Ogdh (NM_001252288) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2210403E04Rik; 2210412K19Rik; AA409584; d1401; mKIAA4192
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231492 representing NM_001252288 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCATTTAAGGACTTGTGCTGCTAAGTTAAGGCCATTGACAGCCTCCCAGACTGTTAAGACATTTT CACAAAACAAACCAGCAGCAATTAGGACGTTTCAACAGATTCGGTGCTATTCTGCACCTGTAGCTGCTGA ACCATTTCTTAGTGGGACTAGTTCGAACTATGTGGAGGAAATGTACTGTGCCTGGTTGGAGAATCCCAAA AGTGTACATAAGTCATGGGACATTTTTTTCCGAAACACCAATGCTGGAGCCCCACCGGGCACTGCCTACC AGAGCCCCCTTTCCCTGAGTCGAAGCTCCCTGGCTACCATGGCCCATGCACAGTCCCTGGTGGAAGCACA ACCTAACGTCGACAAACTCGTGGAGGACCACTTGGCGGTGCAGTCTCTCATCAGGGCATATCAGGTCAGG GGTCACCACATTGCAAAACTTGATCCTCTCGGAATTAGTTGTGTAAATTTTGATGATGCTCCGGTAACTG TTTCTTCAAACGTGGGGTTCTATGGCCTACACGAGTCTGACCTTGACAAGGTCTTCCACTTACCCACCAC CACTTTCATCGGGGGACAGGAGCCAGCACTTCCTCTTCGGGAGATCATCCGTCGGCTGGAGATGGCCTAC TGCCAGCACATTGGTGTGGAGTTCATGTTCATTAATGATTTGGAACAATGCCAGTGGATCCGACAGAAGT TTGAGACCCCTGGAATCATGCAGTTCACCAATGAGGAGAAGCGGACCTTGCTGGCCAGGCTTGTACGATC CACCAGGTTTGAGGAGTTCCTACAGCGAAAGTGGTCCTCGGAGAAGCGTTTTGGTCTGGAAGGCTGTGAG GTGCTGATCCCTGCCCTCAAGACAATCATTGATATGTCAAGTGCAAATGGAGTGGACTATGTGATCATGG GGATGCCACACAGAGGACGGCTGAACGTGCTTGCAAATGTCATCAGGAAGGAGCTGGAGCAAATATTCTG TCAGTTTGACTCAAAGCTGGAGGCAGCTGATGAGGGTTCTGGGGACATGAAGTACCACCTGGGCATGTAT CACCGCAGGATCAACCGTGTGACCGACAGAAACATCACTTTGTCCTTGGTGGCTAACCCTTCCCATCTAG AGGCTGCTGACCCTGTCGTGATGGGAAAGACCAAAGCTGAACAGTTCTACTGTGGAGACACTGAAGGGAA AAAGGTGATGTCTATCCTGCTGCATGGGGATGCTGCTTTTGCTGGCCAGGGCATCGTATATGAGACCTTC CATCTCAGCGACTTGCCGTCCTACACAACCCATGGCACTGTTCATGTGGTTGTCAACAACCAGATTGGCT TCACCACAGACCCTCGGATGGCCCGCTCCTCTCCCTACCCCACTGATGTGGCCCGAGTGGTGAATGCCCC CATTTTCCATGTCAACTCAGATGACCCTGAAGCTGTCATGTATGTATGCAAGGTGGCAGCTGAGTGGAGA AACACCTTCCACAAGGATGTTGTAGTTGATCTGGTGTGTTATCGACGAAATGGCCACAATGAGATGGACG AACCTATGTTTACACAGCCACTCATGTACAAGCAGATCCGCAAGCAGAAGCCTGTACTGCAGAAGTATGC AGAATTGCTAGTCTCCCAGGGTGTCGTCAATCAGCCTGAGTACGAGGAGGAAATCTCCAAGTATGATAAG ATCTGTGAGGAAGCATTTACCAGATCCAAAGATGAGAAGATCTTGCACATCAAGCACTGGCTGGATTCCC CCTGGCCTGGCTTTTTCACCCTGGATGGACAGCCCAGGAGCATGACCTGCCCCTCCACTGGCCTGGAGGA GGATGTCTTGTTCCACATTGGAAAGGTGGCCAGCTCTGTACCTGTGGAGAACTTTACTATCCATGGAGGG CTGAGCCGGATCTTGAAGACCCGCAGAGAGCTTGTGACGAACCGGACTGTGGACTGGGCCCTGGCAGAGT ACATGGCATTTGGCTCACTGCTGAAGGAAGGCATCCATGTGCGGCTGAGTGGCCAGGATGTGGAGCGGGG CACCTTCAGCCATCGCCACCATGTGCTCCATGATCAGAATGTTGACAAAAGAACCTGCATCCCCATGAAC CACCTTTGGCCAAATCAGGCCCCTTACACTGTATGCAACAGCTCGCTGTCTGAGTACGGTGTCCTGGGCT