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MAPK6 (NM_002748) Human Untagged Clone
CAT＃: SC323360
MAPK6 (untagged)-Kinase deficient mutant (K49M) of Human mitogen-activated protein kinase 6 (MAPK6)
CNY 5,288.00
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ERK3; HsT17250; p97MAPK; PRKM6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323360 sequence for NM_002748 edited (data generated by NextGen Sequencing)
ATGGCAGAGAAATTTGAAAGTCTCATGAACATTCATGGTTTTGATCTGGGTTCTAGGTAT ATGGACTTAAAACCATTGGGTTGTGGAGGCAATGGCTTGGTTTTTTCTGCTGTAGACAAT GACTGTGACAAAAGAGTAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAA CATGCTCTACGTGAAATCAAAATTATTAGAAGACTTGACCATGATAACATTGTGAAAGTG TTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACAGACGATGTGGGCTCTCTTACGGAA CTGAACAGTGTTTACATTGTTCAGGAGTACATGGAGACAGACTTGGCTAATGTGCTGGAG CAGGGCCCTTTACTGGAAGAGCATGCCAGGCTTTTCATGTATCAGCTGCTACGGGGGCTC AAGTATATTCACTCTGCAAATGTACTGCACAGAGATCTCAAACCAGCTAATCTTTTCATT AATACGGAAGACTTGGTGCTGAAGATAGGTGACTTTGGTCTTGCACGGATCATGGATCCT CATTATTCCCATAAGGGTCATCTTTCTGAAGGATTGGTTACTAAATGGTACAGATCTCCA CGTCTTTTACTTTCTCCTAATAATTATACTAAAGCCATTGACATGTGGGCTGCAGGCTGC ATCTTTGCTGAAATGCTGACTGGTAAAACCCTTTTTGCAGGTGCACATGAACTTGAACAG ATGCAGCTGATTTTAGAATCTATTCCTGTTGTACATGAGGAAGATCGTCAGGAGCTTCTC AGCGTAATTCCAGTTTACATTAGAAATGACATGACTGAGCCACACAAACCTTTAACTCAG CTGCTTCCAGGAATTAGTCGAGAAGCAGTGGATTTCCTGGAACAAATTTTGACATTTAGC CCCATGGATCGGTTAACAGCAGAAGAAGCACTCTCCCATCCTTACATGAGCATATATTCT TTTCCAATGGATGAGCCAATTTCAAGCCATCCTTTTCATATTGAAGATGAAGTTGATGAT ATTTTGCTTATGGATGAAACTCACAGTCACATTTATAACTGGGAAAGGTATCATGATTGT CAGTTTTCAGAGCATGATTGGCCTGTACATAACAACTTTGATATTGATGAAGTTCAGCTT GATCCAAGAGCTCTGTCCGATGTCACTGATGAAGAAGAAGTACAAGTTGATCCCCGAAAA TATTTGGATGGAGATCGGGAAAAGTATCTGGAGGATCCTGCTTTTGATACCAATTACTCT ACTGAGCCTTGTTGGCAATACTCAGATCATCATGAAAACAAATATTGTGATCTGGAGTGT AGCCATACTTGTAACTACAAAACGAGGTCATCATCATATTTAGATAACTTAGTTTGGAGA GAGAGTGAAGTTAACCATTACTATGAACCCAAGCTTATTATAGATCTTTCCAATTGGAAA GAACAAAGCAAAGAAAAATCTGATAAGAAAGGCAAATCAAAATGTGAAAGGAATGGATTG GTTAAAGCCCAGATAGCGCTAGAGGAAGCATCACAGCAACTGGCTGGAAAAGAAAGGGAA AAGAATCAGGGATTTGATTTTGATTCCTTTATTGCAGGAACTATTCAGCTTAGTTCCCAG CATGAGCCTACTGATGTTGTTGATAAATTAAATGACTTGAATAGCTCAGTGTCCCAACTA GAATTGAAAAGTTTGATATCAAAGTCAGTAAGCCAAGAAAAACAGGAAAAAGGAATGGCA AATCTGGCTCAATTAGAAGCCTTGTACCAGTCTTCTTGGGACAGCCAGTTTGTGAGTGGT GGGGAGGACTGTTTTTTCATAAATCAGTTTTGTGAGGTAAGGAAGGATGAACAAGTTGAG AAGGAAAACACTTACACTAGTTACTTGGACAAGTTCTTTAGCAGGAAAGAAGATACTGAA ATGCTAGAAACTGAGCCAGTAGAGGATGGGAAGCTTGGGGAGAGAGGACATGAGGAAGGA TTTCTGAACAACAGTGGGGAGTTCCTCTTTAACAAGCAGCTCGAGTCCATAGGCATCCCA CAGTTTCACAGTCCAGTTGGGTCACCACTTAAGTCAATACAGGCCACATTAACACCTTCT GCTATGAAATCTTCCCCTCAAATTCCTCATCAAACATACAGCAGCATTCTGAAACATCTG AACTAA Clone variation with respect to NM_002748.3 146 a=>t;868 c=>g >OriGene 5' read for mutant NM_002748 unedited
