Arhgef2 (NM_001198913) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA408978; GEF; GEF-H1; GEFH1; Lbcl1; Lfc; LFP40; mKIAA0651; P40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229220 representing NM_001198913 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAAGCTAAAGATGCCCGCTATACCAACGGCCACCTCTTCACCACCATCTCCGTCTCCGGCATGA CCATGTGCTATGCCTGTAACAAGAGCATCACAGCCAAGGAAGCCCTCATTTGTCCTACATGTAACGTGAC CATCCACAACCGCTGTAAAGACACCCTGGCCAACTGTACCAAGGTCAAGCAGAAGCAACAGAAAGCTGCA CTGCTGAGGAACAACACTGCTTTGCAGTCTGTCTCCCTTCGAACGACCACCAGAGAGCGGCCAACGTCTG CCATTTACCCTTCCGACAGCTTCCGGCAGTCCCTCCTGGGTTCTCGGCGGGGCCTCTCCTCCTTATCTTT GGCCAAAAGTGTTTCCACTACCAACATTGCTGGACATTTCAATGATGAGTCTCCTCTGGGGCTGCGTCAG ATCCTCTCCCAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTGTCCGTGGAATCCCTTATTGATG AAGGTGTAGAAGTGTTCTACAATGAGCTGATGAGCGACTTTGAGATGGATGAGAAGGACTTTGAGGCGGA TTCTTGGAGCCTTGCCGTGGACAGCAGCTTCCTGCAGCAGCACAAAAAGGAAGTGATGAAGAAGCAAGAT GTCATCTATGAGCTGATCCAGACAGAGCTGCACCACGTGAGAACCTTGAAGATTATGACCCGCCTCTTTC GCACTGGGATGCTGGAAGAGTTGCAGATGGAGCCAGAAGTGGTCCAGGGCCTGTTCCCCTGCGTGGATGA ACTCAGTGACATTCACACACGTTTCCTTAATCAGCTTCTGGAACGGCGGCGCCAGGCTCTATGTCCAGGC AGCACCCGGAACTTTGTCATCCATCGTTTGGGTGACTTGCTCATCAGTCAGTTCTCAGGTTCCAATGCTG AGCAGATGCGCAAGACCTACTCAGAGTTCTGCAGCCGCCACACCAAGGCCTTAAAGCTCTATAAGGAGCT GTATGCTCGAGACAAACGCTTCCAACAGTTCATCCGGAAAATGACCCGCTCAGCTGTGTTGAAGCGGCAT GGAGTTCAGGAATGCATTCTCCTGGTGACTCAGCGGATCACCAAATACCCTGTGCTCATCAACAGAATCC TGCAGAATTCCCACGGGGTTGAAGAAGAGTACCAAGACTTGGCGTCAGCCCTAGGACTAGTGAAGGAGTT GTTGTCCAATGTGGACCAGGATGTGCACGAGCTGGAGAAAGAGGCCCGCCTGCAGGAGATTTACAACCGA ATGGATCCCCGGGCTCAGACCCCCGTACCTGGCAAGGGCCCCTTCGGCCGAGATGAACTTTTACGGAGAA AACTTATCCATGAAGGCTGCCTGCTCTGGAAGACAGCCACAGGCCGCTTCAAAGATGTCCTGTTGCTGCT GATGACAGATGTGCTCGTGTTTCTCCAGGAAAAGGACCAGAAATACATTTTCACGTCTCTGGACAAGCCC TCAGTGGTGTCCTTGCAGAACCTCATCGTAAGAGACATAGCCAACCAGGCGAAAGGGATGTTTCTGATTA GTTCTGGACCGCCTGAGATGTATGAGGTGCATGCAGCGTCCCGAGACGACCGGACTACCTGGATCCGTGT CATCCAGCAGAGTGTGCGCCTGTGCCCGTCCAGGGAGGACTTTCCTCTGATCGAGACAGAGGATAAGGCG TATCTCCGGAGGATCAAGACGAAACTGCAGCAGAAAAACCAGGCGCTAGTGGAGCTGCTACAGAAGAATG TTGAGCTGTTTGCCGAGATGGTCCACTTCCAGGCCTTAAAGGCTGGCTTCGTTGGAATGCCCCCACCCGC CCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTTGAGTCCCTCCGAGGCGAGCGCCTGCTAAAGGATGCC CTCCGTGAAGTGGAAGGCCTGAAAGACCTGCTGTTGGGCCCATGTGTGGACCTGCCTATGACATCCCGAG AACCAGCCTTACCCTTGGACTCTGACAGCGGTAGCTGTCCTGGGGTCACTGCCAATGGAGAGGCCAGAAC CTTCAATGGCTCCATTGAACTCTGTAGAGCAGACTCGGATTCCAGCCAGAAGGATCGGAATGGAAATCAG TTGAGATCACCACAGGAGGAGGTGTTACAGCCATTGATCAATCTTTATGGACTTCTACATGGCCTGCAGG CTGTTGTGGTCCAGCAGGAAAGACTGATGGAAGCCCTGTTCCCTGAGGGCCCTGAACGGTGGGAAAAGCT ATCCCGAGCCAACTCTCGGGATGGTGAAGCTGGCCGGGCTGCGGTTGCTTCTGTAACTCCTGAGAAGCAG GCCACGGAGCTGGCACTACTGCAGAGGCAACACACCCTGTTGCAGGAAGAGCTGCGGCGCTGCCAGCGGC TCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCTGGAGGCCAGGCTCCGAGAGAGCGAGCAAGCCCGGGC CCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGGCAGCTTGCAGCCTTGGGCCAAAACGAGCCACTCCCG GCAGAAGCGCCCTGGGCTCGCAGGCCTCTGGACCCACGGCGCCGCAGCCTTCCAGCGGGCGACGCTTTAT ACTTGAGCTTCAATCCCCCCCAGCCCAGTCGAGGCCATGACCGCCTGGATTTGCCTGTGACTGTTCGTTC CCTCCACCGACCCTTTGATGACCGAGAGGCGCAAGAACTTGGTAGCCCCGAGGATCGACTACAGGACAGC AGTGACCCTGATACTGGTAGTGAGGAGGAAGTCAGTAGCCGCCTGTCTCCACCTCACAGTCCTCGAGACT TCACCCGAATGCAGGACATTCCTGAAGAGACAGAAAGCCGAGATGGGGAGCCCACAGCTTCAGAGAGCTA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198913
Insert Size	2871 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198913.1, NP_001185842.1
RefSeq Size	4284 bp
RefSeq ORF	2871 bp
Locus ID	16800
UniProt ID	Q60875
Gene Summary	Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (PubMed:28453519).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) has multiple differences at its 5' end and uses a downstream in-frame translation initiation site, compared to variant 1. Variant 4 encodes a shorter protein (isoform 4), compared to isoform 1.

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