGGATTCCAGCCAGAAGGATCGGAATGGAAATCAG TTGAGATCACCACAGGAGGAGGTGTTACAGCCATTGATCAATCTTTATGGACTTCTACATGGCCTGCAGG CTGTTGTGGTCCAGCAGGAAAGACTGATGGAAGCCCTGTTCCCTGAGGGCCCTGAACGGTGGGAAAAGCT ATCCCGAGCCAACTCTCGGGATGGTGAAGCTGGCCGGGCTGCGGTTGCTTCTGTAACTCCTGAGAAGCAG GCCACGGAGCTGGCACTACTGCAGAGGCAACACACCCTGTTGCAGGAAGAGCTGCGGCGCTGCCAGCGGC TCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCTGGAGGCCAGGCTCCGAGAGAGCGAGCAAGCCCGGGC CCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGGCAGCTTGCAGCCTTGGGCCAAAACGAGCCACTCCCG GCAGAAGCGCCCTGGGCTCGCAGGCCTCTGGACCCACGGCGCCGCAGCCTTCCAGCGGGCGACGCTTTAT ACTTGAGCTTCAATCCCCCCCAGCCCAGTCGAGGCCATGACCGCCTGGATTTGCCTGTGACTGTTCGTTC CCTCCACCGACCCTTTGATGACCGAGAGGCGCAAGAACTTGGTAGCCCCGAGGATCGACTACAGGACAGC AGTGACCCTGATACTGGTAGTGAGGAGGAAGTCAGTAGCCGCCTGTCTCCACCTCACAGTCCTCGAGACT TCACCCGAATGCAGGACATTCCTGAAGAGACAGAAAGCCGAGATGGGGAGCCCACAGCTTCAGAGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231431 representing NM_001198913 Red=Cloning site Green=Tags(s) MKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAA LLRNNTALQSVSLRTTTRERPTSAIYPSDSFRQSLLGSRRGLSSLSLAKSVSTTNIAGHFNDESPLGLRQ ILSQSTDSLNMRNRTLSVESLIDEGVEVFYNELMSDFEMDEKDFEADSWSLAVDSSFLQQHKKEVMKKQD VIYELIQTELHHVRTLKIMTRLFRTGMLEELQMEPEVVQGLFPCVDELSDIHTRFLNQLLERRRQALCPG STRNFVIHRLGDLLISQFSGSNAEQMRKTYSEFCSRHTKALKLYKELYARDKRFQQFIRKMTRSAVLKRH GVQECILLVTQRITKYPVLINRILQNSHGVEEEYQDLASALGLVKELLSNVDQDVHELEKEARLQEIYNR MDPRAQTPVPGKGPFGRDELLRRKLIHEGCLLWKTATGRFKDVLLLLMTDVLVFLQEKDQKYIFTSLDKP SVVSLQNLIVRDIANQAKGMFLISSGPPEMYEVHAASRDDRTTWIRVIQQSVRLCPSREDFPLIETEDKA YLRRIKTKLQQKNQALVELLQKNVELFAEMVHFQALKAGFVGMPPPALPRGLFRLESFESLRGERLLKDA LREVEGLKDLLLGPCVDLPMTSREPALPLDSDSGSCPGVTANGEARTFNGSIELCRADSDSSQKDRNGNQ LRSPQEEVLQPLINLYGLLHGLQAVVVQQERLMEALFPEGPERWEKLSRANSRDGEAGRAAVASVTPEKQ ATELALLQRQHTLLQEELRRCQRLGEERATEAGSLEARLRESEQARALLEREAEEIRRQLAALGQNEPLP AEAPWARRPLDPRRRSLPAGDALYLSFNPPQPSRGHDRLDLPVTVRSLHRPFDDREAQELGSPEDRLQDS SDPDTGSEEEVSSRLSPPHSPRDFTRMQDIPEETESRDGEPTASES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198913
ORF Size	2868 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198913.2
RefSeq Size	4284 bp
RefSeq ORF	2871 bp
Locus ID	16800
UniProt ID	Q60875
MW	109 kDa
Gene Summary	Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (PubMed:28453519).[UniProtKB/Swiss-Prot Function]

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