MAPK6 (NM_002748) Human Tagged Lenti ORF Clone

CAT#: RC201597L4

  • LentiORF®

Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged



  "NM_002748" in other vectors (7)

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CNY 7,700.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Anti-ERK3 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)
    • 100 ul

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Synonyms ERK3; HsT17250; p97MAPK; PRKM6
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC201597).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002748
ORF Size 2163 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002748.2
RefSeq Size 4225 bp
RefSeq ORF 2166 bp
Locus ID 5597
UniProt ID Q16659
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
MW 82.7 kDa
Gene Summary The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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