TTGAGCTGGGCTTTGCCATGGCTAGCCCTAATGCTCTGGTTCTCTGGGAGGCCCAGTTTGGTGACTTCAA CAACATGGCACAGTGCATCATTGACCAGTTCATCTGCCCAGGACAGGCAAAGTGGGTGCGGCAGAATGGC ATTGTGCTCCTGCTGCCTCATGGCATGGAAGGCATGGGTCCCGAGCATTCCTCTGCCCGCCCAGAGCGGT TTCTGCAGATGTGCAATGATGACCCAGATGTCCTGCCTGACCTGCAGGAAGAAAACTTTGACATCAATCA GCTCTATGACTGCAACTGGATTGTTGTCAACTGTTCCACCCCTGGCAACTTCTTCCATGTGCTGCGACGA CAGATCTTGCTGCCCTTCCGGAAGCCGTTAATCGTCTTCACTCCCAAATCCCTCCTGCGCCACCCTGAGG CAAGAACTAGCTTTGACGAGATGCTGCCAGGAACGCACTTCCAGCGTGTGATCCCAGAAAATGGACCTGC AGCTCAGGACCCACACAAAGTCAAGAGACTTCTCTTCTGCACTGGGAAGGTGTACTATGACCTCACCCGA GAGCGCAAAGCCAGGAACATGGAGGAGGAGGTGGCTATTACAAGGATTGAGCAGCTATCACCATTCCCCT TTGACCTCCTGCTGAAAGAGGCACAGAAGTATCCCAATGCTGAGCTGGCCTGGTGCCAGGAAGAGCACAA GAACCAAGGCTACTATGACTATGTCAAGCCAAGACTTCGTACCACCATTGACCGTGCTAAGCCTGTCTGG TATGCTGGCCGAGACCCGGCAGCTGCTCCAGCCACTGGCAACAAGAAAACACACCTGACAGAGCTGCAGC GCTTTCTGGACACAGCCTTTGACCTGGACGCATTCAAGAAATTCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231492 representing NM_001252288 Red=Cloning site Green=Tags(s) MFHLRTCAAKLRPLTASQTVKTFSQNKPAAIRTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPK SVHKSWDIFFRNTNAGAPPGTAYQSPLSLSRSSLATMAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQVR GHHIAKLDPLGISCVNFDDAPVTVSSNVGFYGLHESDLDKVFHLPTTTFIGGQEPALPLREIIRRLEMAY CQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCE VLIPALKTIIDMSSANGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDMKYHLGMY HRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETF HLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWR NTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDK ICEEAFTRSKDEKILHIKHWLDSPWPGFFTLDGQPRSMTCPSTGLEEDVLFHIGKVASSVPVENFTIHGG LSRILKTRRELVTNRTVDWALAEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMN HLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFNNMAQCIIDQFICPGQAKWVRQNG IVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLQEENFDINQLYDCNWIVVNCSTPGNFFHVLRR QILLPFRKPLIVFTPKSLLRHPEARTSFDEMLPGTHFQRVIPENGPAAQDPHKVKRLLFCTGKVYYDLTR ERKARNMEEEVAITRIEQLSPFPFDLLLKEAQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTIDRAKPVW YAGRDPAAAPATGNKKTHLTELQRFLDTAFDLDAFKKFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252288
ORF Size	3057 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252288.1, NP_001239217.1
RefSeq Size	6678 bp
RefSeq ORF	3060 bp
Locus ID	18293
UniProt ID	Q60597
MW	116.5 kDa
Gene Summary	2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.[UniProtKB/Swiss-Prot Function]

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