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RARS2 (NM_020320) Human Tagged ORF Clone
CAT＃: RC203384
- TrueORF®
RARS2 (Myc-DDK-tagged)-Human arginyl-tRNA synthetase 2, mitochondrial (RARS2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_020320" in other vectors (4)
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CNY 4,304.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ArgRS; DALRD2; PCH6; PRO1992; RARSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203384 representing NM_020320
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGCGGCTTTCGCCGCGCTATTGCTTGCCAGCTTTCCAGAGTGTTGAATCTTCCACCAGAAAACT TGATCACATCAATATCTGCAGTTCCAATTTCCCAAAAAGAAGAAGTAGCTGATTTTCAGCTTTCTGTGGA TTCTTTATTGGAAAAAGACAATGACCATTCAAGACCAGATATTCAAGTTCAAGCCAAGAGACTAGCAGAG AAGCTAAGATGTGATACAGTGGTGAGTGAAATCAGTACTGGTCAAAGGACTGTAAATTTCAAAATAAACA GAGAGCTCTTAACAAAGACAGTGCTACAACAAGTAATTGAAGATGGCTCAAAATATGGATTAAAAAGTGA ACTTTTCTCTGGACTTCCCCAGAAGAAGATTGTGGTTGAATTCAGTTCACCTAATGTTGCCAAAAAATTT CATGTTGGACATTTGCGTTCTACCATCATAGGAAATTTTATAGCAAATCTCAAAGAAGCTTTAGGACATC AAGTAATAAGAATAAATTACCTTGGCGATTGGGGCATGCAGTTTGGTCTTCTGGGAACTGGCTTCCAGCT GTTTGGCTATGAGGAAAAACTGCAGTCCAATCCTCTACAGCATCTCTTTGAAGTTTATGTACAAGTTAAT AAAGAAGCAGCAGATGATAAAAGTGTAGCAAAAGCAGCACAGGAGTTCTTCCAACGATTGGAACTGGGCG ATGTGCAAGCACTTTCACTGTGGCAAAAATTTCGGGACTTGAGCATTGAAGAGTACATTCGGGTTTACAA GCGTCTGGGAGTATATTTTGATGAATATTCAGGAGAATCATTTTATCGTGAAAAATCTCAAGAGGTCTTA AAGTTGCTGGAGAGTAAAGGACTCCTACTGAAAACAATAAAAGGAACGGCTGTAGTAGATCTCTCTGGGA ATGGCGACCCCTCCTCAATTTGTACTGTAATGCGAAGTGATGGGACTTCTCTCTATGCAACCAGAGATCT TGCAGCTGCTATAGATCGAATGGACAAGTATAATTTTGATACAATGATATATGTGACAGATAAAGGACAA AAAAAGCATTTTCAGCAAGTATTCCAAATGCTGAAGATCATGGGATATGACTGGGCAGAAAGGTGCCAGC ACGTGCCCTTTGGAGTAGTACAGGGAATGAAGACTCGAAGAGGAGATGTCACTTTCCTGGAAGATGTTTT AAATGAGATTCAATTAAGGATGCTACAGAACATGGCTTCAATTAAGACAACTAAAGAACTCAAGAACCCA CAAGAGACTGCAGAGAGGGTCGGGCTCGCAGCACTCATTATTCAGGACTTCAAAGGTTTACTCTTATCTG ACTACAAGTTCAGCTGGGATCGTGTTTTCCAGAGTCGCGGGGACACAGGAGTCTTCCTACAGTACACACA CGCCCGCCTCCACAGTTTGGAAGAGACTTTTGGATGTGGGTACCTGAATGACTTCAACACTGCTTGTTTA CAAGAGCCACAGTCTGTTTCAATTCTTCAGCATCTTCTCAGGTTCGACGAGGTGCTTTATAAATCATCTC AGGACTTTCAACCCAGGCATATCGTCAGTTACCTTCTAACTTTAAGTCATCTTGCAGCTGTGGCACACAA AACACTACAAATAAAAGATAGTCCTCCTGAAGTGGCTGGGGCCAGACTTCATCTTTTCAAAGCTGTCCGT TCTGTCCTAGCCAATGGAATGAAACTTCTTGGAATAACACCTGTATGTAGGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203384 representing NM_020320
Red=Cloning site Green=Tags(s) MACGFRRAIACQLSRVLNLPPENLITSISAVPISQKEEVADFQLSVDSLLEKDNDHSRPDIQVQAKRLAE KLRCDTVVSEISTGQRTVNFKINRELLTKTVLQQVIEDGSKYGLKSELFSGLPQKKIVVEFSSPNVAKKF HVGHLRSTIIGNFIANLKEALGHQVIRINYLGDWGMQFGLLGTGFQLFGYEEKLQSNPLQHLFEVYVQVN KEAADDKSVAKAAQEFFQRLELGDVQALSLWQKFRDLSIEEYIRVYKRLGVYFDEYSGESFYREKSQEVL KLLESKGLLLKTIKGTAVVDLSGNGDPSSICTVMRSDGTSLYATRDLAAAIDRMDKYNFDTMIYVTDKGQ KKHFQQVFQMLKIMGYDWAERCQHVPFGVVQGMKTRRGDVTFLEDVLNEIQLRMLQNMASIKTTKELKNP QETAERVGLAALIIQDFKGLLLSDYKFSWDRVFQSRGDTGVFLQYTHARLHSLEETFGCGYLNDFNTACL QEPQSVSILQHLLRFDEVLYKSSQDFQPRHIVSYLLTLSHLAAVAHKTLQIKDSPPEVAGARLHLFKAVR SVLANGMKLLGITPVCRM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020320 |
| ORF Size | 1734 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020320.5 |
| RefSeq Size | 1848 bp |
| RefSeq ORF | 1737 bp |
| Locus ID | 57038 |
| UniProt ID | Q5T160 |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
| MW | 65.5 kDa |
| Gene Summary | This nuclear gene encodes a protein that localizes to the mitochondria, where it catalyzes the transfer of L-arginine to its cognate tRNA, an important step in translation of mitochondrially-encoded proteins. Defects in this gene are a cause of pontocerebellar hypoplasia type 6 (PCH6). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203384L3 | Lenti-ORF clone of RARS2 (Myc-DDK-tagged)-Human arginyl-tRNA synthetase 2, mitochondrial (RARS2), nuclear gene encoding mitochondrial protein |
CNY 6,460.00 |
|
| RC203384L4 | Lenti-ORF clone of RARS2 (mGFP-tagged)-Human arginyl-tRNA synthetase 2, mitochondrial (RARS2), nuclear gene encoding mitochondrial protein |
CNY 6,460.00 |
|
| RG203384 | RARS2 (tGFP-tagged) - Human arginyl-tRNA synthetase 2, mitochondrial (RARS2), nuclear gene encoding mitochondrial protein |
CNY 5,040.00 |
|
| SC122891 | RARS2 (untagged)-Human arginyl-tRNA synthetase 2, mitochondrial (RARS2), nuclear gene encoding mitochondrial protein |
CNY 2,950.00 |
