DKKL1 (NM_014419) Human Tagged ORF Clone
CAT#: RC205209
- TrueORF®
DKKL1 (Myc-DDK-tagged)-Human dickkopf-like 1 (DKKL1), transcript variant 1
ORF Plasmid: tGFP
"NM_014419" in other vectors (4)
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CNY 2,640.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CT34; SGY; SGY-1; SGY1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205209 representing NM_014419
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAAGCCTCCCCACCTGCCCCCGCAAGGCGGCATCTGCTGGTCCTGCTGCTGCTCCTCTCTACCC TGGTGATCCCCTCCGCTGCAGCTCCTATCCATGATGCTGACGCCCAAGAGAGCTCCTTGGGTCTCACAGG CCTCCAGAGCCTACTCCAAGGCTTCAGCCGACTTTTCCTGAAAGGTAACCTGCTTCGGGGCATAGACAGC TTATTCTCTGCCCCCATGGACTTCCGGGGCCTCCCTGGGAACTACCACAAAGAGGAGAACCAGGAGCACC AGCTGGGGAACAACACCCTCTCCAGCCACCTCCAGATCGACAAGATGACCGACAACAAGACAGGAGAGGT GCTGATCTCCGAGAATGTGGTGGCATCCATTCAACCAGCGGAGGGGAGCTTCGAGGGTGATTTGAAGGTA CCCAGGATGGAGGAGAAGGAGGCCCTGGTACCCATCCAGAAGGCCACGGACAGCTTCCACACAGAACTCC ATCCCCGGGTGGCCTTCTGGATCATTAAGCTGCCACGGCGGAGGTCCCACCAGGATGCCCTGGAGGGCGG CCACTGGCTCAGCGAGAAGCGACACCGCCTGCAGGCCATCCGGGATGGACTCCGCAAGGGGACCCACAAG GACGTCCTAGAAGAGGGGACCGAGAGCTCCTCCCACTCCAGGCTGTCCCCCCGAAAGACCCACTTACTGT ACATCCTCAGGCCCTCTCGGCAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205209 representing NM_014419
Red=Cloning site Green=Tags(s) MGEASPPAPARRHLLVLLLLLSTLVIPSAAAPIHDADAQESSLGLTGLQSLLQGFSRLFLKGNLLRGIDS LFSAPMDFRGLPGNYHKEENQEHQLGNNTLSSHLQIDKMTDNKTGEVLISENVVASIQPAEGSFEGDLKV PRMEEKEALVPIQKATDSFHTELHPRVAFWIIKLPRRRSHQDALEGGHWLSEKRHRLQAIRDGLRKGTHK DVLEEGTESSSHSRLSPRKTHLLYILRPSRQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014419 |
ORF Size | 726 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014419.4 |
RefSeq Size | 1034 bp |
RefSeq ORF | 729 bp |
Locus ID | 27120 |
UniProt ID | Q9UK85 |
Protein Families | ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
MW | 27.01 kDa |
Gene Summary | The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has low sequence similarity to the dickkopf-3 protein. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205209L3 | Lenti-ORF clone of DKKL1 (Myc-DDK-tagged)-Human dickkopf-like 1 (DKKL1), transcript variant 1 |
CNY 5,890.00 |
|
RC205209L4 | Lenti-ORF clone of DKKL1 (mGFP-tagged)-Human dickkopf-like 1 (DKKL1), transcript variant 1 |
CNY 5,890.00 |
|
RG205209 | DKKL1 (tGFP-tagged) - Human dickkopf-like 1 (DKKL1), transcript variant 1 |
CNY 4,370.00 |
|
SC304097 | DKKL1 (untagged)-Human dickkopf-like 1 (DKKL1), transcript variant 1 |
CNY 2,400.00 |