AAGGAAGAGGGCGAGAAGACGACGTGGGGGAGGGAGGGCAACAGCAGGCAGGCAGCGAAAGTAGACAACG ACAGAAACAAAGAGGGAGGAGATGGACGAGGCGACGGGAAGCGAGAGCCCGGGAAGCAGAGCACCGCAAG CAAAGAACAGAGTAAAACAGAAGAGCCAGAAGCTTAGAAACACACGAAGAGGACGGGGAAGACCGAAGGA CAAAGGAAGAAGGGAGGGAGCCCCGACAGGGAAAGGGGGGGGGCGGGGGCCACCAGGACACCAAACAAAG AAAACCCACGGCAAACCAAACAGCCGGCAGGGGCCCCCGGCGAAAAAAAAAAAAAACCACCAAGGGAAAA AAAGAAACCAAAACGGAAAAAGCGGCCAACAGCCCCTCCAGAAAGGAGGCCGCCTCAGGACCCGTTCCAC ACTGGATGGTTTTTGCACTGTTGAGGAAAAAGGATATTAGAAGCCTGGTTGGTGTGCATATTTTATTTCA CATTTTTTGTTAAATGTTAAATCGTTTTAGCACGGTAATCTGAGTTGCACAGTATGTCATTTCATTTCCG TTTTGAGTTTTCTTTGTTTTCGTTAAATGTCTGCAGAGTTGCTGCCCCTTTCTTGAACTATGAGTACTGC AATCTTTTTAATTCTCAATATGAATAGAGCTTTTTGAGCTTTAAATCTAAGGGGAACTCGACAGGCCTGT TTGGCATATGCAATGAACATCAAGAAACCATCTTGCTGTGGAAGCATAATTATTTTTCTTCTCCTTTTGA AGATCTTTCCTTTGATGCCAGTTTTCTTCTTGTTACACCAAGTTCATTTGAAAGAAAAGCATAGTAAGTT TCAAATGCAGAGAATTTGAAGTCTCATGACATCATGTTTGATCTGGTTCTAGTATATGACTAAACATTGG TTGTGGAGGCATTGCCT >SC323360 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
AGAATTAACATTGGGTTGTGGAGGCATGGCTTGGTTTTTTCTGCTGTAGACAATGACTGTGACAAAAGAG TAGCCATCATGAAAATTGTCCTTACTGATCCCCAGAGTGTCAAACATGCTCTACGTGAAATCAAAATTAT TAGAAGACTTGACCATGATAACATTGTGAAAGTGTTTGAGATTCTTGGTCCCAGTGGAAGCCAATTAACA GACGATGTGGGCTCTCTTACGGAACTGAACAGTGTTTACATTGTT |
| Restriction Sites | Please inquire |
| ACCN | NM_002748 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002748.2, NP_002739.1 |
| RefSeq Size | 4193 bp |
| RefSeq ORF | 2166 bp |
| Locus ID | 5597 |
| UniProt ID | Q16659 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201597 | MAPK6 (Myc-DDK-tagged)-Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 5,280.00 |
|
| RC201597L1 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged |
CNY 7,680.00 |
|
| RC201597L2 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged |
CNY 7,700.00 |
|
| RC201597L3 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC201597L4 | Lenti ORF clone of Human mitogen-activated protein kinase 6 (MAPK6), mGFP tagged |
CNY 7,700.00 |
|
| RG201597 | MAPK6 (tGFP-tagged) - Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 6,370.00 |
|
| SC118465 | MAPK6 (untagged)-Human mitogen-activated protein kinase 6 (MAPK6) |
CNY 5,288.00 |
