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SSX3 (NM_175711) Human Tagged ORF Clone
CAT＃: RC203244
SSX3 (Myc-DDK-tagged)-Human synovial sarcoma, X breakpoint 3 (SSX3), transcript variant 2
ORF Plasmid: tGFP
"NM_175711" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT5.3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203244 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGAGATGACACCTTTGCAAGGAGACCCACGGTTGGTGCTCAAATACCAGAGAAGATACAAAAGG CCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTGGGAAAAGATGAAAGTCTCGGAGAAAATCGT CTATGTGTATATGAAGAGAAAGTATGAGGCCATGACTAAACTAGGTTTCAAGGCCATCCTCCCATCTTTC ATGCGTAATAAACGGGTCACAGACTTCCAGGGGAATGATTTTGATAATGACCCTAACCGTGGGAATCAGG TTCAACGTCCTCAGATGACTTTCGGCAGGCTCCAGGGAATCTTCCCGAAGATCATGCCCAAGAAGCCAGC AGAGGAAGGAAATGTTTCGAAGGAAGTGCCAGAAGCATCTGGCCCACAAAACGATGGGAAACAGCTGTGC CCCCCGGGAAAACCAACTACCTCTGAGAAGATTAACATGATATCTGGTGTCCTTCAGAGATACTGCAGGT TCGGTTCCAGACCACTGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203244 protein sequence
Red=Cloning site Green=Tags(s) MNGDDTFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKVSEKIVYVYMKRKYEAMTKLGFKAILPSF MRNKRVTDFQGNDFDNDPNRGNQVQRPQMTFGRLQGIFPKIMPKKPAEEGNVSKEVPEASGPQNDGKQLC PPGKPTTSEKINMISGVLQRYCRFGSRPLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175711 |
| ORF Size | 510 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175711.1, NP_783642.1 |
| RefSeq Size | 825 bp |
| RefSeq ORF | 512 bp |
| Locus ID | 10214 |
| Protein Families | Transcription Factors |
| MW | 19.5 kDa |
| Gene Summary | The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. [provided by RefSeq, Jul 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203244L3 | Lenti ORF clone of Human synovial sarcoma, X breakpoint 3 (SSX3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203244L4 | Lenti ORF clone of Human synovial sarcoma, X breakpoint 3 (SSX3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG203244 | SSX3 (tGFP-tagged) - Human synovial sarcoma, X breakpoint 3 (SSX3), transcript variant 2 |
CNY 4,370.00 |
|
| SC125740 | SSX3 (untagged)-Human synovial sarcoma, X breakpoint 3 (SSX3), transcript variant 2 |
CNY 2,400.00 |
