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SAP62 (SF3A2) (NM_007165) Human Tagged ORF Clone
CAT＃: RC201244
- TrueORF®
SF3A2 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 2, 66kDa (SF3A2)
ORF Plasmid: tGFP
"NM_007165" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PRP11; PRPF11; SAP62; SF3a66 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201244 representing NM_007165
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTCCAGCATCGCCCCGGGGGCAAGACCGGGAGCGGGGGCGTGGCCTCCTCCTCCGAGAGCAACC GTGACCGCAGGGAGCGCCTCCGGCAGCTGGCCCTGGAGACCATCGACATCAACAAGGACCCGTACTTCAT GAAGAACCACCTGGGCTCCTATGAATGCAAACTCTGCCTGACACTTCACAACAATGAGGGGAGCTACCTG GCACATACGCAGGGGAAGAAGCACCAGACCAACCTGGCCCGGCGAGCAGCCAAGGAGGCCAAGGAGGCCC CTGCCCAGCCCGCGCCTGAGAAGGTCAAGGTGGAGGTGAAGAAGTTTGTGAAGATCGGCCGCCCGGGCTA CAAAGTGACCAAGCAGAGAGACTCGGAGATGGGCCAGCAGAGCCTCCTCTTCCAGATTGACTACCCTGAG ATCGCCGAGGGCATCATGCCACGTCACCGCTTCATGTCTGCGTACGAGCAGAGGATCGAGCCTCCGGACC GGCGCTGGCAGTACCTGCTCATGGCCGCCGAGCCCTACGAGACCATTGCCTTCAAGGTGCCGAGCAGAGA GATCGACAAGGCGGAGGGCAAGTTCTGGACACACTGGAACCGGGAGACCAAGCAGTTCTTCCTCCAGTTC CACTTTAAGATGGAGAAGCCCCCGGCTCCACCCAGCCTCCCTGCTGGCCCCCCTGGGGTGAAGCGGCCTC CACCCCCGCTGATGAACGGTCTGCCCCCTCGGCCACCGCTGCCTGAGTCTTTGCCACCGCCCCCGCCAGG AGGCCTGCCTCTGCCACCCATGCCCCCCACAGGGCCTGCGCCCTCAGGGCCCCCGGGACCACCCCAGCTA CCCCCGCCAGCTCCAGGGGTCCACCCCCCGGCCCCAGTGGTGCATCCCCCTGCATCTGGGGTCCATCCCC CAGCTCCTGGCGTCCACCCCCCAGCTCCTGGCGTCCATCCCCCAGCCCCTGGGGTCCACCCACCAACCTC TGGGGTCCACCCCCCAGCTCCTGGAGTCCACCCTCCAGCCCCCGGGGTTCACCCACCAGCCCCCGGAGTC CACCCACCAGCCCCTGGGGTTCACCCACCAGCCCCAGGGGTCCATCCTCCCCCATCAGCGGGGGTTCACC CCCAGGCCCCGGGGGTGCACCCAGCAGCCCCCGCCGTTCACCCTCAGGCCCCAGGGGTGCACCCACCAGC CCCAGGGATGCACCCTCAGGCCCCGGGGGTCCACCCCCAACCTCCCGGGGTCCATCCGTCGGCTCCTGGG GTCCACCCTCAGCCTCCGGGAGTTCACCCCTCAAATCCTGGGGTGCACCCCCCAACTCCCATGCCCCCAA TGCTGAGGCCCCCACTTCCCTCCGAAGGCCCAGGGAACATACCTCCCCCTCCCCCAACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201244 representing NM_007165
Red=Cloning site Green=Tags(s) MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEGSYL AHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDSEMGQQSLLFQIDYPE IAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQF HFKMEKPPAPPSLPAGPPGVKRPPPPLMNGLPPRPPLPESLPPPPPGGLPLPPMPPTGPAPSGPPGPPQL PPPAPGVHPPAPVVHPPASGVHPPAPGVHPPAPGVHPPAPGVHPPTSGVHPPAPGVHPPAPGVHPPAPGV HPPAPGVHPPAPGVHPPPSAGVHPQAPGVHPAAPAVHPQAPGVHPPAPGMHPQAPGVHPQPPGVHPSAPG VHPQPPGVHPSNPGVHPPTPMPPMLRPPLPSEGPGNIPPPPPTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007165 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007165.5 |
| RefSeq Size | 1678 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 8175 |
| UniProt ID | Q15428 |
| Domains | ZnF_U1 |
| Protein Pathways | Spliceosome |
| MW | 49.1 kDa |
| Gene Summary | This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201244L3 | Lenti-ORF clone of SF3A2 (Myc-DDK-tagged)-Human splicing factor 3a, subunit 2, 66kDa (SF3A2) |
CNY 5,890.00 |
|
| RC201244L4 | Lenti-ORF clone of SF3A2 (mGFP-tagged)-Human splicing factor 3a, subunit 2, 66kDa (SF3A2) |
CNY 5,890.00 |
|
| RG201244 | SF3A2 (tGFP-tagged) - Human splicing factor 3a, subunit 2, 66kDa (SF3A2) |
CNY 4,370.00 |
|
| SC115656 | SF3A2 (untagged)-Human splicing factor 3a, subunit 2, 66kDa (SF3A2) |
CNY 2,950.00 |
